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Analysis of nucleolar RNA-protein network that regulates mitotic chromosome dynamics

Research Project

Project/Area Number 25290064
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Genome biology
Research InstitutionUniversity of Tsukuba

Principal Investigator

KIMURA Keiji  筑波大学, 生命環境系, 准教授 (50332268)

Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Keywords分裂期 / 染色体 / 核小体 / RNA / Aurora B / NOL11 / リン酸化 / RNA / NOL11
Outline of Final Research Achievements

We analyzed the function of NOL11 that were identified as the factor that were involved in mitosis from large scale siRNA screening. NOL11 formed complex with WDR43 and Cirhin, and associated with periphery of mitotic chromosomes. We found that NOL11 KD caused mitotic defects such as aberrant mitotic chromosome alignment and a delay in mitotic progression. Delay in mitotic progression was induced by inhibition of cdc2 kinase due to increased inhibitory phosphorylation of cdc2 kinase at Tyr15. In addition to the mitotic phenotype, NOL11 KD reduced rRNA transcription and caused nucleolar disruption during interphase. Interestingly, delay in mitotic progression was also observed when nucleolar disruption during interphase was induced by other methods. Furthermore, the defects was ameliorated when nucleolar disruption during interphase was suppressed. Thus, nucleolar integrity during interphase is required for proper mitotic progression.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (19 results)

All 2017 2016 2015 2014 2013 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 4 results,  Acknowledgement Compliant: 3 results) Presentation (12 results) Remarks (1 results)

  • [Journal Article] Gradual reduction in rRNA transcription triggers p53 acethlation and apoptosis via MYBBP1A2015

    • Author(s)
      Kumazawa T, Nishimura K, Katagiri N, Hashimoto S, Hayashi Y, and Kimura K
    • Journal Title

      Scientific Reports

      Volume: 5 Pages: 10854-10854

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The nucleolar protein nucleophosmin is essential for autophagy induced by inhibiting Pol I transcription2015

    • Author(s)
      Naohiro Katagiri, Takao Kuroda, Hiroyuki Kishimoto, Yuki Hayashi, Takuya Kumazawa, Keiji Kimura
    • Journal Title

      Scientific Reports

      Volume: 5 Issue: 1 Pages: 8903-8903

    • DOI

      10.1038/srep08903

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Perturbation of Ribosome Biogenesis Drives Cells into Senescence through 5S RNP-Mediated p53 Activation.2015

    • Author(s)
      Nishimura K, Kumazawa T, Kuroda T, Katagiri N, Tsuchiya M, Goto N, Furumai R, Murayama A, Yanagisawa J, Kimura K
    • Journal Title

      Cell Reports

      Volume: 10 Issue: 8 Pages: 1310-1323

    • DOI

      10.1016/j.celrep.2015.01.055

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Downregulation of rRNA transcription triggers cell differentiation.2014

    • Author(s)
      Hayashi Y, Kuroda T, Kishimoto H, Wang C, Iwama A, Kimura K.
    • Journal Title

      PLoS One

      Volume: 9 Issue: 5 Pages: e98586-e98586

    • DOI

      10.1371/journal.pone.0098586

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The Nucleolar Protein Myb-binding Protein 1A (MYBBP1A) Enhances p 53 Tetramerization and Acetylation in Response to Nucleolar Disruption2014

    • Author(s)
      Ono W, Hayashi Y, Yokoyama W , Kuroda T, Kishimoto H, Ito I, Kimura K, Akaogi K, Waku T, Yanagisawa J.
    • Journal Title

      Journal of Biological Chemistry

      Volume: 289 Issue: 8 Pages: 4928-4940

    • DOI

      10.1074/jbc.m113.474049

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nucleolar protein, Myb-binding protein 1A, specifically binds to nonacetylated p53 and efficiently promotes transcriptional activation2013

    • Author(s)
      Ono W, Akaogi K, Waku T, Kuroda T, Yokoyama W, Hayashi Y, Kimura K, Kishimoto H, Yanagisawa J
    • Journal Title

