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Roles of syntaxin 17 in the formation and function of the mitochondria-associated ER membrane

Research Project

Project/Area Number 25291029
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Functional biochemistry
Research InstitutionTokyo University of Pharmacy and Life Science

Principal Investigator

TAGAYA MITSUO  東京薬科大学, 生命科学部, 教授 (30179569)

Co-Investigator(Kenkyū-buntansha) INOUE Hiroki  東京薬科大学, 生命科学部, 講師 (10294448)
HASHIMOTO Yoshitami  東京薬科大学, 生命科学部, 助教 (50616761)
ARASAKI Kohei  東京薬科大学, 生命科学部, 講師 (70609990)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Keywords小胞体 / ミトコンドリア / syntaxin17 / レジオネラ / 神経変性疾患
Outline of Final Research Achievements

The MAM(mitochondria-associated membrane)is one of the subdomains of the ER and participates in various cellular phenomena. We found that syntaxin 17 (Syn17) is localized in the MAM and plays the following functions: (1) Syn17 promotes mitochondrial fission by defining the localization and activity of the mitochondrial fission factor Drp1. (2) Syn17 regulates Ca2+ homeostasis of the ER. (3) Syn17 regulates the localization of PGAM5, a protein phosphatase for Drp1. (4) MAP1B-LC1 mediates the link of Syn17 with microtubules and Drp1. (5) Upon infection, Legionella degrades Syn17, and one of its effectors is responsible for this degradation.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (26 results)

All 2015 2014 2013 Other

All Journal Article (9 results) (of which Peer Reviewed: 6 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (15 results) (of which Invited: 3 results) Remarks (2 results)

  • [Journal Article] γ-SNAP stimulates disassembly of endosomal SNARE complexes and regulates endocytic trafficking pathways.2015

    • Author(s)
      Inoue, H., Matsuzaki, Y., Tanaka, A., Hosoi, K., Ichimura, K., Arasaki, K., Wakana, Y., Asano, K., Tanaka, M., Okuzaki, D., Yamamoto, A., Tani, K. and Tagaya, M.
    • Journal Title

      J. Cell Sci.

      Volume: 128 Pages: 2781-2794

    • DOI

      10.1242/jcs.158634

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 変わりゆくオルガネラ間コミュニケーションの概念2015

    • Author(s)
      多賀谷光男
    • Journal Title

      実験医学

      Volume: 33 Pages: 2546-2552

    • Related Report
      2015 Annual Research Report
  • [Journal Article] MAM-ミトコンドリア機能を制御する小胞体サブドメイン2015

    • Author(s)
      多賀谷光男、新崎恒平、谷佳津子
    • Journal Title

      実験医学

      Volume: 33 Pages: 2553-2559

    • Related Report
      2015 Annual Research Report
  • [Journal Article] A Role for the Ancient SNARE Syntaxin 17 in Regulating Mitochondrial Division2015

    • Author(s)
      K. Arasaki, H. Shimizu, H. Mogari, N. Nishida, N. Hirota, A. Furuno, Y. Kudo, M. Baba, N. Baba, J. Cheng, T. Fujimoto, N.Ishihara, C. Ortiz-Sandoval, L. Barlow, A Raturi, N. Dohmae, Y. Wakana, H. Inoue, K.Tani, J. Dacks, T.Simmen, M. Tagaya
    • Journal Title

      Developmental Cell

      Volume: 32(9) Issue: 3 Pages: 304-317

    • DOI

      10.1016/j.devcel.2014.12.011

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Valosin-containing protein-interacting membrane protein (VIMP) links the endoplasmic reticulum with microtubules in concert with cytoskeleton-linking membrane protein (CLIMP)-632014

    • Author(s)
      Noda, C., Kimura, H., Arasaki, K., Matsushita, M., Yamamoto, A., Wakana, Y., Inoue, H., and Tagaya, M.
    • Journal Title

      J. Biol. Chem.

      Volume: 289 Issue: 35 Pages: 24304-24313

    • DOI

      10.1074/jbc.m114.571372

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Moonlighting functions of the NRZ (mammalian Dsl1) complex. Front2014

    • Author(s)
      Tagaya, M., Arasaki, K., Inoue, H., and Kimura, H.
    • Journal Title

      Cell Develop. Biol

      Volume: (in press) Pages: 25-25

    • DOI

      10.3389/fcell.2014.00025

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] MAMの新たな機能と関連疾患2014

    • Author(s)
      2. 多賀谷光男、若菜裕一、川端美緒
    • Journal Title

      細胞工学

      Volume: 33 Pages: 436-442

    • NAID

      40020031737

    • Related Report
      2013 Annual Research Report
  • [Journal Article] PtdIns(3)P-bound UVRAG coordinates Golgi-ER retrograde and Atg9 transport by differential interactions with the ER tether and the Beclin1 complex.2013

    • Author(s)
      He, S., Ni, D., Ma, B., Lee, J.-H., Zhang, T., Ghozalli, I., Pirooz, S. D., Zhao, Z., Bharatham, N., Li, B., Oh, S., Lee, W.-H., Takahashi, Y., Wang, H.-G., Minassian, A., Feng, P., Deretic, V., Pepperkok, R., Tagaya, M., Yoon, H. S., and Liang, C.
    • Journal Title

      Nat. Cell Biol.

