| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2015: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Outline of Final Research Achievements |
SR calcium uptake is mediated by a SERCA2a, whose activity is reversibly regulated by its direct mediator phospholamban (PLN). PLN is the most important mediator of β adrenergic signal, and it controls SERCA2a depending on its phosphorylation state. Recently many regulators that controls the PLN/SERCA2a complex was discovered. They are thought to form a large-scale complex (PLN/SERCAa regulatome). However, due to lack of efficient expression/purification system for SERCA2/PLN, the details of the mechanisms how they regulate PLN/SERCAa complex is unclear. Thus, we aimed to elucidate the mechanism of regulation of PLN/SERCA2a by regulators with using our own large expression and purification system.
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