| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2015: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Outline of Final Research Achievements |
We performed genetic mapping and analyses of critical sites that contribute to functional complementation with exceptionally nonfunctional heterologous eRF3 in yeast, and clarified the functional interplay among the tRNA mimic eRF1, its carrier protein eRF3 and ribosome.
To elucidate the molecular roles of this residue in codon discrimination, we conducted systematic mutagenesis of 123rd amino acid and applied to stop codon specificity analyses in vivo. We found that a simple rule could explain sub-specific patterns of 123th residues in codon discrimination, and that the residue contributed to functional coupling with eRF3, suggesting that the 123th residue is a novel class of functional residue. Furthermore, our structural modeling of eRF1 in the quasi-A/T state also suggested a distinctive role for the 123th residue in the putative molecular interplays on the ribosomal decoding site. We also proposed a model for the putative role of the 123th residue in stop codon discrimination.
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