Molecular pathogenesis of ribosomopathies and defective ribosome CODE

• Project/Area Number: 25291003
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Molecular biology
• Research Institution: University of Miyazaki
• Principal Investigator: Kenmochi Naoya 宮崎大学, フロンティア科学実験総合センター, 教授 (00133124)
• Co-Investigator(Renkei-kenkyūsha): Suzuki Tsutomu 東京大学, 工学研究科, 教授 (20292782) ; Suzuki Yutaka 東京大学, 新領域創成科学研究科, 教授 (40323646)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2015: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
• Keywords: リボソーム / 疾患モデル / ゼブラフィッシュ / 造血異常 / 核小体 / 発がん / 飜訳 / ダイアモンド・ブラックファン貧血 / ダイヤモンド・ブラックファン貧血 / 翻訳

Outline of Final Research Achievements

Ribosomopathies are the diseases caused by ribosomal abnormalities. We hypothesized that the selected mRNA translation by ribosomes should play an important role in the disease onset. To investigate the molecular pathogenesis of ribosomopathies, we developed a zebrafish model of Diamond-Blackfun anemia by knocking down a ribosomal protein gene RPS19 and found that the genes involved in erythropoiesis and glycan biosynthesis were translationally down-regulated. We also investigated the role of rRNA modifications in zebrafish and found that the modifications are essential for the early development of zebrafish and a loss of the modification leads to translational dysregulation of mRNAs. These results indicate that regulation of the selected mRNA translation is associated with the pathogenic mechanism of ribosomopathies.

Research Products

• [Int'l Joint Research] University of Missouri-Kansas City(米国)
• [Journal Article] Exome sequencing identified RPS15A as a novel causative gene for Diamond-Blackfan anemia. (2017)
  • Author(s): Ikeda F, Yoshida K, Toki T, Uechi T, Ishida S, Nakajima Y, Sasahara Y, Okuno Y, Kanezaki R, Terui K, Kamio T, Kobayashi A, Fujita T, Sato-Otsubo A, Shiraishi Y, Tanaka H, Chiba K, Muramatsu H, Kanno H, Ohga S, Ohara A, Kojima S, Kenmochi N, Miyano S, Ogawa S, Ito E.
  • Journal Title: Haematologica. Volume: - Issue: 3 Pages: e93-e96
  • DOI: 10.3324/haematol.2016.153932
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] TBCD may be a causal gene in progressive neurodegenerative encephalopathy with atypical infantile spinal muscular atrophy (2017)
  • Author(s): Ikeda T, Nakahara A, Nagano R, Utoyama M, Obara M, Moritake H, Uechi T, Mitsui J, Ishiura H, Yoshimura J, Doi K, Kenmochi N, Morishita S, Nishino I, Tsuji S, and Nunoi H
  • Journal Title: J Hum Genet Volume: 62 Issue: 4 Pages: 473-480
  • DOI: 10.1038/jhg.2016.149
  • NAID: 40021158250
  • Peer Reviewed / Open Access
• [Journal Article] RNAcentral: a comprehensive database of non-coding RNA sequences (2017)
  • Author(s): The RNAcentral Consortium
  • Journal Title: Nucleic Acids Res Volume: 45 Issue: D1 Pages: D128-D134
  • DOI: 10.1093/nar/gkw1008
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Incomplete splicing of neutrophil-specific genes affects neutrophil development in a zebrafish model of poikiloderma with neutropenia (2015)
  • Author(s): Patil P, Uechi T, Kenmochi N
  • Journal Title: RNA Biology Volume: 12 Issue: 4 Pages: 426-434
  • DOI: 10.1080/15476286.2015.1017240
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] scaRNAs regulate splicing and vertebrate heart development. (2015)
  • Author(s): Patil, P., Kibiryeva, N., Uechi, T., Marshall, J., Artman, M., O'Brien, J.E., Kenmochi, N. and Bittel, D.C.
