Population coding of individual synaptic inputs

• Project/Area Number: 25290003
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Neurophysiology / General neuroscience
• Research Institution: University of Yamanashi (2014-2015); The University of Tokyo (2013)
• Principal Investigator: KITAMURA Kazuo 山梨大学, 総合研究部, 教授 (60423159)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2013: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
• Keywords: 神経情報処理 / シナプス結合 / 体性感覚 / 2光子イメージング / ２光子イメージング / 神経科学 / 生理学 / シナプス

Outline of Final Research Achievements

To elucidate the population coding by individual synaptic inputs, we have developed a method for visualizing the activity of single synapse in vivo by using two-photon imaging. We have shown that both spontaneous and sensory-evoked synaptic inputs are synchronized at neighboring synapses, and that spatiotemporal pattern of sensory-evoked synaptic inputs are similar to that of spontaneous inputs. Furthermore, we showed that these pattern of synaptic inputs are shaped by sensory experience during critical period. We also showed that the probability of coincident inputs in awake condition is much higher than that under anesthesia, indicating that the spatiotemporal pattern of synaptic inputs contributes to nonlinear dendritic integration under physiological conditions.

Research Products

• [Journal Article] Rational design of a high-affinity, fast, red calcium indicator R-CaMP2. (2015)
  • Author(s): Inoue M, Takeuchi A, Horigane S, Ohkura M, Gengyo-Ando K, Fujii H, Kamijo S, Takemoto-Kimura S, Kano M, Nakai J, Kitamura K, Bito H
  • Journal Title: Nat Methods Volume: 12 Issue: 1 Pages: 64-70
  • DOI: 10.1038/nmeth.3185
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Structure-Function Relationships between Aldolase C/Zebrin II Expression and Complex Spike Synchrony in the Cerebellum. (2015)
  • Author(s): Tsutsumi S, Yamazaki M, Miyazaki T, Watanabe M, Sakimura K, Kano M, Kitamura K
  • Journal Title: J Neurosci. Volume: 35(2) Issue: 2 Pages: 843-52
  • DOI: 10.1523/jneurosci.2170-14.2015
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Two distinct layer-specific dynamics of cortical ensembles during learning of a motor task. (2014)
  • Author(s): Masamizu Y, Tanaka YR, Tanaka YH, Hira R, Ohkubo F, Kitamura K, Isomura Y, Okada T, Matsuzaki M.
  • Journal Title: Nat. Neurosci Volume: 17 Issue: 7 Pages: 987-994
  • DOI: 10.1038/nn.3739
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Spike timing-dependent selective strengthening of single climbing fibre inputs to Purkinje cells during cerebellar development. (2013)
  • Author(s): Yoshinobu Kawamura, Hisako Nakayama, Kouichi Hashimoto, Kenji Sakimura, Kazuo Kitamura and Masanobu Kano.
  • Journal Title: Nat Commun. Volume: 4 Issue: 1 Pages: 2732-2732
  • DOI: 10.1038/ncomms3732
  • Peer Reviewed
• [Journal Article] Relationship between the local structure of orientation map and the strength of orientation tuning of neurons in monkey V1: a 2-photon calcium imaging study (2013)
  • Author(s): Ikezoe, K., Mori, Y., Kitamura, K., Tamura, H., Fujita, I.
  • Journal Title: Journal of Neuroscience Volume: 33(42) Issue: 42 Pages: 16818-16827
  • DOI: 10.1523/jneurosci.2209-13.2013
  • Peer Reviewed
• [Journal Article] Disruption of cerebellar microzonal organization in GluD2 (GluRd2) knockout mouse. (2013)
  • Author(s): Hashizume M, Miyazaki T, Sakimura K, Watanabe M, Kitamura K, Kano M
