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Analysis of sleep-arousal regulation in Drosophila melanogaster

Research Project

Project/Area Number 25290007
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Neurophysiology / General neuroscience
Research InstitutionNagoya City University

Principal Investigator

KUME KAZUHIKO  名古屋市立大学, 薬学研究科(研究院), 教授 (30251218)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Keywords睡眠 / 覚醒 / ショウジョウバエ / ドーパミン / グルタミン酸受容体 / 学習記憶 / 概日周期 / 生物時計 / NMDA受容体
Outline of Final Research Achievements

We analyzed the sleep-arousal mechanism of a fruit fly, Drosophila melanogaster, which has been noted as a model for sleep study. First, we identified NMDA glutamate receptor as a novel sleep regulating gene. This provides a clue to the relationship between sleep and memory. Next we found that artificial sweeteners, but not non-sweet nutritive saccharides, induced sleep in fasting flies. Activation of sweet-receptor expressing neurons also induced sleep. Arousal threshold, however, was not reversed in artificial sweetener-fed flies. These results suggests that sweetness facilitates the transition from arousal to sleep, while the nutritional state, i.e. satiety inhibits that from sleep to arousal. In addition, we found glycine induces sleep in flies similarly in mammals, re-confirming that fruit fly can serve as a good model animal for sleep study.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (12 results)

All 2015 2014 2013 Other

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 3 results,  Acknowledgement Compliant: 4 results) Presentation (7 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results)

  • [Journal Article] The nutrient-responsive hormone CCHamide-2 controls growth by regulating insulin-like peptides in the brain of Drosophila melanogaster2015

    • Author(s)
      Sano H, Nakamur A, Texada M, Truma J, Ishimoto H, Kamikouchi A, Nibu Y, Kume K, Ida T, Kojima M
    • Journal Title

      PLoS Genetics

      Volume: 11 Issue: 5 Pages: 1-26

    • DOI

      10.1371/journal.pgen.1005209

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The NMDA receptor promotes sleep in the fruit fly, Drosophila melanogaster.2015

    • Author(s)
      Tomita, J., Ueno, T., Mitsuyoshi, M., Kume, S. and Kume, K.
    • Journal Title

      PLoS ONE

      Volume: 10 Issue: 5 Pages: e0128101-e0128101

    • DOI

      10.1371/journal.pone.0128101

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] New susceptibility variants to narcolepsy identified in HLA class II region.2015

    • Author(s)
      Miyagawa T, Toyoda H, Hirataka A, Kanbayashi T, Imanishi A, Honda M, Tokunaga K, et al.
    • Journal Title

      Hum. Mol. Genet.

      Volume: 24 Issue: 3 Pages: 891-898

    • DOI

      10.1093/hmg/ddu480

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Functional characterization of dopamine transporter in vivo using Drosophila melanogaster behavioral assays2014

    • Author(s)
      Ueno T, Kume K
    • Journal Title

      Frontiers in Behavioral Neuroscience

      Volume: 8 Pages: 303-303

    • DOI

      10.3389/fnbeh.2014.00303

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Temporal organization of rest defined by actigraphy data in healthy and childhood chronic fatigue syndrome children2013

    • Author(s)
      Minako Kawabata, Taro Ueno, Jun Tomita, Junko Kawatani, Akemi Tomoda, Shoen Kume and Kazuhiko Kume
    • Journal Title

      BMC Psychiatry

      Volume: 13 Issue: 1 Pages: 281-210

    • DOI

      10.1186/1471-244x-13-281

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] Dietary control of sleep and arousal threshold in Drosophila melanogaster2015

    • Author(s)
      Tatsuya Hasegawa, Jun Tomita, Kazuihko Kume
    • Organizer
      Cold Spring Harbor Laboratory Meeting
    • Place of Presentation
      New York, USA
    • Year and Date
      2015-10-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Regulation of sleep by dietary amino acids in Drosophila2015

    • Author(s)
      Shin Nakane, Jun Tomita, Kazuihko Kume
    • Organizer
      Cold Spring Harbor Laboratory Meeting
    • Place of Presentation
      New York, USA
    • Year and Date
      2015-10-03
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Neuronal amino acid transporter regulates sleep in Drosophila2015

    • Author(s)
      Jun Tomita, Taro Ueno, Kazuihko Kume
    • Organizer
      Cold Spring Harbor Laboratory Meeting
    • Place of Presentation
      New York, USA
    • Year and Date
      2015-10-02
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Signaling molecules involved in sleep regulation in Drosophila melanogaster2015

    • Author(s)
      Kazuhiko Kume
    • Organizer
      Asian Forum on Chronobiology in 2015
    • Place of Presentation
      Sapporo, Japan
    • Year and Date
      2015-09-08
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Large-scale objective sleep-data analysis using a contactless sleep monitor

    • Author(s)
      Kazuhiko Kume
    • Organizer
      XIII Congress of the European Biological Rhythms Society
    • Place of Presentation
      Ludwig Maximilians University, Munich, Germany
    • Related Report
      2013 Annual Research Report
  • [Presentation] Dopaminergic sleep and arousal regulation in Drosophila

    • Author(s)
      Taro Ueno, Jun Tomita, Shoen Kume, Kazuhiko Kume
    • Organizer
      CSHL Neurobiology of Drosophila Meeting
    • Place of Presentation
      Cold Spring Harbor Laboratory, NY, U.S.A.
    • Related Report
      2013 Annual Research Report
  • [Presentation] Male-male interaction-induced sleep in a fruit fly, Drosophila melanogaster

    • Author(s)
      Jun Tomita, Taro Ueno, Erina Sakamoto, Shoen Kume, Kazuhiko Kume
    • Organizer
      CSHL Neurobiology of Drosophila Meeting
    • Place of Presentation
      Cold Spring Harbor Laboratory, NY, U.S.A.
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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