Studies on the sperm extracellular ubiquitin-proteasome system involved in fertilization
| Project/Area Number |
25291024
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
Sawada Hitoshi 名古屋大学, 理学(系)研究科(研究院), 教授 (60158946)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2015: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2014: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Keywords | 受精 / ユビキチン / プロテアソーム / 精子 / 細胞外 / プロテアーゼ / ライシン / ホヤ / ユビキチン化酵素 |
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| Outline of Final Research Achievements |
We previously reported that the extracellular ubiquitin-proteasome system may be involved in fertilization of the ascidian Halocynthia roretzi. Here, we cloned two cDNAs of ubiquitin-activating enzymes (E1), HrUBA1 and HrUBA6 and showed that these mRNAs are expressed in most organs, including the ovary and testis. Immunocytochemistry using peptide antibodies revealed that these enzymes are localized on follicle cells of the eggs and on sperm head around a mitochondrion. E1 inhibitor PYR-41 inhibited fertilization, suggesting that gamete E1s may be involved in fertilization. We also studied on the sperm proteasome by using a peptide antibody against sperm proteasome. The results showed that the sperm proteasome is localized on the surface of sperm head over a mitochondrion. Since anti-proteasome antibodies inhibited fertilization, it was suggested that the sperm proteasome plays a key extracellular role in fertilization.
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Report
(5 results)
Research Products
(67 results)
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[Journal Article] Transcriptional Framework of Male Gametogenesis in the Liverwort Marchantia polymorpha L.2016
Author(s)
Asuka Higo, Masaki Niwa, Katsuyuki T. Yamato, Lixy Yamada, Hitoshi Sawada, Tomoaki Sakamoto, Tetsuya Kurata, Makoto Shirakawa, Motomu Endo, Shuji Shigenobu, Katsushi Yamaguchi, Kimitsune Ishizaki, Ryuichi Nishihama, Takayuki Kohchi, and Takashi Araki
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Journal Title
Plant and Cell Physiology
Volume: 57(2)
Issue: 2
Pages: 325-338
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] ANISEED 2015: a digital framework for the comparative developmental biology of ascidians.2015
Author(s)
Brozovic, Martin, Dantec, Dauga, Mendez, Simion, Percher, Laporte, Scornavacca, Di Gregorio, Fujiwara, et al.
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Journal Title
Nucleic Acids Research
Volume: 44
Issue: D1
Pages: 808-818
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Self-incompatibility in the ascidian, Ciona intestinalis: Possible roles of s/v-Themis-A, B, B2 and Themis-like in gamete self-recognition2016
Author(s)
Hitoshi Sawada, Kei Otsuka, Arata Higuchi, Kazunori Yamamoto, Akira Yamaguchi, Lixy Yamada, Yoshito Harada, Masashi Fukuoka, Shiori Nakazawa, Maki Shirae-Kurabayashi
Organizer
The 22nd International Congress of Zoology
Place of Presentation
沖縄
Year and Date
2016-11-14
Related Report
Int'l Joint Research
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