Development of biomedical films adhering onto tissues under wet condition

• Project/Area Number: 25289254
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Structural/Functional materials
• Research Institution: National Institute for Materials Science
• Principal Investigator: Taguchi Tetsushi 国立研究開発法人物質・材料研究機構, 国際ナノアーキテクトニクス研究拠点, MANA研究者 (70354264)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
• Keywords: 接着剤 / 接着性多孔膜 / 接着性フィルム / 医療用接着フィルム

Outline of Final Research Achievements

The bonding behavior of hexanoyl (Hx: C6) group-modified alkaline-treated gelatin (HxAlGltn) porous films ((P)HxAlGltn) on the porcine intestine was evaluated. (P)HxAlGltns with various porosities were prepared by the salt-leaching method for various solid-liquid ratios. (P)HxAlGltns bonded more strongly to porcine intestine surfaces than did porous AlGltn films ((P)AlGltns). L929 cells cultured on (P)HxAlGltns showed adhesivity than cells cultured on (P)AlGltns. Faster tissue infiltration and a shorter degradation time of highly porous (P)HxAlGltns were observed after implantation in rat subcutaneous tissues. The angiogenic markers CD34 and α-SMA were highly expressed around (P)HxAlGltns that had high porosity. These results indicated that highly porous (P)HxAlGltns have advantages with respect to not only bonding strength on wet soft tissues, but also angiogenesis.

Research Products

• [Journal Article] Robust sealing of blood vessels with cholesteryl group-modified, Alaska pollock-derived gelatin-based biodegradable sealant under wet conditions (2016)
  • Author(s): Tetsushi T.; Mizuta, R.; Ito, T.; Yoshizawa, K.; Kajiyama
  • Journal Title: J Biomed Nanotechnol Volume: 12 Issue: 1 Pages: 128-134
  • DOI: 10.1166/jbn.2016.2210
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Binding of Lipopolysaccharide and Cholesterol-Modified Gelatin on Supported Lipid Bilayers: Effect of Bilayer Area Confinement and Bilayer Edge Tension (2016)
  • Author(s): Kataoka-Hamai, C.; Kaizuka, Y.; Taguchi, T.
  • Journal Title: Langmuir Volume: 32 Issue: 5 Pages: 1250-1258
  • DOI: 10.1021/acs.langmuir.5b04302
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Enhanced angiogenesis of growth factor-free porous biodegradable adhesive made with hexanoyl group-modified gelatin (2015)
  • Author(s): Yoshizawa, K.; Ryo, M.; Taguchi, T.
  • Journal Title: Biomaterials Volume: 63 Pages: 14-23
  • DOI: 10.1016/j.biomaterials.2015.06.003
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Induction of Intermembrane Adhesion by Incorporation of Synthetic Adhesive Molecules into Cell Membranes (2015)
  • Author(s): Ushiyama, A.; Ono, M.; Kataoka-Hamai, C.; Taguchi, T.; Kaizuka, Y.
  • Journal Title: Langmuir Volume: 31 Issue: 6 Pages: 1988-1998
  • DOI: 10.1021/la504523c
  • Peer Reviewed
• [Journal Article] Enhanced ALP activity of MG63 cells cultured on hydroxyapatite-poly(ethylene glycol) hydrogel composites prepared using EDTA-OH (2015)
  • Author(s): Ito, T.; Sasaki, M.; Taguchi, T.
  • Journal Title: Biomedical Materials Volume: 10 Issue: 1 Pages: 015025-015025
  • DOI: 10.1088/1748-6041/10/1/015025
  • Peer Reviewed
• [Journal Article] Bonding behavior of hydrophobically modified gelatin films on intestinal surface (2014)
  • Author(s): 吉澤恵子、田口哲志
  • Journal Title: J. Bioact. Compat. Polym. Volume: 29 Issue: 6 Pages: 560-571
  • DOI: 10.1177/0883911514553731
  • Peer Reviewed
• [Journal Article] Crosslinking liposomes/cells using cholesteryl group-modified tilapia gelatin (2014)
  • Author(s): Taguchi, T.; Endo, Y.
  • Journal Title: International Journal of Molecular Sciences Volume: 15 Issue: 7 Pages: 13123-34
  • DOI: 10.3390/ijms150713123
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Prevention of catheter infection using a biodegradable tissue adhesive composed of human serum albumin and disuccinimidyl tartrate (2014)
  • Author(s): Taguchi, T.; Okada, M.; Kogai, Y.; Masuda, M.; Shimomura, Y.; Inoue, M.; Ito, T.; Hamahata, T.; Funatogawa, K.; Kirikae, T.; Furuzono, T.
