Studies of nascent chains that monitors protein localization machineries
| Project/Area Number |
25291006
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Kyoto Sangyo University |
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Principal Investigator |
CHIBA Shinobu 京都産業大学, 総合生命科学部, 准教授 (20523517)
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| Co-Investigator(Renkei-kenkyūsha) |
ITO Koreaki 京都産業大学, 研究機構, シニアリサーチフェロー (90027334)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2013: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Keywords | 翻訳アレスト / 翻訳途上鎖 / タンパク質局在化 / 翻訳制御 / タンパク質膜組込 / 蛋白質膜組込 |
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| Outline of Final Research Achievements |
To understand the molecular mechanisms of how regulatory arrest peptides, such as Bacillus subtilis MifM or Escherichia coli SecM could regulate their own translation elongation, we studied interactions between nascent polypeptides and the ribosomes. Our genetic, biochemical or structural studies revealed several detail features of interactions between nascent chain and the ribosome in the exit tunnel as well as near the active center of the ribosome. We also studied YidC, a membrane protein insertase that is required for releasing elongation arrest of MifM, and found several biochemical properties of the YidC that are important for its functions.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Hydrophilic microenvironment required for the channel-independent insertase function of YidC protein.2015
Author(s)
Shimokawa-Chiba, N., Kumazaki, K., Tsukazaki, T., Nureki, O., Ito, K. and Chiba, S.
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Journal Title
Proc. Natl. Acad. Sci. USA.
Volume: 112
Issue: 16
Pages: 5063-5068
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Structure of the Bacillus subtilis 70S ribosome reveals the basis for species-specific stalling.2015
Author(s)
Sohmen, D., Chiba, S., Shimokawa-Chiba, N., Innis, A., Berninghausen, O., Beckmann, R., Ito, K. and Wilson, D.
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Journal Title
Nat. Commun.
Volume: 6
Issue: 1
Pages: 6941-6941
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Nascent chain-monitored remodeling of the Sec machinery for salinity adaptation of marine bacteria2015
Author(s)
Ishii, E., Chiba, S., Hashimoto, N., Kojima, S., Homma, M., Ito, K., Akiyama, Y. and Mori, H.
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 112
Issue: 40
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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