• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Mechanical Regulation of cytoplasmic dynein motility

Research Project

Project/Area Number 25291031
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Biophysics
Research InstitutionThe University of Tokyo

Principal Investigator

TOYOSHIMA Yoko  東京大学, 総合文化研究科, 教授 (40158043)

Co-Investigator(Kenkyū-buntansha) YAJIMA Junichiro  東京大学, 大学院総合文化研究科, 准教授 (00453499)
Research Collaborator KOBAYASHI Takuya  
SUGAWA Mitsuhiro  
TORISAWA Takayuki  
ICHIKAWA Munenori  
SAITO Kei  
KAJITA Hatsuha  
MIYASHITA Takuya  
SHIBATA Keitaro  
UTSUMI Yui  
FURUTA Ken'ya  
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2013: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Keywords細胞質ダイニン / 微小管 / モーター活性 / 頭部間距離 / メカニカル制御 / ブラウン運動 / 方向性の運動 / キネシン / ダイナクチン / ダイニン / 自己制御機構 / 拡散運動 / 一方向運動 / スタック構造 / 1分子運動観察 / 電子顕微鏡観察 / ラチェット機構 / キャッチ機構
Outline of Final Research Achievements

To investigate the molecular mechanism of dynein motility, we have genetically engineered human dynein heavy chain and observed single molecule behaviors of the recombinant proteins on the microtubule and their molecular shape by electron microscopy. When dynein didn’t have the cargo, namely under no load, the ring structure of the two heads overlapped in face-to-face fashion and the dynein molecules fluctuated on the microtubule without showing unidirectional movement, falling in self-inhibited state. When the two heads were restricted not to overlapped, the molecules exhibited the unidirectional and processive movement toward the microtubule minus end under no load.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (32 results)

All 2015 2014 2013

All Journal Article (10 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 10 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (22 results) (of which Invited: 4 results)

  • [Journal Article] Axonemal dynein light chain-1 locates at the microtubule-binding domain of the γ heavy chain.2015

    • Author(s)
      Ichikawa M, Saito K, Yanagisawa HA, Yagi T, Kamiya R, Yamaguchi S, Yajima J, Kushida Y, Nakano K, Numata O and Toyoshima YY.
    • Journal Title

      Molicular Biology of the Cell

      Volume: 26 Issue: 23 Pages: 4236-47

    • DOI

      10.1091/mbc.e15-05-0289

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] α- and β-tubulin lattice arrangement of the axonemal microtubule doublet and binding proteins revealed by single particle cryo-electron microscopy and tomography.2015

    • Author(s)
      Maheshwari, A., Obbineni, J. M., Bui, K. H., Shibata, K., Toyoshima Y. Y. & Ishikawa, T.
    • Journal Title

      Structure

      Volume: 23 Issue: 9 Pages: 1584-1595

    • DOI

      10.1016/j.str.2015.06.017

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Regulatory Mechanism of Cytoplasmic Dynein2015

    • Author(s)
      鳥澤嵩征、古田健也、豊島陽子
    • Journal Title

      Seibutsu Butsuri

      Volume: 55 Issue: 3 Pages: 127-132

    • DOI

      10.2142/biophys.55.127

    • NAID

      130005072019

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Autoinhibition and cooperative activation mechanisms of cytoplasmic dynein2014

    • Author(s)
      Torisawa, T., Ichikawa, M., Furuta, A., Saito, K., Oiwa, K., Kojima, H., Toyoshima, Y. Y. and Furuta, K.
    • Journal Title

      Nature Cell Biol

      Volume: 16 Issue: 11 Pages: 1118-1118

    • DOI

      10.1038/ncb3048

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Structure of the entire stalk region of the dynein motor domain2014

    • Author(s)
      Yosuke Nishikawa, Takuji Oyama, Narutoshi Kamiya, Takahide Kon, Yoko Y. Toyoshima, Haruki Nakamura, Genji Kurisu
    • Journal Title

      Journal of Molecular Biology

      Volume: 426 Issue: 19 Pages: 3232-3245

    • DOI

      10.1016/j.jmb.2014.06.023

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] バイオイメージングと光ピンセットを用いた微小管系モータータンパク質の協働的活性化に関する働き2014