  • Journal Title: Journal of Bioactive and Compatible Polymers Volume: 29 Issue: 3 Pages: 284-297
  • DOI: 10.1177/0883911514528409
  • Peer Reviewed
• [Journal Article] Effects of ultraviolet irradiation on bonding strength between Co-Cr alloy and citric acid-crosslinked gelatin matrix (2014)
  • Author(s): Inoue, M.; Sasaki, M.; Katada, Y.; Taguchi, T.
  • Journal Title: Journal of Biomaterials Applications Volume: 28 Issue: 6 Pages: 880-886
  • DOI: 10.1177/0885328213485140
  • Peer Reviewed
• [Journal Article] Quantitative biocompatibility evaluation of nickel-free high-nitrogen stainless steel in vitro/in vivo (2014)
  • Author(s): Inoue, M.; Sasaki, M.; Katada, Y.; Taguchi, T.
  • Journal Title: Journal of Biomedical Materials Research. Part B, Applied biomaterials Volume: 102B Issue: 1 Pages: 68-72
  • DOI: 10.1002/jbm.b.32982
  • Peer Reviewed
• [Journal Article] An in vivo murine model for screening cranial bone regenerative materials: testing of a novel synthetic collagen gel. (2014)
  • Author(s): Hisako Hikiji, Ken Tomizuka, Tetsushi Taguchi, Hiroyuki Koyama, Daichi Chikazu, Yoshiyuki Mori, Tsuyoshi Takato.
  • Journal Title: J. Mater. Sci.: Mater Med. Volume: 25 Issue: 6 Pages: 1531-1538
  • DOI: 10.1007/s10856-014-5185-5
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 生体軟組織用接着剤の開発動向、課題および今後の展望 (2014)
  • Author(s): 田口哲志
  • Journal Title: 10年後の市場・技術予測とそこから読み解く必然の研究開発テーマ Pages: 312-317
• [Journal Article] 医療用貼付剤・粘着剤・接着剤への要求特性と高機能化 (2014)
  • Author(s): 田口哲志
  • Journal Title: 粘着剤、接着剤の最適設計と適用技術 Volume: 6 Pages: 714-721
• [Journal Article] 医療用接着剤開発の現状と展望 (2014)
  • Author(s): 田口哲志
  • Journal Title: Polyfile Volume: 3 Pages: 30-35
• [Journal Article] Enhanced bonding strength of hydrophobically modified gelatin films on wet blood vessels (2014)
  • Author(s): Keiko Yoshizawa, Tetsushi Taguchi
  • Journal Title: International Journal of Molecular Science Volume: 15 Issue: 2 Pages: 2142-2156
  • DOI: 10.3390/ijms15022142
  • Peer Reviewed
• [Presentation] 生体接着材料の技術動向 (2016)
  • Author(s): 田口哲志
  • Organizer: 情報機構セミナー
  • Place of Presentation: きゅりあん、東京
  • Year and Date: 2016-03-02
  • Invited
• [Presentation] 細胞・組織ナノ環境に浸透する高強度・生体親和性接着剤 (2016)
  • Author(s): 田口哲志
  • Organizer: Nanotech 2016
  • Place of Presentation: 東京ビックサイト
  • Year and Date: 2016-01-27
• [Presentation] 医学応用を目指した生体親和性シーラントの設計と機能 (2016)
  • Author(s): 水野陽介、水田亮、田口哲志
  • Organizer: つくば医工連携フォーラム2016
  • Place of Presentation: 産業技術総合研究所
  • Year and Date: 2016-01-22
• [Presentation] 生体機能界面制御による革新的外科用接着剤の開発 (2016)
  • Author(s): 田口哲志
  • Organizer: つくば医工連携フォーラム2016
  • Place of Presentation: 産業技術総合研究所
  • Year and Date: 2016-01-22
  • Invited
• [Presentation] バイオナノ界面デザインによる生体親和性接着剤の開発 (2016)
  • Author(s): 水田亮、伊藤典明、田口哲志
  • Organizer: 第25回インテリジェント/システムシンポジウム
  • Place of Presentation: 東京女子医科大学 先端生命医科学研究所
  • Year and Date: 2016-01-08
• [Presentation] 生体組織界面制御による革新的外科用接着剤の開発 (2015)
  • Author(s): 田口哲志
  • Organizer: 電気学会 バイオ・マイクロシステム研究会
  • Place of Presentation: 物質・材料研究機構
  • Year and Date: 2015-12-16
  • Invited
• [Presentation] 血管新生能を有する細胞増殖因子フリー組織接着能多孔膜の創製 (2015)
  • Author(s): 田口哲志、水田亮、吉澤恵子、
  • Organizer: 第53回日本人工臓器学会大会
  • Place of Presentation: 東京ドームホテル,東京都
  • Year and Date: 2015-11-19
