Clarification of molecular mechanism for the catabolism of glycans on glycoproteins in budding yeast

• Project/Area Number: 25291030
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Tadashi Suzuki 国立研究開発法人理化学研究所, 糖鎖代謝学研究チーム, チームリーダー (90345265)
• Co-Investigator(Renkei-kenkyūsha): HARADA Yoichiro 国立研究開発法人理化学研究所, 糖鎖代謝学研究チーム, 特別研究員 (80464147) ; HIRAYAMA Hiroto 国立研究開発法人理化学研究所, 糖鎖代謝学研究チーム, 協力研究員 (50525847)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2013: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
• Keywords: 糖鎖代謝 / 出芽酵母 / N型糖鎖 / PNGase / オリゴ糖転移酵素 / O-マンノース / O型糖鎖 / 糖タンパク質 / 糖鎖 / 代謝 / 遊離糖鎖

Outline of Final Research Achievements

This study aimed at clarifying the catbaolic mechanism of glycans on glycoproteins in budding yeast. We found that the cytosolic PNGase-indepedent formation of free N-glycans were mediated by oligosaccharyltransferase. We also found lack of evidence for the occurrence of catabolic alpha-glucosidase/beta-mannosidase/beta-hexosaminidase in this yeast. We also identified several endogenous substrate for the cytoplasmic PNGase involved in the catabolism of glycoproteins.