  • Journal Title: Front Neural Circuits Volume: 7 Pages: 130-130
  • DOI: 10.3389/fncir.2013.00130
  • Peer Reviewed
• [Journal Article] Functional labeling of neuronal ensemble and axonal projections in vivo using a synthetic activity-dependent promoter E-SARE. (2013)
  • Author(s): Kawashima T, Kitamura K, Suzuki K, Nonaka M, Kamijo S, Takemoto-Kimura S, Kano M, Okuno H, Ohki K, Bito H.
  • Journal Title: Nature Methods Volume: 10 Issue: 9 Pages: 889-895
  • DOI: 10.1038/nmeth.2559
  • Peer Reviewed
• [Journal Article] Nonlinear Decoding and Asymmetric Representation of Neuronal Input Information by CaMKIIa and Calcineurin. (2013)
  • Author(s): Fujii H, Inoue M, Okuno H, Sano Y; Takemoto-Kimura S, Kitamura K, Kano M, Bito H.
  • Journal Title: Cell Reports Volume: 3(印刷中)(掲載確定) Issue: 4 Pages: 978-987
  • DOI: 10.1016/j.celrep.2013.03.033
  • Peer Reviewed
• [Journal Article] Calcium-dependent regulation of climbing fiber synapse elimination during postnatal cerebellar development. (2013)
  • Author(s): Kano M, Nakayama H, Hashimoto K, Kitamura K, Sakimura K, Watanabe M
  • Journal Title: J. Physiol. Volume: 591 Issue: 13 Pages: 3151-3158
  • DOI: 10.1113/jphysiol.2012.248252
  • Peer Reviewed
• [Journal Article] Dendritic calcium signaling in cerebellar Purkinje cell. (2012)
  • Author(s): Kitamura K
  • Journal Title: Neural Networks Volume: - Pages: 11-7
  • DOI: 10.1016/j.neunet.2012.08.001
  • Peer Reviewed
• [Presentation] Functional organization of cerebellar modules during skilled behavior (2016)
  • Author(s): Kazuo Kitamura
  • Organizer: 第９３回日本生理学会大会
  • Place of Presentation: 札幌コンベンションセンター（北海道・札幌市）
  • Year and Date: 2016-03-22
• [Presentation] Optogenetic mapping of cerebra-cerebellar communication loop in mice (2016)
  • Author(s): Myeong Jeong Choo, Riichiro Hira, Masanori Matsuzaki, Masanobu Kano, Kazuo Kitamura
  • Organizer: 第９３回日本生理学会大会
  • Place of Presentation: 札幌コンベンションセンター（北海道・札幌市）
  • Year and Date: 2016-03-22
• [Presentation] Differential learning-related changes in climbing fiber inputs to cerebellar stripes (2016)
  • Author(s): Shinichiro Tsutsumi, Naoki Hidaka, Yoshikazu Isomura, Masanori Matsuzaki, Kenji Sakimura, Masanobu Kano, Kazuo Kitamura
  • Organizer: 第９３回日本生理学会大会
  • Place of Presentation: 札幌コンベンションセンター（北海道・札幌市）
  • Year and Date: 2016-03-22
• [Presentation] Rational design of ultrafast, high-affinity red calcium indicator for monitoring neuronal activity (2015)
  • Author(s): M. INOUE, A. TAKEUCHI, S.-I. HORIGANE, H. FUJII, S. KAMIJO, S. TAKEMOTO-KIMURA, M. OHKURA, K. GENGYO-ANDO, M. KANO, J. NAKAI, K. KITAMURA, H. BITO
  • Organizer: Neuroscience 2015
  • Place of Presentation: McCormick Place Convention Center (Chicago, USA)
  • Year and Date: 2015-10-17
  • Int'l Joint Research
• [Presentation] In vivo光学イメージングと電気生理学の融合 (2015)
  • Author(s): 喜多村和郎
  • Organizer: 第３８回日本神経科学大会
  • Place of Presentation: 神戸国際会議場（兵庫県・神戸市）
  • Year and Date: 2015-07-28
  • Invited
• [Presentation] Identification of complex spikes in two-photon recording of Purkinje cell calcium responses by machine learning and ROC analysis of the performance (2015)
  • Author(s): Huu Thien Hoang, Shinichiro Tsutsumi, Miki Hashizume, Okito Yamashita, Isao T. Tokuda, Masa-Aki Sato, Mitsuo Kawato, Masanobu Kano, Kazuo Kitamura, Keisuke Toyama
  • Organizer: 第３８回日本神経科学大会
  • Place of Presentation: 神戸国際会議場（兵庫県・神戸市）
  • Year and Date: 2015-07-28