      Volume: 15 Issue: 10 Pages: 1206-1219

    • DOI

      10.1038/ncb2848

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A new role for RINT-1 in SNARE complex assembly at the trans-Golgi network in coordination with the COG complex.2013

    • Author(s)
      A new role for RINT-1 in SNARE complex assembly at the trans-Golgi network in coordination with the COG complex.
    • Journal Title

      Mol Biol Cell

      Volume: 24 Issue: 18 Pages: 2907-2917

    • DOI

      10.1091/mbc.e13-01-0014

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] 脂肪滴の生合成形におけるsyntaxin 17の関与2015

    • Author(s)
      木村葉那、新崎恒平、多賀谷光男
    • Organizer
      第38回日本分子生物学会年会-第88回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] MAP1B-LC1はsyntaxin17との相互作用を介してオートファジーを制御する2015

    • Author(s)
      長島晴輝、新崎恒平、黒澤優里、堂前直、多賀谷光男
    • Organizer
      第38回日本分子生物学会年会-第88回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] Regulation of mitochondrial division and mitophagy by syntaxin 172015

    • Author(s)
      Kohei Arasaki, Masashi Sugo, Naohiko Hirota, Naoshi Dohmae, Carolina Ortiz-Sandoval, Thomas Simmen, Yuzuru Imai, Nobutaka Hattori, and Mitsuo Tagaya
    • Organizer
      Mutifaceted Mitochondria
    • Place of Presentation
      Chicago
    • Year and Date
      2015-07-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] Syntaxin17はDrp1の脱リン酸化酵素であるPGAM5の局在を調節する2015

    • Author(s)
      須合真士、新崎恒平、堂前直、多賀谷光男
    • Organizer
      第67回日本細胞生物学会大会
    • Place of Presentation
      東京
    • Year and Date
      2015-06-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] Syntaxin17の分解に関わるレジオネラエフェクターの探索2015

    • Author(s)
      三上優美、ハーベイ ジェームス、ロイ クレイグ、多賀谷光男、新崎恒平
    • Organizer
      第67回日本細胞生物学会大会
    • Place of Presentation
      東京
    • Year and Date
      2015-06-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] レジオネラ感染に関わるRabタンパク質の同定及びレジオネラ菌の小胞体定着化機構2015

    • Author(s)
      新崎恒平、川端美緒、加藤郁子、多賀谷光男
    • Organizer
      第88回日本細菌学会総会
    • Place of Presentation
      岐阜
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] レジオネラ菌によるRab5ユビキチン化機構の解明2015

    • Author(s)
      川端美緒、加藤郁子、多賀谷光男、新崎恒平
    • Organizer
      第88回日本細菌学会総会
    • Place of Presentation
      岐阜
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] レジオネラ菌は小胞体内での移行にRab4及びRab10を利用する2015

    • Author(s)
      加藤郁子、川端美緒、新崎恒平、多賀谷光男
    • Organizer
      第88回日本細菌学会総会
    • Place of Presentation
      岐阜
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] レジオネラ菌の宿主小胞体への移行及び定着化の機構2014

    • Author(s)
      新崎恒平、川端美緒、野内優、多賀谷光男
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] PGAM5によるDrp1の脱リン酸化へのSyntaxin17の関与2014

    • Author(s)
      須合真士、新崎恒平、堂前直、多賀谷光男
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] レジオネラ菌の小胞体への侵入にはRab6が機能する2014

    • Author(s)
      川端美緒、加藤郁子、新崎恒平
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] Rab4及びRab10のレジオネラ感染における機能解析2014

    • Author(s)
      加藤郁子、川端美緒、新崎恒平
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] RINT-1の新たな役割:COG複合体との協調によるTGN局在SNARE複合体の会合の調節2013

    • Author(s)
      新崎恒平、高木大地、古野暁子、相田美和、三角佳生、多賀谷光男
    • Organizer
      第65回日本細胞生物学会大会
    • Place of Presentation
      名古屋
    • Related Report
      2013 Annual Research Report
  • [Presentation] 小胞体とミトコンドリアの接触部位におけるsyntaxin17の役割2013

    • Author(s)
      多賀谷光男、新崎恒平
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 浸潤突起形成に関わるSNAREタンパク質の探索2013

    • Author(s)
      青木瑶子、渡邉卓也、多賀谷光男、井上弘樹
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Remarks]

    • URL

      http://pathos.ls.toyaku.ac.jp/?page_id=121

    • Related Report
      2014 Annual Research Report
  • [Remarks] 分子細胞生物学研究室のページ

    • URL

      http://pathos.ls.toyaku.ac.jp/?page_id=121

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2013-05-21   Modified: 2019-07-29  

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