Research Products

• [Journal Article] Lack of the evidence for the enzymatic catabolism of Man1GlcNAc2 in Saccharomyces cerevisiae. (2016)
  • Author(s): Tanim Jabid Hossain, Hiroto Hirayama, Yoichiro Harada and Tadashi Suzuki
  • Journal Title: Bioscience, Biotechnology and Biochemistry Volume: 80 Issue: 1 Pages: 152-157
  • DOI: 10.1080/09168451.2015.1072464
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] tructural analysis of free N-glycans in alpha-glucosidase mutants of Saccharomyces cerevisiae. Lack of the evidence for the occurrence of catabolic a-glucosidase acting on the N-glycans. (2016)
  • Author(s): Tanim Jabid Hossain, Yoichiro Harada, Hiroto Hirayama, Haruna Tomotake, Akira Seko and Tadashi Suzuki
  • Journal Title: PLoS One Volume: 11 Issue: 3 Pages: e0151891-e0151891
  • DOI: 10.1371/journal.pone.0151891
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cytoplasmic peptide:N-glycanase removes N-glycans on a carboxypeptidase mutant in Saccharomyces cerevisiae. (2015)
  • Author(s): Akira Hosomi and Tadashi Suzuki
  • Journal Title: Biochim. Biophys. Acta Volume: 1850 Issue: 4 Pages: 612-619
  • DOI: 10.1016/j.bbagen.2014.12.008
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Physiological and molecular functions of the cytosolic peptide:N-glycanase. (2015)
  • Author(s): Hiroto Hirayama, Akira Hosomi, and Tadashi Suzuki
  • Journal Title: Semin Cell Dev Biol. Volume: NA Pages: 110-120
  • DOI: 10.1016/j.semcdb.2014.11.009
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Generation and degradation of free asparagine-linked glycans. (2015)
  • Author(s): Yoichiro Harada, Hiroto Hirayama, and Tadashi Suzuki
  • Journal Title: Cell Mol. Life Sci. Volume: NA Issue: 13 Pages: 2509-2533
  • DOI: 10.1007/s00018-015-1881-7
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Non-lysosomal degradation pathway for N-linked glycans and dolichol linked oligosaccharides (2014)
  • Author(s): Tadashi Suzuki and Yoichiro Harada
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 453 Issue: 2 Pages: 213-219
  • DOI: 10.1016/j.bbrc.2014.05.075
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Metabolically programmed quality control system for dolichol-linked oligosaccharides (2013)
  • Author(s): Harada Y, Nakajima K, Masahara-Negishi Y, Freeze HH, Angata T, Taniguchi N, Suzuki T
  • Journal Title: Proc Natl Acad Sci U S A Volume: 110 Issue: 48 Pages: 19366-19371
  • DOI: 10.1073/pnas.1312187110
  • Peer Reviewed
• [Journal Article] Eukaryotic oligosaccharyltransferase generates free oligosaccharides during N-glycosylation (2013)
  • Author(s): Harada Y, Buser R, Ngwa EM, Hirayama H, Aebi M, Suzuki T
  • Journal Title: J Biol Chem Volume: 288 Issue: 45 Pages: 32673-32684
  • DOI: 10.1074/jbc.m113.486985
  • Peer Reviewed
• [Presentation] Functional analysis of the generation of novel forms of free-glycans. (2016)
  • Author(s): Tadashi Suzuki and Hiroto Hirayama
  • Organizer: 5th RIKEN-MPG Joint Symposium on Chemical Biology at Berlin
  • Place of Presentation: Harnack-Haus, Berlin (Germany)
  • Year and Date: 2016-04-17
  • Int'l Joint Research
• [Presentation] Analysis of the catabolic pathway of N-glycans in S. cerevisiae (2014)
  • Author(s): Tanim Jabid Hossain, Hiroto Hirayama, Yoichiro Harada and Tadashi Suzuki
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25
• [Presentation] Analysis of the catabolic pathway of N-glycans in S. cerevisiae. (2014)
  • Author(s): Tanim Jabid Hossain, Hiroto Hirayama, Yoichiro Harada and Tadashi Suzuki
  • Organizer: GlycoTOKYO2014
  • Place of Presentation: 千葉大学
  • Year and Date: 2014-11-04
• [Presentation] 遊離糖鎖生成に関わる新規因子の探索 (2014)
  • Author(s): 平山 弘人、鈴木 匡
  • Organizer: 第47回酵母遺伝学フォーラム
  • Place of Presentation: 東京大学
  • Year and Date: 2014-09-01
• [Presentation] Role of oligosaccharyltransferase on PNGase-independent release of free oligosaccharides. (2014)
  • Author(s): Yoichiro Harada and Tadashi Suzuki (invited speaker)
  • Organizer: GlycoT2014
  • Place of Presentation: Porto (Portugal)
  • Year and Date: 2014-06-20
  • Invited
• [Presentation] Exploring the catabolism of glycans in Saccharomyces cerevisiae. (2013)
  • Author(s): Tadashi Suzuki
  • Organizer: 2013 International Glycoforum
  • Place of Presentation: 江南大学（無錫、 中国）
  • Invited
• [Presentation] Yeast oligosaccharyltransferase generates free oligosaccharides during N-glycosylation. (2013)
  • Author(s): Yoichiro Harara, and Tadashi Suzuki
