| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Outline of Final Research Achievements |
We analyzed the function of NOL11 that were identified as the factor that were involved in mitosis from large scale siRNA screening. NOL11 formed complex with WDR43 and Cirhin, and associated with periphery of mitotic chromosomes. We found that NOL11 KD caused mitotic defects such as aberrant mitotic chromosome alignment and a delay in mitotic progression. Delay in mitotic progression was induced by inhibition of cdc2 kinase due to increased inhibitory phosphorylation of cdc2 kinase at Tyr15. In addition to the mitotic phenotype, NOL11 KD reduced rRNA transcription and caused nucleolar disruption during interphase. Interestingly, delay in mitotic progression was also observed when nucleolar disruption during interphase was induced by other methods. Furthermore, the defects was ameliorated when nucleolar disruption during interphase was suppressed. Thus, nucleolar integrity during interphase is required for proper mitotic progression.
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