Structural biology of multidrug transporters

• Project/Area Number: 25291011
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: The University of Tokyo
• Principal Investigator: Ishitani Ryuichiro 東京大学, 理学(系)研究科(研究院), 准教授 (90361568)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: X線結晶構造解析 / 膜蛋白質 / 多剤耐性菌 / 膜輸送体 / Ｘ線結晶構造解析 / 膜タンパク質 / 薬物動態

Outline of Final Research Achievements

Multidrug efflux transporters recognize and export various toxic compounds, thereby conferring antibiotics resistance to the multidrug-resistance pathogens. Understanding of this substrate recognition and export mechanism by multidrug exporters is important from the point of the view of drug discovery. However, the structural analysis of membrane transporters is very difficult. As a result of recent advance of the structural analysis, several crystal structures of multidrug efflux transporters have been determined, but their resolutions were not enough to understand the biologically important aspects, such as the substrate recognition mechanism. In this research project, we performed the structural and functional analyses of YddG, which was initially identified as an efflux pump of toxic compounds, and then shown to export aromatic amino acids by subsequent researches.

Research Products

• [Journal Article] Crystal structures of the TRIC trimeric intracellular cation channel orthologues. (2016)
  • Author(s): Kasuya G, Hiraizumi M, Maturana AD, Kumazaki K, Fujiwara Y, Liu K, Nakada-Nakura Y, Iwata S, Tsukada K, Komori T, Uemura S, Goto Y, Nakane T, Takemoto M, Kato HE, Yamashita K, Wada M, Ito K, Ishitani R, Hattori M, Nureki O.
  • Journal Title: Cell Res. Volume: 26 Issue: 12 Pages: 1288-1301
  • DOI: 10.1038/cr.2016.140
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structural and functional analysis of DDX41: a bispecific immune receptor for DNA and cyclic dinucleotide. (2016)
  • Author(s): Omura H, Oikawa D, Nakane T, Kato M, Ishii R, Ishitani R, Tokunaga F, and Nureki O.
  • Journal Title: Sci. Rep. Volume: 6 Issue: 1 Pages: 34756-34756
  • DOI: 10.1038/srep34756
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Crystal structure of silkworm PIWI-clade Argonaute Siwi bound to piRNA (2016)
  • Author(s): Matsumoto N, Nishimasu H, Sakakibara K, Nishida KM, Hirano T, Ishitani R, Siomi H, Siomi MC, Nureki O
  • Journal Title: Cell Volume: 167 Issue: 2 Pages: 484-497
  • DOI: 10.1016/j.cell.2016.09.002
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Breakpoint cluster region-mediated inflammation is dependent on casein kinase II. (2016)
  • Author(s): Meng, J., J-J Jiang, T. Atsumi, H. Bando, Y. Okuyama, L. Sabharwal, I. Nakagawa, H. Higuchi, M. Ota, M. Okawara, R. Ishitani, O. Nureki, D. Higo, Y. Arima, H. Ogura, D. Kamimura and M. Murakami.
  • Journal Title: J Immunol. Volume: 197(8) Issue: 8 Pages: 3111-3119
  • DOI: 10.4049/jimmunol.1601082
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Linear ubiquitination is involved in the pathogenesis of optineurin-associated amyotrophic lateral sclerosis. (2016)
  • Author(s): Nakazawa S, Oikawa D, Ishii R, Ayaki T, Takahashi H, Takeda H, Ishitani R, Kamei K, Takeyoshi I, Kawakami H, Iwai K, Hatada I, Sawasaki T, Ito H, Nureki O, and Tokunaga F.
  • Journal Title: Nat. Commun. Volume: 7 Issue: 1 Pages: 12547-12547
  • DOI: 10.1038/ncomms12547
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Crystal structure of the plant receptor-like kinase TDR in complex with the TDIF peptide. (2016)
  • Author(s): Morita, J., Kato, K., Nakane, T., Kondo, Y., Fukuda, H., Nishimasu, H., *Ishitani, R. and *Nureki, O.
  • Journal Title: Nat. Commun. Volume: 7 Issue: 1 Pages: 12383-12383
  • DOI: 10.1038/ncomms12383
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Structural and functional insights into IZUMO1 recognition by JUNO in mammalian fertilization. (2016)
  • Author(s): Kato K, Satouh Y, Nishimasu H, Kurabayashi A, Morita J, Fujihara Y, Oji A, Ishitani R, Ikawa M, Nureki O.
