| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Outline of Final Research Achievements |
An anaerobic bacterium Clostridium cellulovorans produces a large extracellular enzyme complex called cellulosome and a number of non-cellulosomal proteins. Each component plays an important role on efficient degradation of various biomass-substrates. We performed intracellular and extracellular time-course proteome analyses with xylan in which C. cellulovorans changed profile of proteins significantly. C. cellulovorans proteins were successfully identified, and these profiles changed dependent on each culture time. Comparison of the intracellular and extracellular protein profiles indicated that C. cellulovorans adapted each profile of proteins in response to residues of xylan. Furthermore, we clarified the production of some key proteins, which involve in substrate degradation and metabolism.
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