| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
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| Outline of Final Research Achievements |
There are two cohesin acetyl transferase, Esco1 and Esco2, in human. Both of them are essential for establishment of sister chromatids cohesion. We found that Esco1 directly binds to cohesin subunit Pds5 and co-localizes with cohesin. The domain for binding with Pds5 is responsible for cohesin acetylation and cohesion establishment. Esco1 phosphorylated in mitosis prevents its binding with Pds5. Therefore phosphorylation may have important role in controlling Esco1 localization. Esco2 associates with MCM helicases during G1 to S-phase. The N-terminal domain of Esco2 conserved among vertebrates is required for direct interation with MCM. It has been known that Esco2 is down-regulated from late G2 to M phase. Interestingly Esco2 mutant that is unable to bind to Mcm is destabilized. This destabilization of Esco2 is caused by proteaosome pathway mediated by Cul4 ligase. Thus, Esco1 and Esco2 function in different pathway from each other to promote cell cycle progression.
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