  • Journal Title: Nature Communications Volume: 7 Issue: 1 Pages: 12198-12198
  • DOI: 10.1038/ncomms12198
  • NAID: 120006957420
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] LCP crystallization and X-ray diffraction analysis of VcmN, a MATE transporter from Vibrio cholerae. (2016)
  • Author(s): 2.Kusakizako, T.; Tanaka, Y.; Hipolito, C. J.; Kuroda, T.; Ishitani, R.; Suga, H.; Nureki, O.
  • Journal Title: Acta Crystallogr. F Struct. Biol. Commun. Volume: 72(Pt 7) Issue: 7 Pages: 552-557
  • DOI: 10.1107/s2053230x16008931
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structural basis for amino-acid export by DMT superfamily transporter YddG (2016)
  • Author(s): H. Tsuchiya, S. Doki, M. Takemoto, T. Ikuta, T. Higuchi, K. Fukui, Y. Usuda, E. Tabuchi, S. Nagatoishi, K. Tsumoto, T. Nishizawa, K. Ito, N. Dohmae, R. Ishitani and O. Nureki
  • Journal Title: Nature Volume: 534 Issue: 7607 Pages: 417-420
  • DOI: 10.1038/nature17991
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Data processing pipeline for serial femtosecond crystallography at SACLA (2016)
  • Author(s): Nakane T, Joti Y, Tono K, Yabashi M, Nango E, Iwata S, Ishitani R, Nureki O
  • Journal Title: J Appl Crystallogr Volume: 49 Issue: 3 Pages: 1035-1041
  • DOI: 10.1107/s1600576716005720
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Structure of Cpf1 in Complex with Guide RNA and Target DNA (2016)
  • Author(s): Yamano T, Nishimasu H, Zetsche B, Hirano H, Slaymaker IM, Li Y, Fedorova I, Nakane T, Makarova KS, Koonin EV, Ishitani R, Zhang F, Nureki O
  • Journal Title: Cell Volume: 165 Issue: 4 Pages: 946-962
  • DOI: 10.1016/j.cell.2016.04.003
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Structural basis for specific inhibition of Autotaxin by a DNA aptamer (2016)
  • Author(s): Kato, K., Ikeda, H., Miyakawa, S., Futakawa, S., Nonaka, Y., Fujiwara, M., Aoki, J., Morita, J., Ishitani, R., Nishimasu, H., Nakamura, Y., Nureki, O.
  • Journal Title: Nature Str. Mol. Biol. Volume: 印刷中 Issue: 5 Pages: 1-9
  • DOI: 10.1038/nsmb.3200
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 3.Mechanisms for Two-Step Proton Transfer Reactions in the Outward-Facing Form of MATE Transporter (2016)
  • Author(s): Wataru Nishima, Wataru Mizukami, Yoshiki Tanaka, Ryuichiro Ishitani, Osamu Nureki, Yuji Sugita
  • Journal Title: Biophys. J Volume: 110 Issue: 6 Pages: 1346-1354
  • DOI: 10.1016/j.bpj.2016.01.027
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Structural Basis for the Altered PAM Specificities of Engineered CRISPR-Cas9 (2016)
  • Author(s): Seiichi Hirano, Hiroshi Nishimasu, Ryuichiro Ishitani, Osamu Nureki
  • Journal Title: Molecular Cell Volume: 61 Issue: 6 Pages: 886-894
  • DOI: 10.1016/j.molcel.2016.02.018
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Structure and biological function of ENPP6, a choline-specific glycerophosphodiester-phosphodiesterase (2016)
  • Author(s): Morita J, Kano K, Kato K, Takita H, Skagami H, Yamamoto Y, Mihara E, Ueda H, Sato T, Tokuyama H, Arai H, Asou H, Takagi J, Ishitani R, Nishimasu H, Nureki O, Aoki J
  • Journal Title: Sci Rep. Volume: 6 Issue: 1 Pages: 20995-20995
  • DOI: 10.1038/srep20995
  • URL: https://localhost/en/publications/d1085467-bb47-4f70-98b0-09d6a10814d8
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Structure and Engineering of Francisella novicida Cas9 (2016)
  • Author(s): Hisato Hirano, Jonathan S. Gootenberg, Takuro Horii, Omar O. Abudayyeh, Mika Kimura, Takanori Nakane, Ryuichiro Ishitani, Izuho Hatada, Feng Zhang, Hiroshi Nishimasu, Osamu Nureki
  • Journal Title: Cell Volume: 164 Issue: 5 Pages: 950-961
  • DOI: 10.1016/j.cell.2016.01.039
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Structural Insights into Divalent Cation Modulations of ATP-Gated P2X Receptor Channels (2016)
  • Author(s): Kasuya, G., Y. Fujiwara, M. Takemoto, N. Dohmae, Y. Nakada-Nakura, R. Ishitani, M. Hattori, and O. Nureki