  • Journal Title: Biochimica et Biophysica Acta-Molecular Basis of Disease Volume: 未定 Issue: 8 Pages: 1619-1629
  • DOI: 10.1016/j.bbadis.2015.04.016
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] scaRNAs regulate splicing and vertebrate heart development (2015)
  • Author(s): Patil P, Kibiryeva N, Uechi T, Marshall J, Artman M, O'Brien JE, Kenmochi N, Bittel, DC
  • Journal Title: Biochem. Biophys. Acta (in press) Volume: 0 Pages: 0-0
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Loss of function mutations in RPL27 and RPS27 identified by whole-exome sequencing in Diamond-Blackfan anaemia. (2015)
  • Author(s): Wang R, Yoshida K, Toki T, Sawada T, Uechi T, Okuno Y, Sato-Otsubo A, Kudo K, Kamimaki I, Kanezaki R, Shiraishi Y, Chiba K, Tanaka H, Terui K, Sato T, Iribe Y, Ohga S, Kuramitsu M, Hamaguchi I, Ohara A, Hara J, et al.
  • Journal Title: British Journal of Haematology Volume: 168 Issue: 6 Pages: 854-864
  • DOI: 10.1111/bjh.13229
  • Peer Reviewed / Open Access
• [Journal Article] Ribosomal protein deficiency causes Tp53-independent erythropoiesis failure in zebrafish (2014)
  • Author(s): Yadav V.G., Chakraborty A., Uechi T., and Kenmochi N.
  • Journal Title: Int. J. Biochem. Cell Biol. Volume: 49 Pages: 1-7
  • DOI: 10.1016/j.biocel.2014.01.006
  • Peer Reviewed
• [Journal Article] リボソーム病ーリボソーム合成の異常と疾患ー (2013)
  • Author(s): 剣持直哉
  • Journal Title: 生化学 Volume: 85(10) Pages: 909-915
• [Journal Article] snOPY : a small nucleolar RNA orthological gene database (2013)
  • Author(s): Yoshihama M., Nakao A., and Kenmochi N.
  • Journal Title: BMC Research Notes Volume: 6 Issue: 1 Pages: 426-426
  • DOI: 10.1186/1756-0500-6-426
  • Peer Reviewed
• [Presentation] Decreased translational efficiency of mRNAs required for hematopoiesis in a zebrafish model of Diamond-Blackfan anemia (2017)
  • Author(s): Tamayo Uechi, Maki Yoshihama, Yukari Nakajima, Yutaka Suzuki, Sumio Sugano, Naoya Kenmochi
  • Organizer: "Translation Machinery in Health & Disease" Gordon Research Conference
  • Place of Presentation: Galveston, USA
  • Year and Date: 2017-03-19
  • Int'l Joint Research
• [Presentation] Zebrafish: an in vivo model for human congenital disorders (2016)
  • Author(s): Prakash Patil, K.J. Palaksha, Tamayo Uechi, Naoya Kenmochi
  • Organizer: 9th Karnataka Science and Technology Academy (KSTA) Conference
  • Place of Presentation: Bangalore, India
  • Year and Date: 2016-12-20
  • Int'l Joint Research
• [Presentation] リボソームによる翻訳制御と疾患：ゼブラフィッシュを用いた解析 (2016)
  • Author(s): 上地 珠代, 中島 由香里, 吉浜 麻生, 鈴木 穣, 菅野 純夫, 剣持 直哉
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2016-11-30
• [Presentation] ゼブラフィッシュDBAモデルを用いた創薬のための in vivo スクリーニング (2016)
  • Author(s): 吉浜 麻生, 上地 珠代, 中島 由香里, 引間 園美, 剣持 直哉
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2016-11-30
• [Presentation] ゼブラフィッシュを用いたダイアモンド・ブラックファン貧血の発症機構の解析 (2016)
  • Author(s): 上地 珠代, 中島 由香里, 吉浜 麻生, 鈴木 穣, 菅野 純夫, 剣持 直哉
  • Organizer: 第89回日本生化学会大会
  • Place of Presentation: 仙台
