Value-added advance of pathogenic fungus resources by comprehensive analysis of DNA as the indicator of species diversity

• Project/Area Number: 25290079
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Conservation of biological resources
• Research Institution: Chiba University
• Principal Investigator: Yaguchi Takashi 千葉大学, 真菌医学研究センター, 准教授 (60361440)
• Co-Investigator(Kenkyū-buntansha): 廣瀬 大 日本大学, 薬学部, 准教授 (20513922) ; 田中 玲子 千葉大学, 真菌医学研究センター, 助教 (60143319)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000); Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2015: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
• Keywords: 病原真菌 / 分類 / 薬剤感受性 / 次世代シーケンサー / MALDI-TOF-MS / 種多様性 / DNA / マイクロサテライト / 菌類 / 分類学 / 生態学

Outline of Final Research Achievements

We analyzed the whole-genome of Aspergillus fumigatus, A. niger and their related species preserved at the Medical mycology Center, Chiba Univ., using a next-generation DNA sequencer. Among the data, we examined the relationship between antifungal-resistance gene and MICs against antifungal. We also examined the MALDI-TOF-MS for ribosome protein on A. fumigatus and their related species and were able to detect each species. The data indicated the high validity of classification on these species. The new data were added to the data-base of pathogenic fungi and opened.

Research Products

• [Journal Article] Transcription factor Afmac1 controls copper import machinery in Aspergillus fumigatus has been accepted for publication. (2017)
  • Author(s): Kusuya Y, Hagiwara D, Sakai K, Yaguchi T, Gonoi T, Takahashi H.
  • Journal Title: Current Genetics Volume: 67 Issue: 4 Pages: 1-13
  • DOI: 10.1007/s00294-017-0681-z
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Capsule gene CAP64 is involved in the regulation of vacuole acidification in Cryptococcus neoformans. (2017)
  • Author(s): Imanishia Y, Tanaka R, Yaguchi T, Shimizu K.
  • Journal Title: Mycoscience Volume: 58 Issue: 1 Pages: 45-52
  • DOI: 10.1016/j.myc.2016.09.001
  • NAID: 40021065790
  • Peer Reviewed / Open Access
• [Journal Article] Asnovolins A-G, spiromeroterpenoids isolated from the fungus Aspergillus novofumigatus, and suppression of fibronectin expression by asnovolin E. (2016)
  • Author(s): Ishikawa K, Sato F, Itabashi T, Wachi H, Takeda H, Wakana D, Yaguchi T, Kawai K, Hosoe T.
  • Journal Title: J Nat Prod. Volume: 79 Issue: 9 Pages: 2167-2174
  • DOI: 10.1021/acs.jnatprod.6b00013
  • Peer Reviewed / Open Access
• [Journal Article] White-opaque switching in different mating type-like locus gene types of clinical Candida albicans isolates (2016)
  • Author(s): Li HM, Shimizu-Imanishi Y, Tanaka R, Li RY, Yaguchi T.
  • Journal Title: Chin Med J Volume: 129 Pages: 2725-2732
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Verification of Ribosomal Proteins of <i>Aspergillus fumigatus</i> for Use as Biomarkers in MALDI-TOF MS Identification (2016)
  • Author(s): Nakamura S, Sato H, Tanaka R, Yaguchi T.
  • Journal Title: Mass Spectrometry Volume: 5 Issue: 1 Pages: A0049-A0049
  • DOI: 10.5702/massspectrometry.A0049
  • NAID: 130005279669
  • ISSN: 2186-5116, 2187-137X
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Draft genome sequence of the pathogenic filamentous fungus Aspergillus lentulus IFM 54703T. (2016)
  • Author(s): Kusuya Y, Sakai K, Takahashi H, Yaguchi T
  • Journal Title: Genome Announc. Volume: 4 Issue: 1
  • DOI: 10.1128/genomea.01568-15
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Secondary metabolite profiles and antifungal drug susceptibility of Aspergillus fumigatus and closely related species, A. lentulus, A. udagawae, and A. viridinutans. (2015)
  • Author(s): Tamiya H, Ochiai E, Kikuchi K, Yahiro M, Toyotome T, Watanabe A, Yaguchi T, Kamei K.
  • Journal Title: J Infect Chemother Volume: 21 Issue: 5 Pages: 385-91
  • DOI: 10.1016/j.jiac.2015.01.005
  • Peer Reviewed / Open Access
• [Journal Article] Draft genome sequence of the pathogenic filamentous fungus Aspergillus udagawae strain IFM 46973T (2015)
  • Author(s): Kusuya Y, Takahashi-Nakaguchi A, Takahashi H, Yaguchi T
  • Journal Title: Genome Announc. Volume: 3(4) Issue: 4