  • Journal Title: Cell Report Volume: 14 Issue: 4 Pages: 932-944
  • DOI: 10.1016/j.celrep.2015.12.087
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Outward- and inward-facing structures of a putative bacterial transition-metal transporter with homology to ferroportin (2015)
  • Author(s): Taniguchi R, Kato HE, Font J, Deshpande CN, Wada M, Ito K, Ishitani R, Jormakka M, Nureki O
  • Journal Title: Nat. Commun. Volume: 6 Issue: 1 Pages: 8545-8545
  • DOI: 10.1038/ncomms9545
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Crystal Structure of Staphylococcus aureus Cas9 (2015)
  • Author(s): Hiroshi Nishimasu, Le Cong, Winston X. Yan, F. Ann Ran, Bernd Zetsche, Yinqing Li, Arisa Kurabayashi, Ryuichiro Ishitani, Feng Zhang, Osamu Nureki
  • Journal Title: Cell Volume: 162 Issue: 5 Pages: 1113-1126
  • DOI: 10.1016/j.cell.2015.08.007
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Molecular dynamics of channelrhodopsin at the early stages of channel opening (2015)
  • Author(s): Mizuki Takemoto, Hideaki E. Kato, Michio Koyama, Jumpei Ito, Motoshi Kamiya, Shigehiko Hayashi, Andres D. Maturana, Karl Deisseroth, Ryuichiro Ishitani, and Osamu Nureki
  • Journal Title: PLoS ONE Volume: 10 Issue: 6 Pages: 0131094-0131094
  • DOI: 10.1371/journal.pone.0131094
  • NAID: 120005947158
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Atomistic design of microbial opsin-based blue-shifted optogenetics tools (2015)
  • Author(s): Kato HE, Kamiya M, Sugo S, Ito J, Taniguchi R, Orito A, Hirata K, Inutsuka A,Yamanaka A, Maturana AD, Ishitani R, Sudo Y, Hayashi S, Nureki O
  • Journal Title: Nat Commun Volume: 6 Issue: 1 Pages: 7177-7177
  • DOI: 10.1038/ncomms8177
  • NAID: 120005745621
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structural basis for dynamic mechanism of nitrate/nitrite antiport by NarK (2015)
  • Author(s): Fukuda M, Takeda H, Kato HE, Doki S, Ito K, Maturana AD, Ishitani R, Nureki O
  • Journal Title: Nat. Commun. Volume: 6 Issue: 1 Pages: 7097-7097
  • DOI: 10.1038/ncomms8097
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Crystal Structure and Activity of the Endoribonuclease Domain of the piRNA Pathway Factor Maelstrom (2015)
  • Author(s): Naoki Matsumoto, Kaoru Sato, Hiroshi Nishimasu, Yurika Namba, Kana Miyakubi, Naoshi Dohmae, Ryuichiro Ishitani, Haruhiko Siomi, Mikiko C. Siomi, Osamu Nureki
  • Journal Title: Cell Reports Volume: 11 Issue: 3 Pages: 1-10
  • DOI: 10.1016/j.celrep.2015.03.030
  • Peer Reviewed / Open Access
• [Journal Article] Structural Basis for the Catalytic Mechanism of DncV, Bacterial Homolog of Cyclic GMP-AMP Synthase (2015)
  • Author(s): Kazuki Kato, Ryohei Ishii, Seiichi Hirano, Ryuichiro Ishitani, Osamu Nureki
  • Journal Title: Structure Volume: 23 Issue: 5 Pages: 843-850
  • DOI: 10.1016/j.str.2015.01.023
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Structural basis for Na+ transport mechanism by a light-driven Na+ pump (2015)
  • Author(s): H. E. Kato, K. Inoue, R. Abe-Yoshizumi, Y. Kato, H. Ono, M. Konno, S. Hososhima, T. Ishizuka, M. R. Hoque, H. Kunitomo, J. Ito, S. Yoshizawa, K. Yamashita, M. Takemoto, T. Nishizawa, R. Taniguchi, K. Kogure, A. D. Maturana, Y. Iino, H. Yawo, R. Ishitani, H. Kandori*, O. Nureki*
  • Journal Title: Nature Volume: 未定 Issue: 7550 Pages: 48-53
  • DOI: 10.1038/nature14322
  • Peer Reviewed / Open Access
• [Journal Article] Structural basis for the facilitative diffusion mechanism by SemiSWEET transporter (2015)
  • Author(s): Lee Y, Nishizawa T, Yamashita K, Ishitani R, Nureki O
  • Journal Title: Nat Commun Volume: 6 Issue: 1 Pages: 6112-6112
  • DOI: 10.1038/ncomms7112
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Crystal structure of Escherichia coli YidC, a membrane protein chaperone and insertase (2014)
  • Author(s): Kumazaki, K., Kishimoto, T., Furukawa, A., Mori, H., Tanaka, Y., Dohmae, N., Ishitani, R., Tsukazaki, T., and Nureki, O.
