Study on molecular mechanism of cerebellar circuit formation
Project/Area Number |
25290021
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Neurochemistry/Neuropharmacology
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Research Institution | Shinshu University |
Principal Investigator |
UEMURA Takeshi 信州大学, 学術研究院医学系, 准教授 (00372368)
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Co-Investigator(Renkei-kenkyūsha) |
YOSHIDA Tomoyuki 富山大学, 医学薬学研究院, 准教授 (90372367)
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Project Period (FY) |
2013-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000)
Fiscal Year 2015: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2013: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
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Keywords | 脳・神経 / 小脳 / 神経回路網構築 / 神経回路網形成 |
Outline of Final Research Achievements |
Synapses are formed and maintained by trans-synaptic interaction between pre- and postsynaptic cell adhesion molecules. In the cerebellum, the trans-synaptic interaction of postsynaptic glutamate receptor GluRδ2 and presynaptic neurexins (Nrxns) through cerebellin precursor protein 1 (Cbln1) mediates parallel fiber (PF)-Purkinje cell (PC) synapse formation. In mammals, Nrxns are encoded by three genes (Nrxn1, Nrxn2, and Nrxn3). In this study, we generated cerebellar granule cell (GC)-specific Nrxn1, Nrxn2, and Nrxn3 triple KO mice. The cerebellar GCs-specific Nrxns triple KO mice showed severe ataxia and reduced cerebellar size. Histological analysis revealed that the number of cerebellar GCs was dramatically decreased in the cerebellar GCs-specific Nrxns triple KO mice. Electron microscopic analysis showed that PF-PC synapses were disappeared in the GCs-specific Nrxns triple KO mice. These results suggest that Nrxns are essential for cerebellar circuit formation.
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Report
(5 results)
Research Products
(22 results)
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[Journal Article] Fluorescent protein tagging of endogenous protein in brain neurons using CRISPR/Cas9-mediated knock-in and in utero electroporation techniques.2016
Author(s)
Uemura T, Mori T, Kurihara T, Kawase S, Koike R, Satoga M, Cao X, Li X, Yanagawa T, Sakurai T, Shindo T, Tabuchi K.
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Journal Title
Sci Rep
Volume: 6
Issue: 1
Pages: 35861-35861
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Mechanisms of splicing-dependent trans-synaptic adhesion by PTPδ-IL1RAPL1/IL-1RAcP for synaptic differentiation2015
Author(s)
Yamagata A., Yoshida, T., Sato, Y., Goto-Ito, S., Uemura, T., Maeda, A., Shiroshima, T., Iwasawa-Okamoto, S., Mori, H., Mishina, M., Fukai , S.
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Journal Title
Nature Communications
Volume: 6
Issue: 1
Pages: 6926-6926
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] IL1RAPL1 knockout mice show learning deficiency, memory decline, hyperactivity and reduced anxiety-like behaviours.2014
Author(s)
Misato Yasumura, Tomoyuki Yoshida, Maya Yamazaki, Manabu Abe, Rie Natsume, Kouta Kanno, Takeshi Uemura, Keizo Takao, Kenji Sakimura, Takefumi Kikusui, Tsuyoshi Miyakawa, Masayoshi Mishina
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Journal Title
Scientific Reports
Volume: 4: 6613
Issue: 1
Pages: 6613-6613
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Structural basis of IL1RAPL1-PTPδ complex for neuronal synaptogenesis2015
Author(s)
Tomoyuki Yoshida, Atsushi Yamagata, Yusuke Sato, Sakurako Ito:, Takeshi Uemura, Asami Maeda, Tomoko Shiroshima, Shiho Okamoto, Hisashi Mori, Masayoshi Mishina, Shuya Fukai
Organizer
Neuro2015
Place of Presentation
兵庫県神戸市 「神戸国際会議場」
Year and Date
2015-07-30
Related Report
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[Presentation] The Atmospheric Scanning Electron Microscope (ASEM) Observes Axonal Segmentation and Synaptic Induction in Solution2014
Author(s)
2.佐藤 主税, 木下 貴明, 植村 健, 平野 和己, 川田 正晃, 西山英利, 佐藤 真理, 海老原 達彦, 須賀三雄, 西原祥子
Organizer
2014 Microscopy & Microanalysis meeting
Place of Presentation
ハートフォード, アメリカ合衆国
Year and Date
2014-08-05
Related Report
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[Presentation] IL1RAPL1欠損マウスの行動学的解析2013
Author(s)
安村美里, 吉田知之, 高雄啓三, 山崎真弥, 阿部学, 植村健, 宮川剛, 崎村建司, 三品昌美
Organizer
第36回日本神経科学大会
Place of Presentation
国立京都国際会館(京都府京都市)
Year and Date
2013-06-21
Related Report
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