    • Author(s)
      古田健也、鳥澤嵩征、豊島陽子
    • Journal Title

      生化学

      Volume: 86 Pages: 184-191

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Formation of ring-shaped microtubule assemblies through active self-organization on dynein.2014

    • Author(s)
      Ito, M., Arif Md. Rashedul Kabir, A.,M.,R. Inoue, D., Torisawa, T, Toyoshima, Y.Y., Sada1, K. & Kakugo
    • Journal Title

      Polymer Jouranl

      Volume: 46 Pages: 220-225

    • NAID

      120005515308

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sea lily muscle lacks a troponin-regulatory system but contains paramyosin.2014

    • Author(s)
      Obinata T, Amemiya S, Takai R, Ichikawa M, Toyosima YY, Sato N
    • Journal Title

      Zoological Science

      Volume: 31 Issue: 3 Pages: 122-128

    • DOI

      10.2108/zsj.31.122

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Measuring Collective Transport by Defined Numbers of Processive and Nonprocessive Kinesin Motors2013

    • Author(s)
      古田 健也, 古田 茜, 豊島 陽子, 網野 美紗子, 大岩 和弘, 小嶋 寛明
    • Journal Title

      Proc Natl Acad Sci U S A ( 米国科学アカデミー紀要)

      Volume: 110(2) Issue: 2 Pages: 501-6

    • DOI

      10.1073/pnas.1201390110

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Formation of Ring-Shaped Microtubule Assemblies through Active Self-Organization on Dynein2013

    • Author(s)
      Masaki Ito, Arif Md Rashedul Kabir, Daisuke Inoue, Takayuki Torisawa, Yoko Toyoshima, Kazuki Sada, Akira Kakugo
    • Journal Title

      Polymer Journal

      Volume: 46 Issue: 4 Pages: 220-225

    • DOI

      10.1038/pj.2013.89

    • NAID

      120005515308

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] Axonemal Dynein Light Chain-1 Locates at the Microtubule Binding Domain of the γ Heavy Chain2015

    • Author(s)
      Ichikawa M., Saito K., Yanagisawa H., Yagi, T., Kamiya, R., Yamaguchi S., Yajima, J., Kushida Y., Nakano, K., Numata, O. and Toyoshima, Y. Y.
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県・金沢市)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] 分裂期kinesin-6の運動特性に関する研究2015

    • Author(s)
      Yohei Maruyama, Akihiko Sato, Tim Davis, Toshihisa Osaki, Shin Yamaguchi, Shoji Takeuchi, Masanori Mishima, Junichiro Yajima
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県・金沢市)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] Off-axis motion of yeast cytoplasmic dynein takes a biased random walk2015

    • Author(s)
      Sugawa, M., Yamaguchi, S., Takagi, H., Shibata, K., Toyoshima, Y. Y., Yajima, J
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県・金沢市)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] Dynactin is a biphasic regulator to promote and inhibit dynein motility2015

    • Author(s)
      Kobayashi, K., Miyashita, K., Kajita, H., Saito, K., Murayama, T., Toyoshima, Y. Y.
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県・金沢市)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] 如何にしてキネシンは運動方向を逆にするのか2015

    • Author(s)
      山岸雅彦、矢島潤一郎
    • Organizer
      第5回分子モーター討論会
    • Place of Presentation
      東京大学(東京・目黒区)
    • Year and Date
      2015-06-13
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Cytoplasmic dynein takes a route switching randomly between protofilaments with a bias toward the right2014

    • Author(s)
      Mitsuhiro Sugawa, Shin Yamaguchi, Keitaro Shibata, Yoko Y. Toyoshima, Junichiro Yajima
    • Organizer
      第52回日本生物物理学会年会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2014-09-13 – 2014-09-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] Autoinhibiton and cooperative activation mechanisms of cytoplasmic dynein.2014

    • Author(s)
      Takayuki Torisawa, Ken’ya Furuta, Muneyoshi Ichikawa, Akane Furuta, Kei Saito, Kazuhiro Oiwa, Hiroaki Kojima, Yoko Toyoshim
    • Organizer
      第52回日本生物物理学会年会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2014-09-13 – 2014-09-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] A novel role of dynactin for dynein motility revealed by gliding assay2014

