Physiology and pathology of endocytosis and recycling transport in neurons
Project/Area Number |
25290024
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Neurochemistry/Neuropharmacology
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Research Institution | Tokyo Metropolitan University |
Principal Investigator |
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Co-Investigator(Renkei-kenkyūsha) |
SAITO TARO 首都大学東京, 大学院理工学研究科, 助教 (70301413)
ASADA AKIKO 首都大学東京, 大学院理工学研究科, 助教 (00336512)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2013: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
|
Keywords | タンパク質 / 酵素 / 神経科学 / 発生・分化 / Cdk5 / LMTK1 / Rab / GRAB / Grab / 老化 |
Outline of Final Research Achievements |
Cdk5 is a brain specific membrane-bound protein kinase. We have analyzed a role of Cdk5 in neurite outgrowth in mouse primary neurons. Cdk5 phosphorylates LMTK1, a novel Ser/Thr kinase in brains, and GRAB, an activator of Rab8. Phosphorylated LMTK1 suppressed activation of Rab11 and phosphorylated GRAB lost the Rab9 activation activity. Rab11 and Rab8 are major regulators in endosome transport, supplying membrane components to the tip of axon or dendrites. Thus, their inactivation results in the reduced neurite outgrowth. This signaling cascade is negative regulatory mechanism of neurite outgrowth.
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Report
(4 results)
Research Products
(37 results)
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[Journal Article] Preferential targeting of p39-activated Cdk5 to Rac1-induced lamellipodia.2014
Author(s)
Ito, Y., Asada, A., Kobayashi, H., Takano, T., Sharma, G., Saito, T., Ohta, Y., Amano, M., Kaibuchi, K., and Hisanaga, S.
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Journal Title
Molecular and Cellular Neurosciences
Volume: 61
Pages: 34-45
DOI
Related Report
Peer Reviewed
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[Journal Article] Cyclin-dependent kinase 5 phosphorylates and induces the degradation of ataxin-22014
Author(s)
Asada, A., Yamazaki, R., Kino, Y., Saito, T., Kimura, T., Miyake, M., Hasegawa, M., Nukina, N. & Hisanaga, S.
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Journal Title
Neurosci. Lett.
Volume: 563
Pages: 112-117
DOI
Related Report
Peer Reviewed
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[Journal Article] Isomerase Pin1 Stimulates Dephosphorylation of Tau Protein at Cyclin-dependent Kinase (Cdk5)-dependent Alzheimer Phosphorylation Sites2013
Author(s)
Taeko Kimura, Koji Tsutsumi, Masato Taoka, Taro Saito, Masami Masuda-Suzukake, Koichi Ishiguro, Florian Plattner, Takafumi Uchida, Toshiaki Isobe, Masato Hasegawa, and Shin-ichi Hisanaga
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Journal Title
THE JOURNAL OF BIOLOGICAL CHEMISTRY
Volume: 288
Issue: 11
Pages: 7968-7977
DOI
Related Report
Peer Reviewed
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[Journal Article] Dab1-mediated colocalization of multi-adaptor protein CIN85 with Reelin-receptors, ApoER2 or VLDLR, in neurons.2013
Author(s)
T.Fuchigami, Y.Sato1, Y.Tomita1, T.Takano, S.Miyauchi, Y.Tsuchiya, T.Saito, K.Kubo, K.Nakajima, M.Fukuda, M.Hattori, and S.Hisanaga
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Journal Title
Genes to Cells
Volume: in press
Issue: 5
Pages: 410-424
DOI
Related Report
Peer Reviewed
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