  • DOI: 10.1128/genomea.00834-15
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] New mechanisms of flucytosine resistance in C. glabrata unveiled by a chemogenomics analysis in S. cerevisiae. (2015)
  • Author(s): Costa C, Ponte A, Pais P, Santos R, Cavalheiro M, Yaguchi T, Chibana H, Teixeira MC
  • Journal Title: PLoS One Volume: 10 Issue: 8 Pages: e0135110-e0135110
  • DOI: 10.1371/journal.pone.0135110
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] New species in Aspergillus section Fumigati from reclamation sites in Wyoming (U.S.A.) and revision of A. viridinutans complex (2014)
  • Author(s): Novakova A, Hubka V, Dudova Z, Matsuzawa T, Kubatova A, Yaguchi T, Kolarik M.
  • Journal Title: Fungal Diversity Volume: 64 Issue: 1 Pages: 253-274
  • DOI: 10.1007/s13225-013-0262-5
  • Peer Reviewed
• [Journal Article] Two new species of Aspergillus section Fumigati isolated from caatinga soil in the State of Pernambuco, Brazil. (2014)
  • Author(s): Matsuzawa T, Campos Takaki GM, Yaguchi T, Okada K, Gonoi T, Horie Y
  • Journal Title: Mycoscience Volume: 55 Issue: 2 Pages: 79-88
  • DOI: 10.1016/j.myc.2013.04.001
  • Peer Reviewed
• [Journal Article] Aspergillus huiyaniae sp. nov., a teleomorphic species in sect. Fumigati isolated from desert soil in China. (2014)
  • Author(s): Matsuzawa T, Horie Y, Abliz P, Gonoi T, Yaguchi T
  • Journal Title: Mycoscience Volume: 55 Issue: 3 Pages: 213-220
  • DOI: 10.1016/j.myc.2013.08.007
  • Peer Reviewed
• [Journal Article] Antifungal susceptibility of Aspergillus fumigatus clinical isolates collected from various areas in Japan (2014)
  • Author(s): Kikuchi K, Watanabe A, Ito J, Oku Y, Wuren T, Taguchi H, Yarita K, Muraosa Y, Yahiro M, Yaguchi T, Kamei K
  • Journal Title: J Infect Chemother Volume: 20 Issue: 5 Pages: 336-338
  • DOI: 10.1016/j.jiac.2014.01.003
  • Peer Reviewed
• [Journal Article] Aspergillus waksmanii sp. nov. and Aspergillus marvanovae sp. nov., two closely related species in section Fumigati described using polyphasic approach. (2013)
  • Author(s): sp. nov. and 84. Hubka V, Peterson SW, Frisvad JC, Yaguchi T, Kubátová A, Kolarík M.
  • Journal Title: Int J Syst Evol Microbiol Volume: 63 Issue: Pt_2 Pages: 783-789
  • DOI: 10.1099/ijs.0.047076-0
  • Peer Reviewed
• [Presentation] Isolation of Aspergillus fumigatus related species from the environment in Japan and their antifungal susceptibilities (2015)
  • Author(s): Takashi Yaguchi, Reiko Tanaka, Junko Ito, Dai Hirose, Akira Watanabe, Katsuhiko Kamei
  • Organizer: The 19th Congress of the International Society for Human and Animal Mycology
  • Place of Presentation: The Melbourne Convention Exhibition Centre, Melbourne, Australia
  • Year and Date: 2015-05-04
  • Int'l Joint Research
• [Presentation] Multilocus phylogeny, antifungal susceptibilities and mating behavior of opportunistic pathogen Aspergillus viridinutans (2014)
  • Author(s): Dudova Z, Hubka V, Svobodova L, Hamal P, Novakova A, Matsuzawa T, Yaguchi T, Kolarik M
  • Organizer: The 10th International Mycological Congress 2014
  • Place of Presentation: Bangkok,Thailand
  • Year and Date: 2014-08-03 – 2014-08-08
• [Presentation] 本邦環境中からの Aspergillus fumigatus及び関連の分離とその薬剤感受性 (2014)
  • Author(s): 矢口貴志, 伊藤純子, 今西由己, 廣瀬 大
  • Organizer: 日本菌学会
  • Place of Presentation: サイエンスヒルズこまつ（石川県小松市）
  • Year and Date: 2014-06-14 – 2014-06-15
• [Presentation] Antifungal susceptibility and frequency of Aspergillus fumigatus-related species of clinical strains collected as "A. fumigatus" from various area in Japan (2014)
  • Author(s): Watanabe A, Kikuchi K, Oku Y, Yarita K, Taguchi H, Yaguchi T, Kaamei K
  • Organizer: 6th Advances Against Aspergillosis
  • Place of Presentation: Madrid, Spain
• [Presentation] 菌種同定と薬剤感受性の問題点 (2013)
  • Author(s): 矢口貴志
  • Organizer: 第57回日本医真菌学会総会
  • Place of Presentation: 東京
  • Invited
• [Presentation] 本邦環境中からのAspergillus fumigatus 関連種の分離とその薬剤感受性 (2013)
  • Author(s): 矢口貴志、廣瀬 大、伊藤純子、松澤哲宏、村山琮明
  • Organizer: 第57回日本医真菌学会総会
  • Place of Presentation: 東京
• [Presentation] 本邦環境中からのAspergillus fumigatus 関連種の分離とその薬剤感受性
  • Author(s): 廣瀬 大、矢口貴志、伊藤純子、松澤哲宏、村山琮明、小川吉夫
  • Organizer: 第7回アスペルギルス研究会
  • Place of Presentation: 東京