Understanding of mechanism underlying primordial follicle formation using a pluripotent stem cell-based model
Project/Area Number |
25290033
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Laboratory animal science
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Research Institution | Kyushu University (2014-2016) Kyoto University (2013) |
Principal Investigator |
HAYASHI Katsuhiko 九州大学, 医学(系)研究科(研究院), 教授 (20287486)
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Project Period (FY) |
2013-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
|
Keywords | 卵母細胞 / 多能性幹細胞 / 生殖細胞 / 原始卵胞 / 卵子 / 始原生殖細胞 / 体外培養 / 減数分裂 / 単一細胞遺伝子発現 / 次世代シークエンサー |
Outline of Final Research Achievements |
The aim of this research project is to establish a culture system that reproduce formation and maintenance of primordial follicles. This project is particularly unique in the point of using pluripotent stem cells, such as embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), as a starting material. Reconstitution of primordial follicle formation/maintenance from plruipotent stem cells would be quite beneficial, as genetic manipulations in the cells are quite feasible. The culture system in this project will contribute to understanding of mechanisms underlying primordial follicle formation/maintenance, which will eventually elucidate causes of infertility.
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Report
(5 results)
Research Products
(49 results)
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[Journal Article] Reconstitution in vitro of the entire cycle of the mouse female germ line.2016
Author(s)
Hikabe O, Hamazaki N, Nagamatsu G, Obata Y, Hirao Y, Hamada N, Shimamoto S, Imamura T, Nakashima K, Saitou M, Hayashi K
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Journal Title
Nature
Volume: 539
Issue: 7628
Pages: 299-303
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Rewinding the process of mammalian extinction.2016
Author(s)
Saragusty J, Diecke S, Drukker M, Durrant B, Friedrich B-N I, Galli C, Göritz F, Hayashi K, Hermes R, Holtze S, Johnson S, Lazzari G, Loi P, Loring JF, Okita K, Renfree MB, Seet S, Voracek T, Stejskal J, Ryder OA, Hildebrandt TB.
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Journal Title
Zoo Biol.
Volume: 35
Issue: 4
Pages: 280-292
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Quantitative Dynamics of Chromatin Remodeling during Germ Cell Specification from Mouse Embryonic Stem Cells2015
Author(s)
Kurimoto, K., Yabuta, Y., Hayashi, K., Ohta, H., Kiyonari, H., Mitani, T., Moritoki, Y., Kohri, K., Kimura, H., Yamamoto, T., Katou, Y., Shirahige, K., Saitou, M.
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Journal Title
Cell Stem Cell
Volume: 16
Issue: 5
Pages: 517-532
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Induction of Primordial Germ Cell-Like Cells From Mouse Embryonic Stem Cells by ERK Signal Inhibition.2014
Author(s)
Kimura T, Kaga Y, Ohta H, Odamoto M, Sekita Y, Li K, Yamano N, Fujikawa K, Isotani A, Sasaki N, Toyoda M, Hayashi K, Okabe M, Shinohara T, Saitou M, Nakano T.
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Journal Title
Stem Cells
Volume: 32
Issue: 10
Pages: 2668-2678
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] A mesodermal factor, T, specifies mouse germ cell fate by directly activating germline determinants2013
Author(s)
Aramaki, S., Hayashi, K., Kurimoto, K., Ohta, H., Yabuta, Y., Iwanari, H., Mochizuki, Y., Hamakubo, T., Kato, Y., Shirahige, K., and Saitou, M
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Journal Title
Developmental Cell
Volume: 27
Issue: 5
Pages: 516-529
DOI
Related Report
Peer Reviewed / Open Access
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