Regulation of microRNA genes by Sox transcription factors

• Project/Area Number: 25290066
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Genome biology
• Research Institution: Kochi University of Technology (2015-2016); Osaka University (2013-2014)
• Principal Investigator: Kamachi Yusuke 高知工科大学, 環境理工学部, 教授 (90263334)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000); Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2013: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
• Keywords: 転写因子 / microRNA / 遺伝子 / 発生・分化 / ゲノム

Outline of Final Research Achievements

A microRNA is a small non-coding RNA molecule that plays an important role in cell differentiation and cell proliferation. However, it remains largely unknown how expression of the microRNA gene family is controlled during embryonic development. In this study, we investigated regulation of the microRNA genes including the mir-17-92 cluster gene, which is indispensable for vertebrate embryogenesis and also known as cancer microRNA. We found that Sox transcription factors are involved in the regulation of several microRNA genes.

Research Products

• [Journal Article] Regulation of trunk neural crest delamination by δEF1 and Sip1 in the chicken embryo (2016)
  • Author(s): Yasumi, T., Inoue, M., Maruhashi, M., Kamachi, Y., Higashi, Y., Kondoh, H., Uchikawa, M.
  • Journal Title: Dev. Growth. Differ. Volume: 58 Issue: 2 Pages: 205-214
  • DOI: 10.1111/dgd.12256
  • Peer Reviewed
• [Journal Article] Sox proteins: regulators of cell fate specification and differentiation (2013)
  • Author(s): Kamachi Y, Kondoh H
  • Journal Title: Development Volume: 140 Issue: 20 Pages: 4129-4144
  • DOI: 10.1242/dev.091793
  • Peer Reviewed
• [Journal Article] Nano-analysis of DNA conformation changes induced by transcription factor complex binding using plasmonic nanodimers (2013)
  • Author(s): Morimura H, Tanaka S, Ishitobi H, Mikami T, Kamachi Y, Kondoh H, Inouye Y.
  • Journal Title: ACS Nano Volume: 7 Issue: 12 Pages: 10733-10740
  • DOI: 10.1021/nn403625s
  • Peer Reviewed
• [Presentation] Cooperative activation of Sall4 and Sox8 transcription factors in the regulation of the chicken Sox3 gene during inner ear development (2017)
  • Author(s): Okamoto, Y.,, Nishimura, N., Matsuda K., Ranawakage, D., Kamachi, Y., Kondoh, H., and Uchikawa, M.
  • Organizer: Annual Meeting of the Japanese Society of Developmental Biology
  • Place of Presentation: Tokyo
  • Year and Date: 2017-05-10
• [Presentation] ゼブラフィッシュの眼とヒレの発生におけるSox11転写因子の役割 (2014)
  • Author(s): 蒲池 雄介
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Molecular functions of Sox11 in zebrafish development: focus on retina and fin development (2014)
  • Author(s): Kamachi, Yusuke
  • Organizer: 4th International SOX Research Conference
  • Place of Presentation: Cleveland, USA
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] Sox11 に依存した miRNA-17-92 クラスター発現の制御機構
  • Author(s): 川合 杏奈, 近藤 寿人, 蒲池 雄介
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（兵庫県）
• [Book] Sox2: Biology and Role in Development and Disease. (Editors: Kondoh and Lovell-Badge) (2015)
  • Author(s): Kamachi, Y. (Chapter 6: Evolution of Sox2 and Functional Redundancy in Relation to Other SoxB1 Genes)
  • Total Pages: 344
  • Publisher: Elsevier
• [Book] Sox2: Biology and Role in Development and Disease. (Editors: Kondoh and Lovell-Badge) (2015)
  • Author(s): kondoh, H. and Kamachi, Y. (Chapter 8: SOX2-Partner Factor Interactions and Enhancer Regulation)
  • Total Pages: 344
  • Publisher: Elsevier