| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2015: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Outline of Final Research Achievements |
The Ay allele promoted pancreatic carcinogenesis in Ptf1a-KrasG12D mice and azoxymethane (AOM)-induced colorectal carcinogenesis in diabetic KK mice. Increased expression of CSF1 and accumulation of macrophages was observed in pancreatic carcinoma tissues in Ptf1a-KrasG12D-Ay mice and AOM-induced colorectal carcinoma tissues in KK-Ay mice. The agouti overexpression in pancreatic cancer cell lines established from Ptf1a-KrasG12D mice increases the expression of adipogenesis-related gene X via antagonizing MCRs. The gene X product significantly elevated CSF1 expression. Expression of the gene X was decreased by treatment with forskolin, which elevates intracellular cAMP and stimulates downstream pathways of MCRs. However, effects of forskolin on inflammatory factors were different among cell lines and the effects on carcinogenesis was supposed to be not consistent.
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