  • Year and Date: 2016-09-25
• [Presentation] ゼブラフィッシュをモデルとしたPAPS合成酵素の整理機能解明 (2016)
  • Author(s): 上中 勝護, 上地 珠代, 剣持 直哉, 黒木 勝久, Liu Ming-Cheh, 水光 正仁, 榊原 陽一
  • Organizer: 第89回日本生化学会大会
  • Place of Presentation: 仙台
  • Year and Date: 2016-09-25
• [Presentation] ゼブラフィッシュを用いたダイアモンド・ブラックファン貧血発症機構の解析および創薬スクリーニング (2016)
  • Author(s): 上地 珠代, 吉浜 麻生, 中島 由香里, 鈴木 穣, 伊藤 悦朗, 剣持 直哉
  • Organizer: 第4回RIBOSOME MEETING
  • Place of Presentation: 大阪
  • Year and Date: 2016-09-17
• [Presentation] ダイアモンド・ブラックファン貧血で同定された新たな原因候補遺伝子RPS15Aに関するゼブラフィッシュモデルを用いた解析 (2016)
  • Author(s): 石田 詩織, 上地 珠代, 池田 史圭, 土岐 力, 吉田 健一, 小川 誠司, 伊藤 悦朗, 剣持 直哉
  • Organizer: 第4回RIBOSOME MEETING
  • Place of Presentation: 大阪
  • Year and Date: 2016-09-17
• [Presentation] Selected mRNA translation and ribosomopathies: analyzing the zebrafish model of congenital pure red-cell aplasia (2016)
  • Author(s): Tamayo Uechi, Yukari Nakajima, Maki Yoshihama, Yutaka Suzuki, Sumio Sugano, Naoya Kenmochi
  • Organizer: RNA2016
  • Place of Presentation: 京都
  • Year and Date: 2016-06-28
  • Int'l Joint Research
• [Presentation] RPG and snOPY: Databases for ribosomal protein genes and small nucleolar RNA genes (2016)
  • Author(s): Maki Yoshihama, Hiroki Tachiyama, Shusaku Kamada, Akihiro Nakao, Naoya Kenmochi
  • Organizer: RNA2016
  • Place of Presentation: 京都
  • Year and Date: 2016-06-28
  • Int'l Joint Research
• [Presentation] Role of rRNA modification in zebrafish development (2016)
  • Author(s): Naoya Kenmochi, Tamayo Uechi, Sayomi Higa-Nakamine, Maki Yoshihama, Tsutomu Suzuki
  • Organizer: EMBL Conference on The Epitranscriptome
  • Place of Presentation: Heidelberg, Germany
  • Year and Date: 2016-04-20
  • Int'l Joint Research
• [Presentation] Ribosomal dysfunction leads to erythropoiesis failure in a zebrafish model of Diamond-Blackfan anemia (2016)
  • Author(s): Naoya Kenmochi, Tamayo Uechi, Yukari Nakajima, Maki Yoshihama, Yutaka Suzuki, Sumio Sugano
  • Organizer: The 13th International Congress of Human Genetics
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2016-04-03
  • Int'l Joint Research
• [Presentation] Ribosomal dysfunction leads to erythropoiesis failure in a zebrafish model of Diamond-Blackfan anemia (2016)
  • Author(s): Naoya Kenmochi, Tamayo Uechi, Yukari Nakajima, Maki Yoshihama, Yutaka Suzuki, Sumio Sugano
  • Organizer: The 13th International Congress of Human Genetics
  • Place of Presentation: 京都
  • Year and Date: 2016-04-02
  • Int'l Joint Research
• [Presentation] Investigation of the cardiovascular toxicity of oxidized cholesterols using the zebrafis as an animal model (2016)
  • Author(s): Katsuhisa Kurogi, Maki Yoshihama, Naoya Kenmochi, Yoichi Sakakibara, Masahito Suiko, Frederick Williams, Ming-Cheh Liu
  • Organizer: American Society for Biochemistry and Molecular Biology
  • Place of Presentation: San Diego, USA
  • Year and Date: 2016-04-02
  • Int'l Joint Research
• [Presentation] Aberrant translation and ribosomopathies: studying the molecular pathogenesis of human diseases using zebrafish as a model animal (2016)
