| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2015: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2014: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
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| Outline of Final Research Achievements |
This study was conducted aiming the construction of platelet production in vitro. First, we clarified that both intracellular oxidative stress and polyploidy inducers were important for the promotion of megakaryocytic differentiation. However, platelets generated from CHRF cells did not show the aggregating response due to the malfunction of GP1B protein. Next, the gp1b gene was introduced into CHRF cell and a stable cell line was successfully constructed. Finally, though the mRNA expression of gp1b gene increased 500 times comparing to the original CHRF cells, the expression of GP1B protein could not be confirmed.
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