Medical technological study of nano chemotherapy using hybrid liposomes for the refractory disease

• Project/Area Number: 25289299
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Sojo University
• Principal Investigator: UEOKA Ryuichi 崇城大学, 生物生命学部, 客員研究員 (70099076)
• Co-Investigator(Kenkyū-buntansha): MATSUMOTO Yoko 崇城大学, 生物生命学部, 教授 (00133562) ; MATSUSHITA Taku 崇城大学, 生物生命学部, 教授 (10209538) ; GOTO Koichi 崇城大学, 生物生命学部, 教授 (30279377) ; ICHIHARA Hideaki 崇城大学, 生物生命学部, 准教授 (70369114) ; KOMIZU Yuji 崇城大学, 生物生命学部, 助教 (80735829)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: リポソーム / 癌 / エイズ / 細胞膜 / アポトーシス / 揺らぎ / 細胞・組織 / セラミド

Outline of Final Research Achievements

We have produced hybrid liposomes(HL)which can be prepared by sonication of vesicular and micellar molecules in a buffer solution.We investigated the anticancer and anti-AIDS effects of HL and following results are obtained.(A)The remarkable inhibitory effects of HL on the growth of various tumor cells were attained in vitro.(B)apoptotic induction pathway which targeted tumor cell membranes was observed.(C)Significantly chemotherapeutic effects were obtained using mice model of carcinoma after the treatment with HL in vivo. Long-term selective accumulation of fluorescent labeled HL into tum or was observed in pharmacokinetics test using mice model of carcinoma. (D)therapetutic effects of treatment for the lymphoma with relation to AIDS was obtained.These findings suggest that HL should be a new type of nanomedicinal drug anti-cancer and anti-AIDS that targets refractory disease cell-membranes to trigger apoptotic cell death for various cancer cells.

