| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
|
|---|
| Outline of Final Research Achievements |
Aim of this study is to reveal the physiological function of histone chaperone TAF-I. TAF-I KO mice showed the embryonic lethality along with severe anemia and immature vascular remodeling until 12.5 day post conception. TAF-I KO ES cells showed the significant growth retardation and the delay of embryonic body formation mimicking the early embryo. From cell culture analyses, we found that TAF-I is involved in transcriptional regulation of interferon-stimulated genes through its histone H1 chaperone activity. From biochemical analyses, we revealed the regulatory mechanism of histone H1 chaperone activity of TAF-I through its intra-molecular binding. We also established the live cell imaging method of adenovirus genome in infected cells using TAF-I as a probe.
|
