Identificatin and characterization of bactrial genes for coexistence with hosts
| Project/Area Number |
25291021
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SHIRATSUCHI Akiko 金沢大学, 医薬保健研究域薬学系, 准教授 (90303297)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
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| Keywords | 細菌感染症 / 自然免疫 / ショウジョウバエ / 大腸菌 / 遺伝子発現制御 / 宿主と病原体の相互作用 / 二成分制御系 |
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| Outline of Final Research Achievements |
Bacterial infection often causes serious diseases. This could be avoided when the virulence of bacteria is kept sufficiently low. We hypothesize that bacteria possess a gene(s) that creates the coexistence of hosts and invading bacteria. This study was undertaken to identify such a bacterial gene using Escherichia coli and Drosophila melanogaster as a model bacterium and host, respectively. We identified envZ-ompR, a pair of genes coding for the two-component regulatory system EnvZ-OmpR, that mitigates the virulence of E. coli in Drosophila. The enhanced expression of envZ-ompR and the phosphorylation of EnvZ were observed under infectious state. Forced expression of ompC, one of the target genes of EnvZ-OmpR, rescued the virulence phenotype of an envZ-ompR mutant. OmpC as an outer membrane protein has been known to protect bacteria from a change of osmotic pressure. The mechanisms of the induction of envZ-ompR and a mode of actions of OmpC await further investigation.
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] Peptidoglycan recognition protein-triggered induction of Escherichia coli gene in Drosophila melanogaster2015
Author(s)
Kong, Q., Nakai, Y., Kuroda, N., Shiratsuchi, A., Nagaosa, K., and Nakanishi, Y.
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Journal Title
J. Biochem.
Volume: 00
Issue: 6
Pages: 00-00
DOI
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Role for σ38 in prolonged survival of Escherichia coli in Drosophila melanogaster.2014
Author(s)
Shiratsuchi, A., Shimamoto, N., Nitta, M., Tuan, T. Q., Firdausi, A., Gawasawa, M., Yamamoto, K., Ishihama, A., and Nakanishi, Y.
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Journal Title
J. Immunol.
Volume: 192
Issue: 2
Pages: 666-675
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Involvement of EnvZ-OmpR two-component system in virulence control of Escherichia coli in Drosophila melanogaster.2013
Author(s)
Pullkay, P., Nakanishi, Y, Nitta, M., Yamamoto, K., Ishihama, A., and Shiratsuchi, A.
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Journal Title
Biochem. Biophys. Res. Commun.
Volume: 438
Issue: 2
Pages: 306-311
DOI
Related Report
Peer Reviewed
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