Research Products

• [Journal Article] Targeting ceramide synthase 6-dependent metastasis-prone phenotype in lung cancer cells. (2016)
  • Author(s): Suzuki M, et al.
  • Journal Title: J. Clin. Invest. Volume: 126 Issue: 1 Pages: 254-265
  • DOI: 10.1172/jci79775
  • Peer Reviewed / Open Access
• [Journal Article] Therapeutic effects of hybrid liposomes with downregulation of inflammatory cytokine for model mice of rheumatoid arthritis in vivo (2015)
  • Author(s): Hideaki Ichihara, Shuichi Yamasaki, Motoki Hino, Yoko Matsumoto, Ryuichi Ueoka
  • Journal Title: Bioorganic & Medicinal Chemistry Letters Volume: 25 Issue: 13 Pages: 2686-2689
  • DOI: 10.1016/j.bmcl.2015.04.083
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Inhibitory effects of hybrid liposomes on the growth of non-small cell lung carcinoma cells and anti-invasive activity by ceramide generation without any drugs (2015)
  • Author(s): Hideaki Ichihara, Yuji Komizu, Ryuichi Ueoka, Yoko Matsumoto
  • Journal Title: Journal of Carcinogenesis & Mutagenesis Volume: 6 Issue: 04
  • DOI: 10.4172/2157-2518.1000230
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Hybrid liposomes inhibit the growth and angiogenesis in human breast cancer model (2015)
  • Author(s): Hideaki Ichihara, Shuichi Yamasaki, Motoki Hino, Ryuichi Ueoka, Yoko Matsumoto
  • Journal Title: Journal of Carcinogenesis & Mutagenesis Volume: 6 Issue: 01
  • DOI: 10.4172/2157-2518.1000207
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Therapeutic effects of hybrid liposomes with downregulation of inflammatory cytokine for model mice of rheumatoid arthritis in vivo (2015)
  • Author(s): Hideaki Ichihara, Shuichi Yamasaki, Motoki Hino, Ryuichi Ueoka, Yoko Matsumoto
  • Journal Title: Bioorganic Medicinal Chemistry Letters Volume: 印刷中
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Inhibition of HIV-1 entry by the tricyclic coumarin GUT-70 through the modification of membrane fluidity. (2015)
  • Author(s): Matsuda K, Hattori S, Kariya R, Komizu Y, Kudo E, Goto H, Taura M, Ueoka R, Kimura S, Okada S.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 457(3) Issue: 3 Pages: 288-94
  • DOI: 10.1016/j.bbrc.2014.12.102
  • Peer Reviewed / Open Access
• [Journal Article] Therapeutic effects of hybrid liposomes without drugs for rheumatoid arthritis (2014)
  • Author(s): Yoko Matsumoto, Hideaki Ichihara, Motoki Hino, Masayo Umebayashi, Ryuichi Ueoka
  • Journal Title: Drug Delivery Volume: 21 Issue: 5 Pages: 619-626
  • DOI: 10.3109/10717544.2013.869635
  • Peer Reviewed / Open Access
• [Journal Article] Cell cycle arrest by hybrid liposomes for human lung carcinoma cells (2014)
  • Author(s): Y. Komizu, M. Yukihara, Y. Matsumoto, R. Ueoka
  • Journal Title: J. Carcinog. Mutagen Volume: 5 Issue: 01
  • DOI: 10.4172/2157-2518.1000157
  • Peer Reviewed / Open Access
• [Journal Article] Cepharanthine inhibited HIV-1 cell-cell transmission and cell-free infection via modification of cell membrane fluidity (2014)
  • Author(s): K. Matsuda, S. Hattori, Y. Komizu, R. Kariya, R. Ueoka, S. Okada
  • Journal Title: Bioorg. Med. Chem. Lett. Volume: 24 Issue: 9 Pages: 2115-2117
  • DOI: 10.1016/j.bmcl.2014.03.041
  • Peer Reviewed / Open Access
• [Journal Article] Nanotherapy with hybrid liposomes for colorectal cancer along with apoptosis in vitro and in vivo (2014)
  • Author(s): Hideaki Ichihara, Shinichiro Nakagawa, Yusuke Matsuoka, Kenshi Yoshida, Yoko Matsumoto, Ryuichi Ueoka
  • Journal Title: Anticancer Research Volume: 34 Pages: 4701-4708
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Hybrid nanoparticles inhibit growth of human prostate cancer cells by induction of apoptosis (2014)
  • Author(s): Yuji Komizu, Toshiko Ueoka, Mamiko Yukihara, Hideaki Ichihara, Yoko Matsumoto, Ryuichi Ueoka
  • Journal Title: SOJ Pharmacy & Pharmaceutical Sciences Volume: 1 Pages: 1-4
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Therapeutic Effects of Cationic Hybrid Liposomes on the Hepatic Metastasis of Colon Carcinoma Along with Apoptosis <i>in Vivo</i> (2014)
  • Author(s): H. Ichihara, M. Hino, R. Ueoka, Y. Matsumoto
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 37 Issue: 3 Pages: 498-503
  • DOI: 10.1248/bpb.b13-00764
  • NAID: 130003390947
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed
• [Journal Article] ハイブリッドリポソームによるがん治療と分子メカニズム (2014)
  • Author(s): 上岡龍一, 市原英明, 古水雄志
  • Journal Title: 生物物理 Volume: 54 Pages: 5-10
  • NAID: 130003391392
• [Journal Article] membranes of normal murine thymocytes and leukemic GRSL cells (2013)
  • Author(s): Yoshimichi Andoha, Susumu Okazakia, Ryuichi Ueoka
  • Journal Title: Biochimica et Biophysica Acta (BBA) - Biomembranes Volume: 1828 Issue: 4 Pages: 1259-1270
  • DOI: 10.1016/j.bbamem.2013.01.005
  • Peer Reviewed
• [Journal Article] Therapeutic Effects of Autologous Lymphocytes Activated with Trastuzumab for Xenograft Mouse Models of Human Breast Cancer (2013)