• [Presentation] 耐圧強度と生体親和性に優れた疎水化タラゼラチンシーラントの設計と機能 (2015)
  • Author(s): 水田亮、伊藤典明、吉澤恵子、田口哲志
  • Organizer: 第37回日本バイオマテリアル学会大会
  • Place of Presentation: 京都テルサ
  • Year and Date: 2015-11-09
• [Presentation] 優れた生体組織界面強度を示す疎水化ゼラチン接着剤の創製 (2015)
  • Author(s): 水田亮、伊藤典明、吉澤恵子、田口哲志
  • Organizer: 第5回CSJ化学フェスタ2015
  • Place of Presentation: タワーホール船堀,東京
  • Year and Date: 2015-10-13
• [Presentation] 湿潤環境で血管組織接着性を示す生体親和性シーラントの創製 (2015)
  • Author(s): 水田亮、伊藤典明、吉澤恵子、田口哲志
  • Organizer: 第64回高分子討論会
  • Place of Presentation: 東北大学,仙台
  • Year and Date: 2015-09-15
• [Presentation] Enhanced interfacial strength of surgical sealants composed of (2015)
  • Author(s): 水田亮、伊藤典明、吉澤恵子、秋山利正、神谷勝弘、田口哲志
  • Organizer: ESB2015 - 27th European Conference on Biomaterials-
  • Place of Presentation: Krakow Congress Centre,Kraków,Poland
  • Year and Date: 2015-08-30
  • Int'l Joint Research
• [Presentation] 湿潤臓器・組織表面へ接着する生体吸収性接着剤の設計と機能 (2015)
  • Author(s): 田口哲志
  • Organizer: 第3回 生体材料接着研究会
  • Place of Presentation: 東京医科歯科大学
  • Year and Date: 2015-01-16
  • Invited
• [Presentation] Hydrophobically Modified Gelatin-based Adhesives for Biomedical Applic (2014)
  • Author(s): 田口哲志
  • Organizer: 2014 Asia University Symposium on Biomedical Engineering
  • Place of Presentation: National Taiwan University
  • Year and Date: 2014-12-13 – 2014-12-14
  • Invited
• [Presentation] Growth factor-free, porous tissue adhesive films with angiogenic activ (2014)
  • Author(s): 吉澤恵子、水田亮、田口哲志
  • Organizer: IPC2014
  • Place of Presentation: エポカルつくば
  • Year and Date: 2014-12-02 – 2014-12-05
• [Presentation] Enhanced interfacial strength of surgical sealants composed of hydroph (2014)
  • Author(s): 水田亮、伊藤典明、吉澤恵子、田口哲志他
  • Organizer: IPC2014
  • Place of Presentation: エポカルつくば
  • Year and Date: 2014-12-02 – 2014-12-05
• [Presentation] 湿潤血管組織に接着する生体親和性シーラントの設計と機能 (2014)
  • Author(s): 水田亮、伊藤典明、吉澤恵子、田口哲志他
  • Organizer: 第36回日本バイオマテリアル学会大会
  • Place of Presentation: タワーホール船堀
  • Year and Date: 2014-11-17 – 2014-11-18
• [Presentation] 血管新生能と組織接着能を併せ持つ疎水化ゼラチン多孔質フィルムの創製 (2014)
  • Author(s): 吉澤恵子、水田亮、田口哲志
  • Organizer: 第36回日本バイオマテリアル学会大会
  • Place of Presentation: タワーホール船堀
  • Year and Date: 2014-11-17 – 2014-11-18
• [Presentation] 湿潤組織・臓器に接着する生体親和性バイオマテリアルの創製 (2014)
  • Author(s): 田口哲志
  • Organizer: 表面技術協会 講演会 -バイオインターフェースとしてのマテリアル
  • Place of Presentation: 東京医科歯科大学
  • Year and Date: 2014-10-24
  • Invited
• [Presentation] 湿潤血管接着性を有する外科用シーラントの開発 (2014)
  • Author(s): 田口哲志、水田亮、伊藤典明、吉澤恵子他
  • Organizer: 第52回日本人工臓器学会大会
  • Place of Presentation: 京王プラザホテル札幌
  • Year and Date: 2014-10-17 – 2014-10-19
• [Presentation] Increased Interfacial Bonding Strength between Soft Tissues and Hydrop (2014)
  • Author(s): 田口哲志
  • Organizer: 5th World Congress on Adhesion and Related Phenomena
  • Place of Presentation: Nara Prefectural New Public Hall
  • Year and Date: 2014-09-07 – 2014-09-11
  • Invited
• [Presentation] Stimulation of angiogenesis by growth factor-free porous adhesive film (2014)
  • Author(s): 吉澤恵子、伊藤典明、水田亮、田口哲志
  • Organizer: ESB 2014
  • Place of Presentation: The Liverpool Echo Arena and Convention Centre
  • Year and Date: 2014-08-31 – 2014-09-03
• [Presentation] Hydrophobically modified, cod-derived gelatins-based surgical sealants (2014)