      Biochem. Biophys.Res.Commun

      Volume: 434 Pages: 659-663

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] 間期核小体構造と時空間的なCdk1活性制御2017

    • Author(s)
      林優樹、藤村亜紀子、加藤かざし、宇田川里奈、広田亨、木村圭志
    • Organizer
      第34回染色体ワークショップ 第15回核ダイナミクス研究会
    • Place of Presentation
      千葉県・木更津市・かずさアカデミアホール
    • Year and Date
      2017-01-12
    • Related Report
      2016 Annual Research Report
  • [Presentation] 核小体タンパク質NOL11はCdk1の活性化を調節する2016

    • Author(s)
      林優樹、藤村亜紀子、加藤かざし、宇田川里奈、広田亨、木村圭志
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      神奈川県・横浜市・パシフィコ横浜
    • Year and Date
      2016-12-02
    • Related Report
      2016 Annual Research Report
  • [Presentation] ホスファターゼによる分裂期染色体結合タンパク質の制御2016

    • Author(s)
      郡司理紗子、松井亮仁、林優樹、木村圭志
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      神奈川県・横浜市・パシフィコ横浜
    • Year and Date
      2016-12-02
    • Related Report
      2016 Annual Research Report
  • [Presentation] RNAによるM期染色体の構造と結合タンパク質の維持2016

    • Author(s)
      加藤かざし、小暮祐一郎、林優樹、木村圭志
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      神奈川県・横浜市・パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] rRNAプロセシング因子NOL11によるM期染色体動態の制御2016

    • Author(s)
      林 優樹、加藤 かざし、藤村 亜紀子、郡司 理紗子、松高 愛、広田 亨、木村 圭志
    • Organizer
      第33回染色体ワークショップ第14回核ダイナミクス研究会
    • Place of Presentation
      宮城県・松島・松島一の坊
    • Year and Date
      2016-01-12
    • Related Report
      2015 Annual Research Report
  • [Presentation] 核小体は各種ストレス時の細胞の運命を決める2015

    • Author(s)
      熊澤 拓也、西村 和帆、片桐 尚宏、竹田 征治、斎藤 能彦、木村 圭志
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      兵庫県・神戸・ポートアイランド
    • Year and Date
      2015-12-04
    • Related Report
      2015 Annual Research Report
  • [Presentation] 各小隊タンパク質Nucleophosminを介した新規オートファジー誘導経路の解析2015

    • Author(s)
      片桐 尚宏、林 優樹、熊澤 拓也、木村 圭志
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      兵庫県・神戸・ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 各種ストレス時における細胞の運命決定メカニズムの解析2014

    • Author(s)
      西村和帆,熊澤拓也,黒田貴雄,木村圭志
    • Organizer
      日本分子生物学会年会
    • Place of Presentation
      神奈川県・パシフィコ横浜
    • Year and Date
      2014-11-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] 核小体崩壊によって誘導されるオートファジーに、核小体タンパク質NPMが関与する2014

    • Author(s)
      片桐尚宏,林優樹,熊澤拓也,木村圭志
    • Organizer
      日本分子生物学会年会
    • Place of Presentation
      神奈川県・パシフィコ横浜
    • Year and Date
      2014-11-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] 核小体因子によるAurora Bを介したM期染色体動態の制御

    • Author(s)
      木村 圭志
    • Organizer
      第31回染色体ワークショップ第12核ダイナミクス研究会
    • Place of Presentation
      神奈川県・足柄郡・ホテルおかだ
    • Related Report
      2013 Annual Research Report
  • [Presentation] 核小体因子はAurora Bを介してM期染色体を制御する

    • Author(s)
      木村 圭志
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      兵庫県・神戸国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] 核小体因子によるAurora Bを介した M期染色体動態制御

    • Author(s)
      木村 圭志
    • Organizer
      蛋白研セミナー
    • Place of Presentation
      大阪府・大阪大学
    • Related Report
      2013 Annual Research Report
  • [Remarks] 筑波大学大学院 生体情報制御学研究室

    • URL

      http://www.agbi.tsukuba.ac.jp/~frog/

    • Related Report
      2016 Annual Research Report

URL: 

Published: 2013-05-21   Modified: 2019-07-29  

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