Development of bioprocess for recovering phosphorus from a variety of secondary resources for recycling

• Project/Area Number: 25289294
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Waseda University (2015); Osaka University
• Principal Investigator: Ohtake Hisao 早稲田大学, 総合研究機構, 客員上級研究員 (10127483)
• Co-Investigator(Kenkyū-buntansha): OKANO KENJI 大阪大学, 工学研究科, 助教 (40623335)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2013: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
• Keywords: リン資源 / リン溶解菌 / 非晶質ケイ酸カルシウム / リンリサイクル / リン回収再資源化 / リサイクル / 微生物 / 土壌リン可溶化 / 製鋼スラグ / 培養 / 有機酸

Outline of Final Research Achievements

To recycle phosphate (Pi) that is immobilized in steelmaking slag and farm soils, bacterial strains capable of mobilizing Pi salts with calcium, iron or aluminum, were isolated from steelmaking slag and soil samples. Organic acids, including gluconic acid, citric acid, and acetic acid, which were extracellularly released from the isolated strains, were responsible for releasing phosphate from insoluble Pi salts. A simple, new technology for phosphate recovery was developed using amorphous calcium silicate hydrates which were chemically synthesized using unlimitedly available inexpensive materials such as calcium and silicates. The technologies developed in this study could potentially be used for recycling Pi immobilized in steelmaking slag and farm soils.

Research Products

• [Journal Article] A simple technology for phosphorus recovery using acid-treated concrete sludge (2016)
  • Author(s): Okano, K., Yamamoto, Y., Takano, H., Aketo, T., Honda, K., and Ohtake, H.
  • Journal Title: Separation and Purification Technology Volume: 165 Pages: 173-178
  • DOI: 10.1016/j.seppur.2016.03.054
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Amorphous calcium silicate hydrates and their possible mechanism (2015)
  • Author(s): Okano, K., Miyamaru, S., Kitao, A., Takano, T., Aketo, T., Toda, M., Honda, K., and Ohtake, H.
  • Journal Title: Separation and Purification Technology Volume: 144 Pages: 63-69
  • DOI: 10.1016/j.seppur.2015.01.043
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Development and implementation of technologies for recycling phosphorus in secondary resources in Japan (2015)
  • Author(s): Ohtake, H. and Okano, K.
  • Journal Title: Ｇｌｏｂａｌ Ｅｎｖｉｒｏｎｍｅｎｔａｌ Ｒｅｓｅａｒｃｈ Volume: 19 Pages: 1-15
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Greening the global phosphorus cycle (2015)
  • Author(s): Paul J.,Withers, A., Elser, J.J., Hilton, J., Ohtake, H., Schippere, W., and van Dijk, K.C.
  • Journal Title: Ｇｒｅｅn Chemistry Volume: 13 Issue: 4 Pages: 1-13
  • DOI: 10.1039/c4gc02445a
  • Peer Reviewed / Open Access
• [Journal Article] Novel technique for recovering phosphorus from aqueous solutions using (2013)
  • Author(s): Okano, K., Uemoto, M., Kagami, J., Miura, K., Aketo, T., Toda, M., Honda, K., and Ohtake, H.
  • Journal Title: Water Research Volume: 47 Issue: 7 Pages: 2251-2259
  • DOI: 10.1016/j.watres.2013.01.052
  • Peer Reviewed
• [Presentation] Enterobacter属細菌におけるリン酸欠乏誘導性のリン酸鉄溶解機構の解明 (2016)
  • Author(s): 山栗瑞希、矢野淳士、岡野憲司、本田孝祐、大竹久夫
  • Organizer: 日本農芸化学会2016年度大会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2016-03-29
• [Presentation] リン回収材としての非晶質ケイ酸カルシウムの可能性 (2016)
  • Author(s): 岡野憲司
  • Organizer: 第18回早稲田大学リンアトラス研究所セミナー
  • Place of Presentation: 早稲田大学
  • Year and Date: 2016-02-09
  • Invited
• [Presentation] Innovatively Simple, Low-Cost Technology for P Recovery Using Amorphous Calcium Silicate Hydrates (2015)
  • Author(s): Hisao Ohtake1, Kenji Okano, Masashi Kunisada, Hiroyuki Takano, and Toshio Matsuda
  • Organizer: 2nd European Sustainable Phosphorus Conference
  • Place of Presentation: Berlin, Germany
  • Year and Date: 2015-03-05 – 2015-03-06
• [Presentation] Development and Implementation of Technologies for Recycling Phosphorus in Secondary Resources in Japan (2015)
  • Author(s): Hisao Ohtake
  • Organizer: ScienceCampus P Research Rostock
  • Place of Presentation: Rostock, Germany
  • Year and Date: 2015-03-03
  • Invited