Development of a gene-trap strategy for the transcriptionally silent genes of non-coding RNAs in the target cells
| Project/Area Number |
25290034
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Laboratory animal science
|
|---|
| Research Institution | Nara Institute of Science and Technology |
|---|
Principal Investigator |
Ishida Yasumasa 奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (10221756)
|
|---|
| Project Period (FY) |
2013-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
|
|---|
| Keywords | 遺伝子トラップ / ＵＰＡＴｒａｐ / 非コードＲＮＡ遺伝子 / ＥＳ細胞 / マウス / ES細胞 / 長鎖非コードRNA / NMD / リサーチバイオリソース / ジフテリア毒素 / ノックアウトマウス |
|---|
| Outline of Final Research Achievements |
In order to inactivate, by insertional mutagenesis, all protein-coding genes in mouse undifferentiated embryonic-stem (ES) cells, the knockout mouse project (KOMP) had been carried out in the USA, Canada, UK, and Germany as a large-scale and international collaborative effort. In Japan, on the other hand, a gene-trapping strategy termed UPATrap, which we had originally developed, was further upgraded in the national bio-resource project (NBRP) of Monka-sho (MEXT). In the current research, we have tried to establish a novel methodology for the random disruption (trapping) of the transcriptionally silent genes of non-coding RNAs in the target cells.
|
Report
(5 results)
Research Products
(3 results)