  • Author(s): 水田亮、伊藤典明、吉澤恵子、田口哲志他
  • Organizer: ESB 2014
  • Place of Presentation: The Liverpool Echo Arena and Convention Centre
  • Year and Date: 2014-08-31 – 2014-09-03
• [Presentation] Effect of hydrophobic groups on the bonding strength of cod-derived ge (2014)
  • Author(s): 田口哲志、伊藤典明、水田亮、吉澤恵子
  • Organizer: ESB 2014
  • Place of Presentation: The Liverpool Echo Arena and Convention Centre
  • Year and Date: 2014-08-31 – 2014-09-03
• [Presentation] Enhanced Bondig of blood vessels using tissue adhesives containing hyd (2014)
  • Author(s): 田口哲志、井上元基、松田美幸
  • Organizer: 6th International Symposium on Functional Materials (ISFM 2014)
  • Place of Presentation: Novotel Singapore Clarke Quay
  • Year and Date: 2014-08-04 – 2014-08-07
• [Presentation] Stimulation of angiogenesis by growth factor-free porous adhesive film (2014)
  • Author(s): 吉澤恵子、伊藤典明、水田亮、田口哲志
  • Organizer: NIMS Conference 2014
  • Place of Presentation: EPOKAL TSUKUBA
  • Year and Date: 2014-07-01 – 2014-07-03
• [Presentation] Hydrophobically modified, cod-derived gelatin-based surgical sealants (2014)
  • Author(s): 水田亮、伊藤典明、吉澤恵子、田口哲志他
  • Organizer: NIMS Conference 2014
  • Place of Presentation: EPOKAL TSUKUBA
  • Year and Date: 2014-07-01 – 2014-07-03
• [Presentation] Enhanced Bonding Strength of Tissue Adhesives composed of Hydrophobica (2014)
  • Author(s): 田口哲志、井上元基、松田美幸
  • Organizer: NIMS Conference 2014
  • Place of Presentation: EPOKAL TSUKUBA
  • Year and Date: 2014-07-01 – 2014-07-03
• [Presentation] 湿潤組織接着性を有する疎水化ゼラチン多孔膜の開発 (2014)
  • Author(s): 吉澤恵子、田口哲志
  • Organizer: 高分子学会年次大会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2014-05-28 – 2014-05-30
• [Presentation] 湿潤血管組織に対する疎水化ゼラチンのシーリング効果 (2014)
  • Author(s): 水田亮、伊藤典明、吉澤恵子、田口哲志他
  • Organizer: 高分子学会年次大会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2014-05-28 – 2014-05-30
• [Presentation] 湿潤組織に接着する架橋剤フリー疎水化ゼラチンフィルムの創製 (2013)
  • Author(s): 吉澤恵子、田口哲志
  • Organizer: 高分子学会年次大会
  • Place of Presentation: 京都国際会館
• [Presentation] 湿潤生体組織に接着するゼラチンフィルムの疎水基導入効果 (2013)
  • Author(s): 吉澤恵子、田口哲志
  • Organizer: 日本接着学会年次大会
  • Place of Presentation: 明治大学駿河台キャンパス
• [Presentation] Bonding befavior of film adhesive composed with hydrophobically-modified gelatin under wet condition (2013)
  • Author(s): Keiko Yoshizawa, Tetsushi Taguchi
  • Organizer: Tsukuba International Conference on Materials Science (TICMS)
  • Place of Presentation: University of Tsukuba
• [Presentation] 湿潤組織接着性を有する架橋剤フリー分解性フィルムの創製 (2013)
  • Author(s): 田口哲志、吉澤恵子
  • Organizer: 第51回日本人工臓器学会大会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Adhesion behavior of hydrophobically-modified gelatin films to soft tissues under wet condition (2013)
  • Author(s): Keiko Yoshizawa, Tetsushi Taguchi
  • Organizer: 25th European Conference on Biomaterials
  • Place of Presentation: Hotel Meliá Castilla,MADRID,Spain
• [Presentation] 疎水基導入ゼラチンフィルムの湿潤組織に対する接着効果 (2013)
  • Author(s): 吉澤恵子、田口哲志
  • Organizer: 第35回日本バイオマテリアル学会大会
  • Place of Presentation: タワーホール船越
• [Patent(Industrial Property Rights)] 組織接着性多孔質膜、その製造方法及び組織接着性多孔質膜テープ (2013)
  • Inventor(s): 田口哲志
  • Industrial Property Rights Holder: 独立行政法人物質・材料研究機構
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-241764
  • Filing Date: 2013-11-22