| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Outline of Final Research Achievements |
We were successful to make a NC-tagged protein array consisting of about 1,000 kinds of signal transduction proteins from 300 protein kinases and 700 singe transmembrane proteins, which these proteins were fused to N-terminally FLAG tag and C-terminally biotin ligation site to detect protein cleavage by AlphaScreen technology. We screened and found a protein cleaved by capase-1 (CASP1) in the protein array. A single cleavage site in a protein was identified by alanine mutagenesis. The new protein was also cleaved in the cells that CASP1 was activated and the C-terminally cleaved fragment was located in the nucleus although the N-terminal fragment seemed to be degradated. These results suggest that this protein is a novel substrate protein for CASP1.
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