| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
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| Outline of Final Research Achievements |
Using rat neuropathic pain models, we found the following findings.
1. PGD2 produced by microglia is COX-1 dependent, and neurons in the spinal cord can receive PGD2 from microglia following peripheral nerve injury, suggesting a role of PGD2 signaling in mechanical allodynia. 2. We found the details of RhoA/ROCK pathway in spinal microglia in this model. It mediates p38 MAPK activation and morphological changes downstream of P2Y12/13 receptors in spinal microglia in neuropathic pain. 3. We found that increased artemin in peripheral tissues plays an important role on the regulation of TRPV1/A1 in DRG neurons in pathological conditions such as inflammatory and neuropathic pain. 4. Using electrophysiological method, we found leukotriene enhances NMDA-induced inward currents in dorsal horn neurons of the rat spinal cord after peripheral nerve injury. 5. We found that microglial TNF alpha is able to induce COX2 and PGI2 synthase expression in spinal endothelial cells during neuropathic pain.
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