High sensitive detection of single nucleotide mutation of virus genome by chemically modified-peptide nucleic acid
| Project/Area Number |
25290073
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical genome science
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2015: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
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| Keywords | 遺伝子診断 / 核酸塩基 / ペプチド核酸 / 塩基配列識別 |
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| Outline of Final Research Achievements |
Peptide nucleic acid(PNA) is a DNA anaologue which has been known to possesses good sequence specificity. We introduced various types of tolane analogues at the end of PNA and succeeded to improve the sequence specificity. We studied if PNA-tolane can discriminate singel base-mismatched sequence that induces to the drug-resistance against an influenza virus drug. As a result, we found that PNA-tolane could detect the virus genome mutaion in a sequence specific manner.
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Report
(4 results)
Research Products
(24 results)