  • Organizer: RIKEN-Max Planck Joint Research Center for Systems Chemical Biology The Second Symposium
  • Place of Presentation: 理化学研究所（和光）
• [Presentation] Discovery of the novel type of free glycans in Saccharomyces cerevisiae. (2013)
  • Author(s): Hiroto Hirayama and Tadashi Suzuki
  • Organizer: RIKEN-Max Planck Joint Research Center for Systems Chemical Biology The Second Symposium
  • Place of Presentation: 理化学研究所（和光）
• [Presentation] Discovery of the novel type of free glycans in Saccharomyces cerevisiae. (2013)
  • Author(s): Hiroto Hirayama and Tadashi Suzuki
  • Organizer: The 3rd Austria/Japan Seminar on Comparative and Developmental Glycobiology
  • Place of Presentation: 理化学研究所（和光）
• [Presentation] 出芽酵母のO-結合型糖鎖様構造を持つ遊離糖鎖とその機能について (2013)
  • Author(s): 平山弘人、鈴木 匡
  • Organizer: 第32回日本糖質学会年会
  • Place of Presentation: 大阪国際交流センター（大阪）
• [Presentation] ドリコールオリゴ糖前駆体の品質管理機構 (2013)
  • Author(s): 原田陽一郎、中嶋和紀、正原-根岸由紀、安形高志、谷口直之、鈴木 匡
  • Organizer: 第32回日本糖質学会年会
  • Place of Presentation: 大阪国際交流センター（大阪）
• [Presentation] 出芽酵母の遊離糖鎖生成とその調節について (2013)
  • Author(s): 平山 弘人、鈴木 匡
  • Organizer: 第46回酵母遺伝学フォーラム
  • Place of Presentation: 東北学院大学（仙台）
• [Presentation] ドリコールオリゴ糖の品質管理機構の同定 (2013)
  • Author(s): 原田陽一郎、中嶋和紀、正原-根岸由紀、安形高志、谷口直之、鈴木 匡
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜（横浜）
• [Presentation] 出芽酵母の生成する遊離糖鎖とその機能について (2013)
  • Author(s): 平山弘人、鈴木 匡
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜（横浜）
  • Invited
• [Presentation] 出芽酵母における糖鎖の代謝機構-我々は何を知っているのか？？
  • Author(s): 鈴木 匡
  • Organizer: Interdisciplinary Symposium on Advanced Biology and Biotechnology 2013
  • Place of Presentation: 産業科学総合研究所（つくば）
  • Invited
• [Book] N-glycans and quality control of proteins. in Sugar Chains (T. Suzuki, K. Ohtsubo and N. Taniguchi eds.) (2015)
  • Author(s): Nobuko Hosokawa and Tadashi Suzuki
  • Publisher: springer
• [Book] OST complex (OST48, Ribophorin I, Ribophorin II, DAD1) in “Handbook of glycosyltransferases and related genes. 2nd Edition” (N. Taniguchi, K. Honke, M. Fukuda, H. Narimatsu, Y. Yamaguchi, T. Angata eds.) (2014)
  • Author(s): Yoichiro Harada and Tadashi Suzuki
  • Publisher: Springer
• [Book] GDP-mannose pyrophophorylase A, B (GMPPA,B) in Handbook of glycosyltransferases and related genes (N. Taniguchi, K. Honke, M. Fukuda, H. Narimatsu, Y. Yamaguchi, T. Angata eds.). (2014)
  • Author(s): Hiroto Hirayama and Tadashi Suzuki
  • Publisher: Springer
• [Book] Overview of the quality control system for glycoproteins in the endoplasmic reticulum. in Glycoscience: Biology and Medicine. (N. Taniguchi, T. Endo, G. W. Hart, P. Seeberger, C-H. Wong eds.) (2014)
  • Author(s): Tadashi Suzuki
  • Publisher: Springer
• [Book] Free oligosaccharides (high mannose type free glycans found in mammals and yeast) in Glycoscience: Biology and Medicine. (N. Taniguchi, T. Endo, G. W. Hart, P. Seeberger, C-H. Wong eds.) (2014)
  • Author(s): Tadashi Suzuki
  • Publisher: Springer
• [Book] OST complex (OST48, Ribophorin I, Ribophorin II, DAD1) (Vol.3, 1255-1272). In "Handbook of glycosyltransferases and related genes. 2nd Edition" (N. Taniguchi, K. Honke, M. Fukuda, H. Narimatsu, Y. Yamaguchi, T. Angata eds.) (2014)
  • Author(s): Yoichiro Harada and Tadashi Suzuki
  • Total Pages: 1707
  • Publisher: Springer
• [Book] GDP-mannose pyrophophorylase A, B (GMPPA,B) (Vol. 3, 1599-1606). In "Handbook of glycosyltransferases and related genes. 2nd Edition" (N. Taniguchi, K. Honke, M. Fukuda, H. Narimatsu, Y. Yamaguchi, T. Angata eds.) (2014)
  • Author(s): Hiroto Hirayama and Tadashi Suzuki
  • Total Pages: 1707
  • Publisher: Springer
• [Book] Free oligosaccharides: formation and degradation (Vol. 2, pp. 329-331). In “Encyclopedia of Biological Chemistry 2nd Editon” (W. J. Lennarz and M. D. Lane, eds.) (2013)
  • Author(s): Tadashi Suzuki
  • Total Pages: 3232
  • Publisher: Academic Press
• [Book] N-linked glycan processing glucosidases and mannosidases (Vol. 3, pp. 257-260). In “Encyclopedia of Biological Chemistry 2nd Editon” (W. J. Lennarz and M. D. Lane, eds.) (2013)
  • Author(s): Tadashi Suzuki
  • Total Pages: 3232
  • Publisher: Academic Press
• [Remarks] 不完全な糖鎖をきれいに分解するメカニズムを発見
  • URL: http://www.riken.jp/pr/press/2013/20131112_1/
• [Remarks] オリゴ糖転移酵素（OST）は糖鎖修飾中に遊離糖鎖を生成する
  • URL: http://www.riken.jp/pr/press/2013/20130924_2/
• [Remarks] In search of a sugar’s secrets
  • URL: http://www.riken.jp/en/research/rikenresearch/highlights/7539/