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Study of functional interplay of tRNA mimicry proteins and translational GTPase proteins.

Research Project

Project/Area Number 25291020
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Functional biochemistry
Research InstitutionThe University of Tokyo

Principal Investigator

Ito Koichi  東京大学, 新領域創成科学研究科, 教授 (10262073)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2015: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Keywordsリボソーム / 翻訳制御 / 遺伝暗号 / 直行性 / タンパク質合成 / tRNA擬態タンパク質 / 翻訳終結 / mRNA品質管理 / 直交性
Outline of Final Research Achievements

We performed genetic mapping and analyses of critical sites that contribute to functional complementation with exceptionally nonfunctional heterologous eRF3 in yeast, and clarified the functional interplay among the tRNA mimic eRF1, its carrier protein eRF3 and ribosome.
To elucidate the molecular roles of this residue in codon discrimination, we conducted systematic mutagenesis of 123rd amino acid and applied to stop codon specificity analyses in vivo. We found that a simple rule could explain sub-specific patterns of 123th residues in codon discrimination, and that the residue contributed to functional coupling with eRF3, suggesting that the 123th residue is a novel class of functional residue. Furthermore, our structural modeling of eRF1 in the quasi-A/T state also suggested a distinctive role for the 123th residue in the putative molecular interplays on the ribosomal decoding site. We also proposed a model for the putative role of the 123th residue in stop codon discrimination.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (12 results)

All 2015 2014 2013

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (8 results)

  • [Journal Article] Inhibiting K63 Polyubiquitination Abolishes No-Go Type Stalled Translation Surveillance in Saccharomyces cerevisiae2015

    • Author(s)
      Kazuki Saito, Wataru Horikawa, Koichi Ito
    • Journal Title

      PLOS Genetics

      Volume: in press Issue: 4 Pages: e1005197-e1005197

    • DOI

      10.1371/journal.pgen.1005197

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Genetic analysis of L123 of the tRNA-mimicking eukaryote release factor eRF1, an amino acid residue critical for discrimination of stop codons2015

    • Author(s)
      Kazuki Saito, Koichi Ito
    • Journal Title

      Nucleic Acid Research

      Volume: in press Issue: 9 Pages: 4591-4601

    • DOI

      10.1093/nar/gkv376

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Cell growth control by stable Rbg2/Gir2 complex formation under amino acid starvation.2013

    • Author(s)
      Ishikawa, K
    • Journal Title

      Genes Cells

      Volume: 18 Issue: 10 Pages: 859-872

    • DOI

      10.1111/gtc.12082

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Single Amino Acid Substitution Alters Omnipotent eRF1 of Dileptus to Euplotes-type Dualpotent eRF12013

    • Author(s)
      Li Y, Kim OT, Ito K, Saito K, Suzaki T,Harumoto T.
    • Journal Title

      Standard Codon Usage May be Advantageous in Raptorial Ciliates. Protist.

      Volume: 164 Issue: 3 Pages: 440-449

    • DOI

      10.1016/j.protis.2013.02.004

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] eIF4Aファミリータンパク質の機能性ドメインの互換性検証2015

    • Author(s)
      奥村 知之、田村 浩二、伊藤 耕一
    • Organizer
      第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] tRNA擬態タンパク質による遺伝暗号解読システムの拡張2015

    • Author(s)
      伊藤 耕一
    • Organizer
      第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] Role of polyubiquitination specific for quality control of stalled translation2014

    • Author(s)
      Kazuki Saito, Wataru Horikawa, Koichi Ito
    • Organizer
      EMBL Symposia: The Complex Life of mRNA
    • Place of Presentation
      Heidelberg (Germany)
    • Year and Date
      2014-10-05 – 2014-10-08
    • Related Report
      2014 Annual Research Report
  • [Presentation] 真核生物翻訳終結複合体eRF1/eRF3の協調的機能領域の新規同定2014

    • Author(s)
      和田美紀、伊藤耕一
    • Organizer
      第16回日本RNA学会年会
    • Place of Presentation
      ウインクあいち(愛知県名古屋市)
    • Year and Date
      2014-07-23 – 2014-07-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] 伸長因子EF-1alphaホモログGタンパク質Ski7の変異体解析2014

    • Author(s)
      堀川航、伊藤耕一
    • Organizer
      第16回日本RNA学会年会
    • Place of Presentation
      ウインクあいち(愛知県名古屋市)
    • Year and Date
      2014-07-23 – 2014-07-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] 終止コドン解読におけるeRF1とeRF3の共役の解析2013

    • Author(s)
      斎藤和紀、伊藤耕一
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2013 Annual Research Report
  • [Presentation] 出芽酵母におけるEF-1αホモログGタンパク質Ski7の機能ドメイン解析2013

    • Author(s)
      堀川航、和田美紀、伊藤耕一
    • Organizer
      第15回日本RNA学会年会
    • Place of Presentation
      愛媛県民文化会館・ひめぎんホール 松山市道後
    • Related Report
      2013 Annual Research Report
  • [Presentation] 古細菌由来 aRF1-aEF1α-GTP 複合体による翻訳終結機構の 構造基盤2013

    • Author(s)
      小林 幹, 斎藤 和紀, 石谷 隆一郎, 伊藤 耕一, 濡木 理
    • Organizer
      第15回日本RNA学会年会
    • Place of Presentation
      愛媛県民文化会館・ひめぎんホール 松山市道後
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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