  • Journal Title: Scientific reports Volume: 4 Issue: 1 Pages: 7299-7299
  • DOI: 10.1038/srep07299
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Structural basis for ion selectivity revealed by high-resolution crystal structure of Mg(2+) channel MgtE (2014)
  • Author(s): Takeda H, Hattori M, Nishizawa T, Yamashita K, Shah ST, Caffrey M, Maturana AD, Ishitani R, Nureki O
  • Journal Title: Nat Commun Volume: 5 Issue: 1 Pages: 5374-5374
  • DOI: 10.1038/ncomms6374
  • Peer Reviewed / Open Access
• [Journal Article] The non-canonical hydroxylase structure of YfcM reveals a metal ion-coordination motif required for EF-P hydroxylation (2014)
  • Author(s): Kobayashi K, Katz A, Rajkovic A, Ishii R, Branson OE, Freitas MA, Ishitani R, Ibba M, Nureki O
  • Journal Title: Nucleic Acids Res Volume: 42 Issue: 19 Pages: 12295-12305
  • DOI: 10.1093/nar/gku898
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Crystallization and preliminary X-ray crystallographic analysis of YfcM: an important factor for EF-P hydroxylation (2014)
  • Author(s): Kobayashi K, Suzuki T, Dohmae N, Ishitani R, Nureki O
  • Journal Title: Acta Crystallogr F Struct Biol Commun Volume: 70 Issue: 9 Pages: 1236-1239
  • DOI: 10.1107/s2053230x14015726
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Crystallization and preliminary X-ray diffraction analysis of YidC, a membrane-protein chaperone and insertase from Bacillus halodurans. (2014)
  • Author(s): Kumazaki, K., Tsukazaki, T., Nishizawa, T., Tanaka, Y., Kato, H.E., Nakada-Nakura, Y., Hirata, K., Mori, Y., Suga, H., Dohmae, N., Ishitani, R. and Nureki, O.
  • Journal Title: Acta Crystallographica Section F Structural Biology Communications Volume: 70 Issue: 8 Pages: 1056-1060
  • DOI: 10.1107/s2053230x14012540
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Structure of Saccharomyces cerevisiae mitochondrial Qri7 in complex with AMP (2014)
  • Author(s): Tominaga T, Kobayashi K, Ishii R, Ishitani R, Nureki O
  • Journal Title: Acta Crystallogr F Struct Biol Commun Volume: 70 Issue: 8 Pages: 1009-1014
  • DOI: 10.1107/s2053230x14014046
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp6 (2014)
  • Author(s): Morita J, Kato K, Mihara E, Ishitani R, Takagi J, Nishimasu H, Aoki J, Nureki O
  • Journal Title: Acta Crystallogr F Struct Biol Commun Volume: 70 Issue: 6 Pages: 794-799
  • DOI: 10.1107/s2053230x14008929
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Crystal structure of a claudin provides insight into the architecture of tight junctions (2014)
  • Author(s): Suzuki H, Nishizawa T, Tani K, Yamazaki Y, Tamura A, Ishitani R, Dohmae N, Tsukita S, Nureki O, Fujiyoshi Y
  • Journal Title: Science Volume: 344 Issue: 6181 Pages: 304
  • DOI: 10.1126/science.1248571
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Structural basis for Sec-independent membrane protein insertion by YidC. (2014)
  • Author(s): Kumazaki, K., Chiba, S., Takemoto, M., Furukawa, A., Nishiyama, K., Sugano, Y., Mori, T., Dohmae, N., Hirata, K., Nakada-Nakura, Y., Maturana, A.D., Tanaka, Y., Mori, H., Sugita, Y., Arisaka, F., Ito K., Ishitani, R., Tsukazaki, T. and Nureki, O.
