| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
|
|---|
| Outline of Final Research Achievements |
In order to inactivate, by insertional mutagenesis, all protein-coding genes in mouse undifferentiated embryonic-stem (ES) cells, the knockout mouse project (KOMP) had been carried out in the USA, Canada, UK, and Germany as a large-scale and international collaborative effort. In Japan, on the other hand, a gene-trapping strategy termed UPATrap, which we had originally developed, was further upgraded in the national bio-resource project (NBRP) of Monka-sho (MEXT). In the current research, we have tried to establish a novel methodology for the random disruption (trapping) of the transcriptionally silent genes of non-coding RNAs in the target cells.
|
