High sensitive detection of single nucleotide mutation of virus genome by chemically modified-peptide nucleic acid

• Project/Area Number: 25290073
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Medical genome science
• Research Institution: Osaka University
• Principal Investigator: Kunihiro Kaihatsu 大阪大学, 産業科学研究所, 特任准教授 (70419464)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2015: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2013: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
• Keywords: 遺伝子診断 / 核酸塩基 / ペプチド核酸 / 塩基配列識別

Outline of Final Research Achievements

Peptide nucleic acid(PNA) is a DNA anaologue which has been known to possesses good sequence specificity. We introduced various types of tolane analogues at the end of PNA and succeeded to improve the sequence specificity. We studied if PNA-tolane can discriminate singel base-mismatched sequence that induces to the drug-resistance against an influenza virus drug. As a result, we found that PNA-tolane could detect the virus genome mutaion in a sequence specific manner.

Research Products

• [Journal Article] Rapid idenfitication of swine-origin influenza A virus by peptide nucleic acid-chromatography (2013)
  • Author(s): K. Kaihatsu, S. Sawada, N. Kato
  • Journal Title: Journal of Antivirals & Antiretrovirals Volume: 4 Issue: 04 Pages: 77-79
  • DOI: 10.4172/jaa.1000067
  • Peer Reviewed
• [Journal Article] Sequence-specific and visual identification of influenza virus gene by azobenzene-tethered bis-peptide nucleic acid (2013)
  • Author(s): K. Kaihatsu, S. Sawada, S. Nakamura, T. Nakaya, T. Yasunaga, N. Kato
  • Journal Title: PLoS ONE Volume: 8 Issue: 5 Pages: e64017-e64017
  • DOI: 10.1371/journal.pone.0064017
  • Peer Reviewed
• [Presentation] Sequence specific detection of RNA viral gene by chemically-modified peptide nucleic acid (2016)
  • Author(s): K. Kaihatsu, N. Kato
  • Organizer: The 6th Euro Virology Congress and Expo
  • Place of Presentation: Madrid, Spain
  • Year and Date: 2016-03-10
• [Presentation] Rapid identification of RNA viruses by peptide nucleic acid chromatography (2015)
  • Author(s): K. Kaihatsu, S. Sawada, K. Takagi, T. Hayashi, M.Okazaki, N. Kato
  • Organizer: Pacifichem-2015
  • Place of Presentation: Hawaii,USA
  • Year and Date: 2015-12-14
• [Presentation] Single-base-pair mismatch discrimination in DNA by tolane-modified peptide nucleic acid (2015)
  • Author(s): K. Kaihatsu,* K. Takagi, T. Hayashi, M. Okazaki, S. Sawada, N. Kato
  • Organizer: The 12th Bio-optics symposium
  • Place of Presentation: Shizuoka,Japan
  • Year and Date: 2015-11-27
• [Presentation] Improved DNA binding specificity of tolane-modified peptide nucleic acid and its application for virus detection. (2015)
  • Author(s): K. Kaihatsu,* K. Takagi, T. Hayashi, M. Okazaki, S. Sawada, N. Kato
  • Organizer: The 42nd International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: Himeji, Japan
  • Year and Date: 2015-09-23
• [Presentation] Improved Sequence-Specificity of Tolane-Modified Peptide Nucleic Acid (2014)
  • Author(s): Kunihiro Kaihatsu, Nobuo Kato
  • Organizer: 18th Sanken International Symposium 2014
  • Place of Presentation: 大阪市
  • Year and Date: 2014-12-10 – 2014-12-11
• [Presentation] Improved Sequence-Specificity of Tolane-Modified Peptide Nucleic Acid (2014)
  • Author(s): Kunihiro Kaihatsu
  • Organizer: 第10回バイオオプティクス研究会
  • Place of Presentation: 大阪府豊中市
  • Year and Date: 2014-12-05 – 2014-12-06
  • Invited
• [Presentation] Association behavior of tolane-modified peptide nucleic acid with ssDNA (2014)
  • Author(s): Kunihiro Kaihatsu, Nobuo Kato
  • Organizer: the 41st International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 北九州市
  • Year and Date: 2014-11-05 – 2014-11-07