  • Journal Title: Nature Volume: 509 Issue: 7501 Pages: 516-520
  • DOI: 10.1038/nature13167
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Crystal structure of Saccharomyces cerevisiae mitochondrial GatFAB reveals a novel subunit assembly in tRNA-dependent amidotransferases (2014)
  • Author(s): Araiso Y, Huot JL, Sekiguchi T, Frechin M, Fischer F, Enkler L, Senger B, Ishitani R, Becker HD, Nureki O
  • Journal Title: Nucleic Acids Res Volume: 42 Issue: 9 Pages: 6052-6063
  • DOI: 10.1093/nar/gku234
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Structural Basis for Potent Inhibition of SIRT2 Deacetylase by a Macrocyclic Peptide Inducing Dynamic Structural Change (2013)
  • Author(s): Yamagata, K., Goto, Y., Nishimasu, H., Morimoto, J., Ishitani, R., Dohmae, N., Takeda, N., Nagai, R., Komuro, I., Suga, H., Nureki, O
  • Journal Title: Structure Volume: 22 Issue: 2 Pages: 345-352
  • DOI: 10.1016/j.str.2013.12.001
  • Peer Reviewed
• [Journal Article] Structural and functional analyses of DNA-sensing and immune activation by human cGAS (2013)
  • Author(s): Kazuki Kato, Ryohei Ishii, Eiji Goto, Ryuichiro Ishitani, Fuminori Tokunaga, and Osamu Nureki
  • Journal Title: PLoS One Volume: 8 Issue: 10 Pages: e76983-e76983
  • DOI: 10.1371/journal.pone.0076983
  • Peer Reviewed
• [Journal Article] Structural basis for dynamic mechanism of proton-coupled symport by the peptide transporter POT. (2013)
  • Author(s): Doki, S., Kato, H. E., Solcan, N., Iwaki, M., Koyama, M., Iwase, N., Tsukazaki, T., Sugita, Y., Kandori, H., Newstead, S., Ishitani, R., and Nureki, O.
  • Journal Title: Proceedings of the National Academy of Sciences of the United States Volume: 110 Issue: 28 Pages: 11343-11348
  • DOI: 10.1073/pnas.1301079110
  • Peer Reviewed
• [Journal Article] Structural Basis for the Counter-Transport Mechanism of a H+/Ca2+ Exchanger (2013)
  • Author(s): Nishizawa T, Kita S, Maturana AD, Furuya N, Hirata K, Kasuya G, Ogasawara S, Dohmae N, Iwamoto T, Ishitani R, Nureki O
  • Journal Title: Science Volume: 341 Issue: 6142 Pages: 168-172
  • DOI: 10.1126/science.1239002
  • Peer Reviewed
• [Journal Article] Screening and X-ray crystal structure-based optimization of autotaxin (ENPP2) inhibitors, using a newly developed fluorescence probe (2013)
  • Author(s): Mitsuyasu Kawaguchi
  • Journal Title: ACS chemical biology Volume: 8 Issue: 8 Pages: 1713-1721
  • DOI: 10.1021/cb400150c
  • Peer Reviewed
• [Journal Article] Structural basis for the drug extrusion mechanism by a MATE multidrug transporter (2013)
  • Author(s): Y・ Tanaka, C・J・ Hipolito, A・D・ Maturana, K・ Ito, T・ Kuroda, T・ Higuchi, T・ Katoh, H・E・ Kato, M・ Hattori, K・ Kumazaki, T・ Tsukazaki, R・ Ishitani, H・ Suga, O・ Nureki
  • Journal Title: Nature Volume: 496 Issue: 7444 Pages: 247-51
  • DOI: 10.1038/nature12014
  • Peer Reviewed
• [Journal Article] Crystal structure of Cex1p reveals the mechanism of tRNA trafficking between nucleus and cytoplasm (2013)
  • Author(s): K. Nozawa, R. Ishitani, T. Yoshihisa, M. Sato, F. Arisaka, S. Kanamaru, N. Dohmae, D. Mangroo, B. Senger, H. D. Becker and O. Nureki