  • Author(s): Tamayo Uechi, Yukari Nakajima, Maki Yoshihama, Shiori Ishida, Tsutomu Toki, Yutaka Suzuki, Sumio Sugano, Etsuro Ito and Naoya Kenmochi
  • Organizer: The International Meeting on Aquatic Model Organism for Human Disease and Toxicology Research
  • Place of Presentation: Okazaki, Japan
  • Year and Date: 2016-03-18
  • Int'l Joint Research
• [Presentation] Decreased translation efficiency of mRNAs required for hematopoiesis in a zebrafish model of Diamond-Blackfan anemia (2016)
  • Author(s): Tamayo Uechi, Yukari Nakajima, Maki Yoshihama, Tsutomu Toki, Yutaka Suzuki, Sumio Sugano, Etsuro Ito and Naoya Kenmochi
  • Organizer: The 14th Diamond Blackfan Anemia International Consensus Conference
  • Place of Presentation: Atlanta, USA
  • Year and Date: 2016-03-05
  • Int'l Joint Research
• [Presentation] ノックダウン胚を用いた化合物スクリーニングおよび薬物代謝の in vivo 解析 (2016)
  • Author(s): 吉浜 麻生, 上地 珠代, 黒木 勝久, 鈴木 穣, Ming-Cheh Liu, 剣持 直哉
  • Organizer: 第2回ゼブラフィッシュ創薬研究会
  • Place of Presentation: 岐阜
• [Presentation] ゼブラフィッシュを用いたダイアモンド・ブラックファン貧血の新たな原因遺伝子の同定 (2015)
  • Author(s): 石田詩織, 上地珠代, 池田史圭, 土岐力, 吉田健一, 小川誠司, 伊藤悦朗, 剣持直哉
  • Organizer: 第38回日本分子生物学会年会・第88回日本生化学会大会合同大会
  • Place of Presentation: 神戸コンベンションセンター（神戸市中央区）
  • Year and Date: 2015-12-01
• [Presentation] ゼブラフィッシュを用いたリボソーム病発症メカニズムの解明 (2015)
  • Author(s): 上地珠代, 中島由香里, 吉浜麻生, 鈴木穣, 菅野純夫, 剣持直哉
  • Organizer: 第38回日本分子生物学会年会・第88回日本生化学会大会合同大会
  • Place of Presentation: 神戸コンベンションセンター（神戸市中央区）
  • Year and Date: 2015-12-01
• [Presentation] リボソーム病モデルを用いた発症機序の解明と化合物スクリーニング (2015)
  • Author(s): 上地珠代, 吉浜麻生, 中島由香里, 上米良美香, 剣持直哉
  • Organizer: 第1回ゼブラフィッシュ創薬研究会
  • Place of Presentation: 三重大学医学部（津市江戸橋）
  • Year and Date: 2015-11-06
• [Presentation] Exome sequencing identified RPS15A as a novel causative gene in a Dimond-Blackfan anemia family (2015)
  • Author(s): Fumika Ikeda, Kenichi Yoshida, Tsutomu Toki, Rika Kanezaki, Kiminori Terui, Yoji Sasahara, Aiko Sato-Otsubo, Yuichi Shiraishi, Kenichi Chiba, Hitoshi Kanno, Shouichi Ohga, Akira Ohara, Seiji Kojima, Tamayo Uechi, Naoya Kenmochi, Satoru Miyano, Seishi Ogawa, Etsuro Ito
  • Organizer: 第77回日本血液学会学術集会
  • Place of Presentation: 石川県立音楽堂（金沢市昭和町）
  • Year and Date: 2015-10-16
• [Presentation] Selected mRNA translation and ribosomopathies: studying the molecular pathogenesis of congenital anemia using zebrafish as a model system (2015)
  • Author(s): Tamayo Uechi, Yukari Nakajima, Maki Yoshihama, Yutaka Suzuki, Sumio Sugano, and Naoya Kenmochi
  • Organizer: International Conference on Ribosome Synthesis
  • Place of Presentation: Brussels, Belgium
  • Year and Date: 2015-08-19
  • Int'l Joint Research
• [Presentation] ゼブラフィッシュを用いたリボソーム病発症機構の解明 (2015)
  • Author(s): 上地珠代, 中島由香里, グナネッシュワ ヤダフ, 吉浜麻生, 鈴木穣, 菅野純夫, 剣持直哉
  • Organizer: 第3回リボソームミーティング
  • Place of Presentation: 宮崎
  • Year and Date: 2015-03-17 – 2015-03-18
• [Presentation] Ribosomal Dysfunction and Defective Erythropoiesis in a Zebrafish Model of Diamond-Blackfan Anemia (2015)