• [Presentation] Effect of terminal functional group of peptide nucleic acid on its sequence specificity (2014)
  • Author(s): Kunihiro Kaihatsu, Kenji Takagi, Shinjiro Sawada, Tenko Hayashi, Miku Okazaki, Nobuo Kato
  • Organizer: the 41st International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 北九州市
  • Year and Date: 2014-11-05 – 2014-11-07
• [Presentation] Rapid identification of influenza A virus gene by peptide nucleic acid-chromatography (2014)
  • Author(s): Kunihiro Kaihatsu, Nobuo Kato
  • Organizer: 2nd Annual Microbiology & Infections Disease Asia Congress
  • Place of Presentation: Singapore
  • Year and Date: 2014-06-09 – 2014-06-12
  • Invited
• [Presentation] Effect of Non-natural Amino Acids on the Functions of Peptide and Peptide Nucleic Acid (2014)
  • Author(s): Kunihiro Kaihatsu, Nobuo Kato
  • Organizer: BIT's 7th Annual World Protein & Peptide Conference.
  • Place of Presentation: Dalian, China
  • Year and Date: 2014-04-24 – 2014-04-29
  • Invited
• [Presentation] Identification of Influenza Virus by Anti-Viral Gene Peptide Nucleic Acid (2014)
  • Author(s): Kunihiro Kaihatsu, Nobuo Kato
  • Organizer: The 17th Sanken International Symposium, The 2nd International Symposium of Nano-Macro Materials, Device, and System Research Alliance Project
  • Place of Presentation: 大阪大学
  • Invited
• [Presentation] Recognition of Single Base Mismatch within Single Strand DNA by Intercalator-Modified Peptide Nucleic Acid (2014)
  • Author(s): Miku Okazaki, Tenko Hayashi, Shinjiro Sawada, Kunihiro Kaihatsu, Nobuo Kato
  • Organizer: The 17th Sanken International Symposium, The 2nd International Symposium of Nano-Macro Materials, Device, and System Research Alliance Project
  • Place of Presentation: 大阪大学
• [Presentation] Effect of 5, 6-substituted pyrimidine bases on peptide nucleic acid duplex formation stability (2014)
  • Author(s): Kenji Takagi, Yiting Zhou, Kunihiro Kaihatsu, Nobuo Kato
  • Organizer: The 17th Sanken International Symposium, The 2nd International Symposium of Nano-Macro Materials, Device, and System Research Alliance Project
  • Place of Presentation: 大阪大学
• [Presentation] インターカレーター修飾ペプチド核酸によるDNA 一塩基識別 (2014)
  • Author(s): 岡崎実来、早矢仕恬子、澤田慎二郎、開發邦宏、加藤修雄
  • Organizer: 日本化学会第94回春季年会
  • Place of Presentation: 名古屋大学
• [Presentation] 非天然ピリミジン塩基を有するペプチド核酸の相補鎖会合特性 (2014)
  • Author(s): 高木賢至、周怡亭、開發邦宏、加藤修雄
  • Organizer: 日本化学会第94回春季年会
  • Place of Presentation: 名古屋大学
• [Presentation] Sequence-specific detection of a single base pair mismatches by tolane-modified peptide nucleic acid (2013)
  • Author(s): Miku Okazaki, Tenko Hayashi, Shinjiro Sawada, Kunihiro Kaihatsu, Nobuo Kato
  • Organizer: The 40th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: Kanagawa University
• [Presentation] Effect of pseudo-pyrimidine bases on peptide nucleic acid duplex formation stability (2013)
  • Author(s): Kenji Takagi, Yiting Zhou, Kunihiro Kaihatsu, Nobuo Kato
  • Organizer: The 40th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: Kanagawa University
• [Presentation] Diagnosis of influenza A/H1N1pdm virus genome by hairpin-type peptide nucleic acid chromatography (2013)
  • Author(s): Kunihiro Kaihatsu
  • Organizer: Option for the control of Influenza VIII
  • Place of Presentation: Capetown, South Africa
  • Invited
• [Presentation] ゲノム一塩基変異を識別する非天然核酸分子の創製 (2013)
  • Author(s): 開發邦宏
  • Organizer: 第１0回バイオオプティクス研究会
  • Place of Presentation: 東京農工大
  • Invited
• [Book] ペプチド核酸を利用したインフルエンザウイルスのゲノム診断法 (2013)
  • Author(s): 開發邦宏、加藤修雄
  • Total Pages: 5
  • Publisher: 化学工業
• [Remarks] ウイルス遺伝子の目視診断技術
  • URL: http://www.sanken.osaka-u.ac.jp/
• [Patent(Industrial Property Rights)] N末端にアルコキシ型トラン誘導体を有するペプチド核酸を用いた塩基配列識別技術 (2015)
  • Inventor(s): 開發邦宏、高木賢治、加藤修雄
  • Industrial Property Rights Holder: 開發邦宏、高木賢治、加藤修雄
  • Industrial Property Rights Type: 特許
  • Filing Date: 2015-09-17