| Project/Area Number |
25290072
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical genome science
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| Research Institution | Japan Advanced Institute of Science and Technology |
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Principal Investigator |
Tsukahara Toshifumi 北陸先端科学技術大学院大学, 先端科学技術研究科, 教授 (60207339)
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| Co-Investigator(Kenkyū-buntansha) |
鈴木 仁 北陸先端科学技術大学院大学, マテリアルサイエンス研究科, 助教 (00447690)
藤本 健造 北陸先端科学技術大学院大学, マテリアルサイエンス研究科, 教授 (90293894)
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| Co-Investigator(Renkei-kenkyūsha) |
Takada Shin'ichi 国立研究開発法人国立精神・神経医療研究センター, 神経研究所, 所長 (90171644)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
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| Keywords | 疾患治療 / 点変異 / RNA editing / 脱アミノ化 / 蛍光タンパク質 / 遺伝コード修復 / RFLP / 恒常的発現細胞 / 恒常的発言細胞株 / 恒常的発現細胞株 / RNA Editing / 恒常発現細胞株 |
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| Outline of Final Research Achievements |
Photochemical site-specific deamination was performed toward BFP-mRNA, by using oligodeoxynucleotides,ODNs, for inducing deamination. The base substitution by artificial RNA editing was confirmed by RT-PCR-RFLP analysis and fluorescence of in-vitro-translated proteins. Moreover, the mRNA sample was sequenced after photochemical RNA editing treatments, and confirmed that there was no mutation occurred except the target-site. We also investigated the ODN design that could induce efficient deamination. The ODN with complementary sequence length of 14 bases and hairpin length of 9 bases showed the highest photochemical RNA editing efficiency at 12.4%. This result suggests that the photochemical RNA editing is applicable as a theraputic method for genetic diseases.
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