  • Author(s): Tamayo Uechi, Yukari Nakajima, Gnaneshwar Yadav, Maki Yoshihama, Yutaka Suzuki, Sumio Sugano, and Naoya Kenmochi
  • Organizer: Translation Machinery in Health & Disease (Gordon Research Conferences)
  • Place of Presentation: Ventura, USA
  • Year and Date: 2015-02-22 – 2015-02-27
• [Presentation] ゼブラフィッシュ初期発生におけるRNA修飾の役割 (2014)
  • Author(s): 剣持直哉
  • Organizer: 第37回分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
  • Invited
• [Presentation] 選択的な翻訳制御と疾患：ゼブラフィッシュを用いたリボソーム病発症機構の解明 (2014)
  • Author(s): 上地珠代, 中島由香里, Gnaneshwar Yadav, 吉浜麻生, 鈴木穣, 菅野純夫, 剣持直哉
  • Organizer: 第37回分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Defects in ribosomal proteins cause erythroid failure in a zebrafish model of Diamond-Blackfan anemia (2014)
  • Author(s): Tamayo Uechi, Yukari Nakajima, Gnaneshwar Yadav, Maki Yoshihama, Yutaka Suzuki, Sumio Sugano, and Naoya Kenmochi
  • Organizer: 第20回小型魚類研究会
  • Place of Presentation: 東京
  • Year and Date: 2014-09-20 – 2014-09-21
• [Presentation] Studying the molecular pathogenesis of Diamond-Blackfan anemia using zebrafish as a model system (2014)
  • Author(s): Tamayo Uechi, Yukari Nakajima, Gnaneshwar Yadav, Maki Yoshihama, Yutaka Suzuki, Sumio Sugano, and Naoya Kenmochi
  • Organizer: The 14th Conference on Translational Control
  • Place of Presentation: Cold Spring Harbor, USA
  • Year and Date: 2014-09-02 – 2014-09-06
• [Presentation] RPG and snOPY: Databases for ribosomal protein genes and small nucleolar RNA genes (2014)
  • Author(s): Maki Yoshihama, Akihiro Nakao, Shusaku Kamada, Naoya Kenmochi
  • Organizer: The 19th Annual Meeting of the RNA Society
  • Place of Presentation: Quebec, Canada
  • Year and Date: 2014-06-03 – 2014-06-08
• [Presentation] Ribosome dysfunction and erythroid failure: Analyzing the zebrafish model of Diamond-Blackfan anemia (2014)
  • Author(s): Tamayo Uechi, Yukari Nakajima, Gnaneshwar Yadav, Yutaka Suzuki, Sumio Sugano, and Naoya Kenmochi
  • Organizer: The 19th Annual Meeting of the RNA Society
  • Place of Presentation: Quebec, Canada
  • Year and Date: 2014-06-03 – 2014-06-08
• [Presentation] Analyzing the zebrafish model of Diamond-Blackfan anemia (2014)
  • Author(s): Tamayo Uechi, Yukari Nakajima, Gnaneshwar V. Yadav, Yutaka Suzuki, Sumio Sugano, Naoya Kenmochi
  • Organizer: 3th Diamond Blackfan Anemia International Consensus Conference
  • Place of Presentation: Atlanta, USA
• [Presentation] A detection method for snoRNA modification domain by fully indexable dictionary retrieving (2014)
  • Author(s): Takayuki Yamamoto, Kunihito Yamamori, Naoya Kenmochi, Masaru Aikawa
  • Organizer: AROB 19th 2014
  • Place of Presentation: 別府
• [Presentation] リボソームRNAの修飾はゼブラフィッシュの初期発生に必須の役割を果たす (2013)
  • Author(s): 剣持 直哉
  • Organizer: 第36回 日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Invited
• [Presentation] RPG and snOPY: リボソームタンパク質遺伝子およびsnoRNA遺伝子データベースの構築 (2013)
  • Author(s): 吉浜 麻生、中尾 彰宏、上地 珠代、剣持 直哉
