| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
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| Outline of Final Research Achievements |
Anammox bacteria produce dinitorgen from nitrite and ammonium under anaerobic condition. The key enzyme of nitorogen metabolism of anammox is hydrazine synthase, which produces hydrazine from nitric oxide and anmmonium. In this study, we have prepared 10-L scale membrane bioreactor for cultivation of anammox bacteria. Large amount of HZS was extracted from anammox bacteria and highly purified HZS are obtained by anion exchange and hydroxyapatite chromatography. We have also cloned HZS gene (hzsABC) from anammox bacteria genome and constructed monocistronic and polycistronic expression vector of HZS. Although some of HZS A subunit lost heme c, we have confirmed expression of HZS in E. coli.
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