The mechanism analysis of influenza RNA polymerase
| Project/Area Number |
25291016
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Yokohama City University |
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Principal Investigator |
Park Sam-Yong 横浜市立大学, 生命医科学研究科, 教授 (20291932)
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| Co-Investigator(Kenkyū-buntansha) |
Sugiyama Kanako 公益財団法人神奈川科学技術アカデミー, 革新的インフルエンザウイルス創薬プロジェクト, 研究員 (20623226)
Urano Takeshi 島根大学, 医学部, 教授 (70293701)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2014: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2013: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
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| Keywords | インフルエンザ / RNAポリメラーゼ / 抗体 / 構造解析 / 創薬 |
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| Outline of Final Research Achievements |
Influenza RNA polymerase plays vital roles in the virus life cycle and, since it is much more highly conserved than other influenza virus proteins, it is of great interest as a drug target. This laboratory has created monoclonal antibodies that bind to fragments of the polymerase and block viral replication. Antibody PA11.9 binds to the PA subunit carrying the RNA polymerisation active site.
Two other antibodies were shown to bind to the RNA-binding PB2 subunit of the polymerase, including antibody PB2 3-1.6, which has notably high affinity for its target. This antibody has been patented as a research tool and diagnostic reagent (patent number 2014-068824).
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] A novel 3' splice site recognition by the two zinc fingers in the U2AF small subunit.2015
Author(s)
Yoshida H, Park S-Y, Oda T, Akiyoshi T, Sato M, Shirouzu M, Tsuda K, Kuwasako K, Unzai S, Muto Y, Urano T, Obayashi E.
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Journal Title
Genes Dev.
Volume: 29
Issue: 15
Pages: 1649-1660
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] A Novel Tricyclic Polyketide, Vanitaracin A, Specifically Inhibits the Entry of Hepatitis B and D Viruses by Targeting Sodium Taurocholate Cotransporting Polypeptide2015
Author(s)
Kaneko M, Watashi K, Kamisuki S, Matsunaga H, Iwamoto M, Kawai F, Ohashi H, Tsukuda S, Shimura S, Suzuki R, Aizaki H, Sugiyama M, Park SY, Ito T, Ohtani N, Sugawara F, Tanaka Y, Mizokami M, Sureau C, Wakita T
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Journal Title
J Virol
Volume: 89
Issue: 23
Pages: 11945-11953
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] The crystal structure of the active domain of Anopheles anti-platelet protein, a powerful anti-coagulant, in complex with an antibody.2014
Author(s)
Sugiyama, K., Iyori, M., Sawaguchi, A., Akashi, S., Tame, J.R.H., Park, S.-Y., Yoshida, S.
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Journal Title
J. Biol. Chem.
Volume: 289
Issue: 23
Pages: 16303-16312
DOI
Related Report
Peer Reviewed / Open Access
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