• [Presentation] Rational design of a novel high-affinity, ultrafast, red calcium indicator R-CaMP2 (2015)
  • Author(s): Masatoshi Inoue, Atsuya Takeuchi, Shin-Ichiro Horigane, Masamichi Ohkura, Keiko Gengyo-Ando, Hajime Fujii, Satoshi Kamijo, Sayaka Takemoto-Kimura, Masanobu Kano, Junichi Nakai, Kazuo Kitamura, Haruhiko Bito
  • Organizer: 第３８回日本神経科学大会
  • Place of Presentation: 神戸国際会議場（兵庫県・神戸市）
  • Year and Date: 2015-07-28
• [Presentation] Experience-Dependent Clustering of Sensory Synaptic Inputs in the somatosensory cortex (2015)
  • Author(s): Kazuo Kitamura, Masanobu Kano
  • Organizer: 第９２回日本生理学会
  • Place of Presentation: 神戸国際会議場（兵庫県・神戸市）
  • Year and Date: 2015-03-23
• [Presentation] Two-photon imaging of neural activity at single neurons and single synapses: Optical physiology in vivo (2014)
  • Author(s): Kazuo Kitamura
  • Organizer: 第３７回日本神経科学学会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2014-09-12
• [Presentation] Highly sensitive calcium imaging of neural activity in vitro and in vivo with a new fluorescent calcium indicator, Cal-520 (2014)
  • Author(s): Atsuya Takeuchi, Mayumi Tada, Miki Hashizume, Kazuo Kitamura, Masanobu Kano
  • Organizer: 第３７回日本神経科学学会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2014-09-11
• [Presentation] Impairment of cerebellar microzonal organization by aberrant climbing fiber to Purkinje cell wiring in GluRδ2 (GluD2) knockout mouse. (2013)
  • Author(s): Miki Hashizume, Taisuke Miyazaki, Kenji Sakimura, Masahiko Watanabe, Kazuo Kitamura, Masanobu Kano
  • Organizer: Neuroscience2013
  • Place of Presentation: San Diego, USA
• [Presentation] Fine scale correspondence between the cerebellar microzones and the aldolase C compartments in mice. (2013)
  • Author(s): Shinichiro Tsutsumi, Maya Yamazaki, Taisuke Miyazaki, Masahiko Watanabe, Kenji Sakimura, Kazuo Kitamura, Masanobu Kano
  • Organizer: Neuroscience2013
  • Place of Presentation: San Diego, USA
• [Presentation] Selective strengthening of single climbing fiber inputs to Purkinje cells during postnatal cerebellar development in vivo. (2013)
  • Author(s): Yoshinobu Kawamura, Hisako Nakayama, Kouichi Hashimoto, Kenji Sakimura, Kazuo Kitamura, Masanobu Kano
  • Organizer: Neuroscience2013
  • Place of Presentation: San Diego, USA
• [Presentation] Postnatal desynchronization of climbing fiber responses in Purkinje cell populations of the developing cerebellum. (2013)
  • Author(s): Jean -M. Good, Taisuke Miyazaki, Kazuo Kitamura, Masahiko Watanabe, Kenji Sakimura, Masanobu Kano
  • Organizer: Neuroscience2013
  • Place of Presentation: San Diego, USA
• [Presentation] Synchronous climbing fiber responses correlated with the aldolase C compartments in mouse cerebellar cortex (2013)
  • Author(s): Shinichiro Tsutsumi, Mayumi Tada, Kenji Sakimura, Kazuo Kitamura, Masanobu Kano
  • Organizer: Neuro2013
  • Place of Presentation: 京都
• [Presentation] Sparse and heterogeneous organization of sensory inputs in layer 2/3 of mouse barrel cortex (2013)
  • Author(s): Kazuo Kitamura, Masanobu Kano
  • Organizer: Neuro2013
  • Place of Presentation: 京都
• [Presentation] 光で探る脳の活動 ～シナプスからニューロンまで～
  • Author(s): 喜多村和郎
  • Organizer: 第22回日本バイオイメージング学会学術集会公開講座
  • Place of Presentation: 東京
  • Invited
• [Presentation] 小脳皮質回路の機能構築と運動制御・運動記憶．
  • Author(s): 喜多村和郎
  • Organizer: 第2回記憶回路研究会
  • Place of Presentation: 岡崎
• [Presentation] 光で探る脳のはたらき～シナプスから回路へ～
  • Author(s): 喜多村和郎
  • Organizer: 量子エレクトロニクス研究会
  • Place of Presentation: 軽井沢