Dynamic regulation of chromatin by histone chaperone
| Project/Area Number |
25291001
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OKUWAKI Mitsuru 筑波大学, グローバル教育院, 准教授 (50322699)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Keywords | 遺伝子 / ウイルス / ゲノム / 発生・分化 / クロマチン |
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| Outline of Final Research Achievements |
Aim of this study is to reveal the physiological function of histone chaperone TAF-I. TAF-I KO mice showed the embryonic lethality along with severe anemia and immature vascular remodeling until 12.5 day post conception. TAF-I KO ES cells showed the significant growth retardation and the delay of embryonic body formation mimicking the early embryo. From cell culture analyses, we found that TAF-I is involved in transcriptional regulation of interferon-stimulated genes through its histone H1 chaperone activity. From biochemical analyses, we revealed the regulatory mechanism of histone H1 chaperone activity of TAF-I through its intra-molecular binding. We also established the live cell imaging method of adenovirus genome in infected cells using TAF-I as a probe.
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Report
(4 results)
Research Products
(50 results)
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[Journal Article] Long Noncoding RNA NEAT1-Dependent SFPQ Relocation from Promoter Region to Paraspeckle Mediates IL8 Expression upon Immune Stimuli2014
Author(s)
Imamura K, Imamachi N, Akizuki G, Kumakura M, Kawaguchi A, Nagata K, Kato A, Kawaguchi Y, Sato H, Yoneda M, Kai C, Yada T, Suzuki Y, Yamada T, Ozawa T, Kaneki K, Inoue T, Kobayashi M, Kodama T, Wada Y, Sekimizu K and Akimitsu N.
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Journal Title
Molecular cell
Volume: 53
Issue: 3
Pages: 393-406
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Antagonistic activities of the immunomodulator and PP2A-activating drug FTY720 (Fingolimod, Gilenya) in Jak2-driven hematologic malignancies.2013
Author(s)
Oaks JJ, Santhanam R, Walker CJ, Roof S, Harb JG, Ferenchak G, Eisfeld AK, Van Brocklyn JR, Briesewitz R, Saddoughi SA, Nagata K, Bittman R, Caligiuri MA, Abdel-Wahab O, Levine R, Arlinghaus RB, Quintas-Cardama A, Goldman JM, Apperley J, Reid A, Milojkovic D, Ziolo MT, Marcucci G, Ogretmen B, Neviani P, Perrotti D.
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Journal Title
Blood
Volume: 122(11)
Issue: 11
Pages: 1923-1934
DOI
Related Report
Peer Reviewed
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