  • Organizer: 第36回 日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] ダイアモンド・ブラックファン貧血の新たな責任遺伝子の同定：ゼブラフィッシュモデルを用いた解析 (2013)
  • Author(s): 澤田 尚史、上地 珠代、土岐 力、王 汝南、吉田 健一、小川 誠司、伊藤 悦朗、剣持 直哉
  • Organizer: 第36回 日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] Zebrafish model of Diamond-Blackfan anemia: Ribosomal protein deficiency causes Tp53-independent erythropoiesis failure (2013)
  • Author(s): Gnaneshwar Yadav, Anirban Chakraborty, Tamayo Uechi, Naoya Kenmochi
  • Organizer: 第36回 日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] Erythropoiesis failure and ribosomal dysfunction in zebrafish model of Diamond-Blackfan anemia (2013)
  • Author(s): Tamayo Uechi, Yukari Nakajima, Gnaneshwar Yadav, Takafumi Sawada, Masahiro Ikeda, Naoya Kenmochi
  • Organizer: The 62nd Annual Meeting of the American Society of Human Genetics
  • Place of Presentation: Boston, USA
• [Presentation] ゼブラフィッシュを用いたリボソーム病発症機構の解明 (2013)
  • Author(s): 上地 珠代、中島 由香里、ヤダフ グナネシュワ、パティル プルカッシュ、鈴木 穣、菅野 純夫、剣持 直哉
  • Organizer: 第15回RNAミーティング
  • Place of Presentation: 愛媛県民文化会館（松山）
• [Presentation] Molecular pathogenesis of ribosomopaties in zebrafish model for Diamond-Blackfan anemia (2013)
  • Author(s): Tamayo Uechi, Yukari Nakajima, Hiroko Sonoda, Masahiro Ikeda, Yutaka Suzuki, Sumio Sugano, Naoya Kenmochi
  • Organizer: The 18th annual meeting of the RNA Society
  • Place of Presentation: Davos, Switzerland
• [Presentation] When p53 senses faulty ribosomes: Induction of Tp53 correlates with enhanced expression of c-Myc target nucleolar proteins in Rpl11-deficient zebrafish (2013)
  • Author(s): Anirban Chakraborty, Tamayo Uechi, Pierre-Emmanuel Gleizes, Naoya Kenmochi
  • Organizer: The 18th annual meeting of the RNA Society
  • Place of Presentation: Davos, Switzerland
• [Book] 大・中・小動物実験プロトコル. 第4章 動物実験手法：ゼブラフィッシュを用いた疾患モデルの作製・解析 (2016)
  • Author(s): 剣持直哉, 上地珠代
  • Total Pages: 146
  • Publisher: 宮日文化情報センター
• [Book] ノンコーディングRNA－RNA分子の全体像を俯瞰する. 第4章 snoRNA (2016)
  • Author(s): 剣持直哉, 吉浜麻生
  • Total Pages: 372
  • Publisher: 化学同人
• [Book] 非コードRNA：第4章snoRNA (2016)
  • Author(s): 剣持直哉，吉浜麻生
  • Publisher: 化学同人
• [Book] 動物実験プロトコール：ゼブラフィッシュを用いた疾患モデルの作製・解析 (2016)
  • Author(s): 剣持直哉，上地珠代
  • Total Pages: 146
  • Publisher: 宮日文化情報センター
• [Remarks] 研究室ホームページ
  • URL: http://ribosome-labo.med.miyazaki-u.ac.jp
• [Remarks] リボソームタンパク質遺伝子データベース（RPG）
  • URL: http://ribosome.med.miyazaki-u.ac.jp/
• [Remarks] snoRNA遺伝子データベース
  • URL: http://snoopy.med.miyazaki-u.ac.jp/
• [Remarks] 剣持研究室ホームページ
  • URL: http://ribosome.med.miyazaki-u.ac.jp/labo/
• [Remarks] リボソームタンパク質遺伝子データベース
  • URL: http://ribosome.med.miyazaki-u.ac.jp/
• [Remarks] ゼブラフィッシュRP遺伝子ノックダウンデータベース
  • URL: http://zebrafish.med.miyazaki-u.ac.jp/
• [Remarks] 剣持研究室ホームページ
  • URL: http://ribosome.med.miyazaki-u.ac.jp/labo/
• [Remarks] リボソームタンパク質遺伝子データベース
  • URL: http://ribosome.med.miyazaki-u.ac.jp/
• [Remarks] snoRNA遺伝子データベース
  • URL: http://snoopy.med.miyazaki-u.ac.jp/
• [Remarks] ゼブラフィッシュRP遺伝子ノックダウンデータベース
  • URL: http://zebrafish.med.miyazaki-u.ac.jp/