  • Author(s): S. Nakagawa, Y. Matsuoka, H. Ichihara, H. Yoshida, K. Yoshida, R. Ueoka
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 36 Issue: 5 Pages: 861-865
  • DOI: 10.1248/bpb.b12-01084
  • NAID: 130003361416
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed
• [Journal Article] Immunostimulatory Effects of Extract from <i>Nori</i> (<i>Porphyra yezoensis</i>) <i>in Vitro</i> and <i>in Vivo</i> (2013)
  • Author(s): 北島 英樹, 古水 雄志, 市原 英明, 後藤 浩一, 土井 孝史, 稲野 達郎, 上岡 龍一
  • Journal Title: KAGAKU KOGAKU RONBUNSHU Volume: 39 Issue: 4 Pages: 359-362
  • DOI: 10.1252/kakoronbunshu.39.359
  • NAID: 10031188074
  • ISSN: 0386-216X, 1349-9203
  • Peer Reviewed
• [Journal Article] Hybrid liposomes inhibit tumor growth and lung metastasis of murine osteosarcoma cells (2013)
  • Author(s): H. Kitajima, Y. Komizu, H. Ichihara, K. Goto, R. Ueoka
  • Journal Title: Cancer Med Volume: (in press) Issue: 3 Pages: 267-278
  • DOI: 10.1002/cam4.67
  • Peer Reviewed
• [Journal Article] Novel Liposomes Composed of Dimyristoylphosphatidylcholine and Trehalose Surfactants Inhibit the Growth of Tumor Cells along with Apoptosis (2013)
  • Author(s): Y. Matsumoto, E. Cao, R. Ueoka
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 36 Issue: 8 Pages: 1258-1262
  • DOI: 10.1248/bpb.b13-00266
  • NAID: 130003361509
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed
• [Journal Article] Growth inhibition by novel liposomes including trehalose surfactant against hepatocarcinoma cells along with apoptosis (2013)
  • Author(s): Yoko Matsumoto, E. Cao, Ryuichi Ueoka
  • Journal Title: Anticancer Research Volume: 33 Pages: 4727-4740
  • Peer Reviewed
• [Presentation] Targeting CERS6-dependent sphingolipid homeostasis in lung cancer cells (2015)
  • Author(s): Motoshi Suzuki, Takashi Murate, Mamoru Kyogashima, Yuji Komizu, Yoko Matsumoto, Ryuichi Ueoka, Jin-ichi Inokuchi, Takashi Takahashi
  • Organizer: The 74nd Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: 名古屋国際会議場（愛知県 名古屋市）
  • Year and Date: 2015-10-08
• [Presentation] Therapeutic Effects of Hybrid Liposomes with Anti-angiogenic Activity for Breast Tumors (2014)
  • Author(s): Hideaki Ichihara, Motoki Hino, Ryuichi Ueoka, Yoko Matsumoto
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市西区みなとみらい）
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 熱力学量測定によるハイブリッドリポソームの揺らぎ検出 (2014)
  • Author(s): 中曽根祐介、古水雄志、松本陽子、松下琢、上岡龍一、寺嶋正秀
  • Organizer: 第8回分子科学討論会
  • Place of Presentation: 広島大学東広島キャンパス（広島県東広島市鏡山）
  • Year and Date: 2014-09-21 – 2014-09-24
• [Presentation] Chemotherapy and detection of cancer using hybrid liposomes (2014)
  • Author(s): Hideaki Ichihara, Yoko Matsumoto, Ryuichi Ueoka
  • Organizer: 9th International Forum on Multimedia and Image Processing (IFMIP) 2014 (World Automation Congress; WAC)
  • Place of Presentation: Hawaii（USA）
  • Year and Date: 2014-08-03 – 2014-08-07
  • Invited
• [Presentation] ハイブリッドリポソームの血管新生抑制活性による乳がん治療効果 (2014)
  • Author(s): 市原英明, 日野元貴, 松本陽子, 上岡龍一
  • Organizer: 第18回日本がん分子標的治療学会学術集会
  • Place of Presentation: 仙台市情報・産業プラザ（宮城県仙台市青葉区中央）
  • Year and Date: 2014-06-25 – 2014-06-27
• [Presentation] ハイブリッドリポソームを用いた形質転換肝幹細胞の選択的排除 (2014)
  • Author(s): 稲村恒亮, 石井貴晃, 押方歩, 古水雄志, 上岡龍一, 松下琢
  • Organizer: 日本組織培養学会 第 87 回大会
  • Place of Presentation: 星陵会館（東京都千代田区永田町）
  • Year and Date: 2014-05-29 – 2014-05-30
• [Presentation] ハイブリッドリポソームの肺がん細胞に対する細胞周期停止およびアポトーシス誘導によるがん抑制機構 (2013)
  • Author(s): 古水雄志, 松本陽子, 上岡龍一
  • Organizer: 第18回日本がん分子標的治療学会学術集会
  • Place of Presentation: 国立京都国際会館（京都市左京区）
• [Presentation] Cell cycle arrest by adding hybrid liposomes for lung tumor (2013)
  • Author(s): Yoko Matsumoto, Yuji Komizu, Mamiko Yukihara, Ryuichi Ueoka
  • Organizer: The 72nd Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: パシフィコ横浜（横浜市西区）
• [Presentation] Therapeutic effects of hybrid liposomes for mouse models of adult T-cell leukemia/lymphoma in vitro and in vivo (2013)
  • Author(s): Yuji Komizu, Hideaki Ichihara, Yoko Matsumoto, Ryuichi Ueoka
  • Organizer: The 72nd Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: パシフィコ横浜（横浜市西区）
• [Presentation] Chemotherapy with hybrid liposomes for acute lymphatic leukemia leading to apoptosis in vivo (2013)
  • Author(s): Hideaki Ichihara, Yoko Matsumoto, Ryuichi Ueoka
  • Organizer: The 72nd Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: パシフィコ横浜（横浜市西区）
• [Presentation] Immunostimulation effects of extract from nori (Porphyra yezoensis) in vitro and in vivo (2013)
  • Author(s): Koichi Goto, Hideki Kitajima, Yuji Komizu, Hideaki Ichihara, Takashi Doi, Tatsuro Inano, Ryuichi Ueoka
  • Organizer: The 72nd Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: パシフィコ横浜（横浜市西区）
• [Book] Membrane-Targeted Nanotherapy with Hybrid Liposomes for Cancer Cells Leading to Apoptosis, Molecular Science of Fluctuations Toward Biological Functions (2016)
  • Author(s): Ryuichi Ueoka, Yoko Matsumoto, Hideaki Ichihara, Yuji Komizu
  • Total Pages: 23
  • Publisher: Springer