  • Journal Title: Nucleic Acids Research Volume: 41 Issue: 6 Pages: 3901-3914
  • DOI: 10.1093/nar/gkt010
  • Peer Reviewed
• [Presentation] Structural basis for amino acid export by DMT superfamily transporter YddG (2016)
  • Author(s): Ryuichiro Ishitani
  • Organizer: GRC Membrane Transport Proteins
  • Place of Presentation: Lucca (Barga), Italy
  • Year and Date: 2016-06-12
  • Int'l Joint Research
• [Presentation] 膜輸送体の分子メカニズム (2016)
  • Author(s): 石谷隆一郎
  • Organizer: 分子生物学会第３９回年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Int'l Joint Research / Invited
• [Presentation] Molecular Mechanisms of Membrane Transporters (2015)
  • Author(s): Ryuichiro Ishitani
  • Organizer: The 13th Conference of the Asian Crystallographic Association
  • Place of Presentation: Science City, Kolkata, India
  • Year and Date: 2015-12-05
  • Int'l Joint Research / Invited
• [Presentation] Structural Basis for Dynamic Mechanism of Nitrate/Nitrite Antiport by NarK (2015)
  • Author(s): Ryuichiro Ishitani
  • Organizer: GRC Mechanisms of Membrane Transport
  • Place of Presentation: Bates College, Lewiston, ME, USA
  • Year and Date: 2015-06-28
  • Int'l Joint Research
• [Presentation] プロトンで駆動されるトランスポーターの分子機構 (2015)
  • Author(s): 石谷隆一郎
  • Organizer: 分子研研究会「膜タンパク質内部のプロトン透過を考える」
  • Place of Presentation: 岡崎コンファレンスセンター 愛知県岡崎市明大寺町伝馬８－１
  • Year and Date: 2015-04-20
  • Invited
• [Presentation] 先端放射光を用いたチャネル・トランスポーターの高分解能構造解析と分子機構の解明 (2015)
  • Author(s): 石谷隆一郎
  • Organizer: 日本放射光学会
  • Place of Presentation: 立命館大学びわこ･くさつキャンパス
  • Year and Date: 2015-01-11
  • Invited
• [Presentation] 炎症応答に関わる因子とその機能をモデュレートする化合物の構造生物学 ― Cyclic GMP-AMP合成酵素の結晶構造 ― (2014)
  • Author(s): 石谷隆一郎
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜会議センター
  • Year and Date: 2014-11-25
  • Invited
• [Presentation] 炎症応答に関わる因子の構造生物学 Cyclic GMP-AMP合成酵素の結晶構造 (2014)
  • Author(s): 石谷隆一郎
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2014-10-16
  • Invited
• [Presentation] Crystal structure of channelrhodopsin ― a light-gated cation channel ― (2014)
  • Author(s): 石谷隆一郎
  • Organizer: 16th International Congress on Photobiology
  • Place of Presentation: Universidad Nacional de Cordoba, Cordoba, Argentina
  • Year and Date: 2014-09-08
  • Invited
• [Presentation] シグナル伝達にかかわる因子Autotaxinの構造ベース創薬 (2014)
  • Author(s): 石谷隆一郎
  • Organizer: 日本薬学会
  • Place of Presentation: 熊本大学（熊本県）
  • Invited
• [Presentation] Crystal structure of YidC reveals a membrane protein insertion mechanism (2014)
  • Author(s): Ryuichiro Ishitani
  • Organizer: Gordon Research Conference on Ligand recognition & molecular gating
  • Place of Presentation: Ventura CA, USA
• [Presentation] Structural basis for mRNA surveillance by archaeal Pelota and GTP-bound EF1α complex (2013)
  • Author(s): Ryuichiro Ishitani
  • Organizer: EMBO Conference "Eukaryotic RNA Turnover: From Structural Insights to Diseases"
  • Place of Presentation: Strasbourg, France
• [Presentation] 最先端のマイクロビームラインと計算機シミュレーションを用いた膜輸送体の構造生物学 (2013)
  • Author(s): 石谷隆一郎
  • Organizer: 蛋白質科学会
  • Place of Presentation: とりぎん文化会館（鳥取県）
  • Invited