Structural and functional analysis of CENP-TWSX complex
| Project/Area Number |
25291018
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Tokyo University of Science (2016)
National Institute of Genetics (2013-2015) |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
FUKAGAWA Tatsuo 大阪大学, 生命機能研究科, 教授 (60321600)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2015: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Keywords | X線結晶構造解析 / 染色体分配 / タンパク質複合体 / 生化学 / 構造生物学 / 酵素反応 / 生物物理 / 分子認識 / 蛋白質 |
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| Outline of Final Research Achievements |
Replicated sister chromatids are faithfully segregated into two daughter cells. Kinetochore is built at the centromeric region of the chromosome and is involved in the chromosome segregation. CENP-TWSX complex is a conserved kinetochore component and plays an important role. It contains histone fold and binds to DNA but its precise mechanism remains elusive. To elucidate the structure and function of the CENP-TWSX complex, we have undertaken biochemical and structural analyses. Using isothermal titration calorimetry, we obtained dissociation constant of the CENP-TW and CENP-SX interaction. Next, we measured interaction between DNA and CENP-TW or CENP-SX. Using gel filtration, we acquired the stoichiometry of the complex. Dissociation constants were measured by the fluorescent anisotrophy method. Furthermore, we obtained crystal structure of the FANC-M-CENP-SX complex. Together, these analyses revealed detailed molecular mechanism of the CENP-TWSX complex.
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Report
(5 results)
Research Products
(25 results)
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[Presentation] 好熱性真核生物の朝明2017
Author(s)
西野 達哉
Organizer
第5回蛋白質相互作用研究会
Place of Presentation
静岡県御殿場市・時之栖ホテルBrush-up
Year and Date
2017-03-28
Related Report
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[Presentation] ヒトHP1の構造機能解析2017
Author(s)
佐久間 佳織、迫 洸佑、広田 亨、西野 達哉
Organizer
2016年度量子ビームサイエンスフェスタ
Place of Presentation
茨城県つくば市、つくば国際会議場
Year and Date
2017-03-14
Related Report
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