Analysis of nucleolar RNA-protein network that regulates mitotic chromosome dynamics
| Project/Area Number |
25290064
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
KIMURA Keiji 筑波大学, 生命環境系, 准教授 (50332268)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Keywords | 分裂期 / 染色体 / 核小体 / RNA / Aurora B / NOL11 / リン酸化 / ＲＮＡ / ＮＯＬ11 |
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| Outline of Final Research Achievements |
We analyzed the function of NOL11 that were identified as the factor that were involved in mitosis from large scale siRNA screening. NOL11 formed complex with WDR43 and Cirhin, and associated with periphery of mitotic chromosomes. We found that NOL11 KD caused mitotic defects such as aberrant mitotic chromosome alignment and a delay in mitotic progression. Delay in mitotic progression was induced by inhibition of cdc2 kinase due to increased inhibitory phosphorylation of cdc2 kinase at Tyr15. In addition to the mitotic phenotype, NOL11 KD reduced rRNA transcription and caused nucleolar disruption during interphase. Interestingly, delay in mitotic progression was also observed when nucleolar disruption during interphase was induced by other methods. Furthermore, the defects was ameliorated when nucleolar disruption during interphase was suppressed. Thus, nucleolar integrity during interphase is required for proper mitotic progression.
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Report
(5 results)
Research Products
(19 results)
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[Journal Article] Nucleolar protein, Myb-binding protein 1A, specifically binds to nonacetylated p53 and efficiently promotes transcriptional activation2013
Author(s)
Ono W, Akaogi K, Waku T, Kuroda T, Yokoyama W, Hayashi Y, Kimura K, Kishimoto H, Yanagisawa J
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Journal Title
Biochem. Biophys.Res.Commun
Volume: 434
Pages: 659-663
Related Report
Peer Reviewed
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