    • Author(s)
      Takuya Kobayashi, Hatsuha Kajita, Kei Saito, Yoko Y. Toyoshima
    • Organizer
      第52回日本生物物理学会年会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2014-09-13 – 2014-09-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] A novel role of dynactin for dynein motility revealed by single-molecule assay2014

    • Author(s)
      Takuya Miyashita, Takuya Kobayashi, Hatsuha Kajita, Yoko Y. Toyoshima
    • Organizer
      第52回日本生物物理学会年会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2014-09-13 – 2014-09-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] New Folding Pattern of p150 and “antenna” for dynein binding2014

    • Author(s)
      Kei Saito, Takashi Murayama, Tomonori Hata, Takuya Kobayashi, Yoko Toyoshima
    • Organizer
      Biophysical Society 58th Annual Meeting
    • Place of Presentation
      San Francisco, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Self-regulation of cytoplasmic dynein through its unconventional force response.2014

    • Author(s)
      Takayuki Torisawa, Ken’ya Furuta, Akane Furuta, Muneyoshi Ichikawa, Kei Saito, Kazuhiro Oiwa, Hiroaki Kojima, Yoko Toyoshima
    • Organizer
      Biophysical Society 58th Annual Meeting
    • Place of Presentation
      San Francisco, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Torque generation of axonemal outer arm dyneins2014

    • Author(s)
      Shin Yamaguchi, Kei Saito, Miki Sutoh, Takayuki Nishizaka, Yoko Y Toyoshima, Junichiro Yajima,
    • Organizer
      Biophysical Society 58th Annual Meeting
    • Place of Presentation
      San Francisco, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Autoinhibition and synergistid activation of cytoplasmic dynein2013

    • Author(s)
      Takayuki Torisawa, Ken’ya Furuta, Akane Furuta, Muneyoshi Ichikawa, Yoko Toyoshima
    • Organizer
      日本生物物理学会第51回年会
    • Place of Presentation
      京都府、京都国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] Regulatory mechanism of mictrotubule –based molecular motors by Bicaudal-D22013

    • Author(s)
      Takuya Kobayashi, Akira Hanashima, Yoko Toyoshima, Takashi Murayama
    • Organizer
      日本生物物理学会第51回年会
    • Place of Presentation
      京都府、京都国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] Molecular architecture of dynactin p1502013

    • Author(s)
      Kei Saito, Takashi Murayama, Tomonori Hata, Yoko Toyoshima
    • Organizer
      日本生物物理学会第51回年会
    • Place of Presentation
      京都府、京都国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] Subunit structure of Tetrahymena outer dynein arm complex2013

    • Author(s)
      Muneyoshi Ichikawa, Yasuharu Kushida, Kentaro Nakano, Osamu Numata, Yoko Toyoshima
    • Organizer
      日本生物物理学会第51回年会
    • Place of Presentation
      京都府、京都国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] The mechanism of determining the directionality of Ncd2013

    • Author(s)
      Masahiko Yamagishi, Yoko Toyoshima, Junichiro Yajima
    • Organizer
      日本生物物理学会第51回年会
    • Place of Presentation
      京都府、京都国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] Submolecular structure of cytoplasmic dynein tail and dynactin sidearm.2013

    • Author(s)
      Kei Saito, Yuta Watahabe, Muneyoshi Ichikawa, Tomonori Hata, Takuya Kobayashi, Takashi Murayama, Yoko Toyoshima
    • Organizer
      International Workshop Dynein2013
    • Place of Presentation
      兵庫県、神戸オルビスホール
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Self-regulation of cytoplasmic dynein2013

    • Author(s)
      Ken’ya Furuta, Takayuyki Torisawa, Akane Furuta, Muneyoshi Ichikawa, Kei Saito, Kazuhiro Oiwa, Hiroaki Kojima, Yoko Toyoshima
    • Organizer
      International Workshop Dynein2013
    • Place of Presentation
      兵庫県、神戸オルビスホール
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Torque generation by axonemal outer-arm dynein2013

    • Author(s)
      Shin Yamaguchi, Yoko Toyoshima, Junichiro Yajima
    • Organizer
      International Workshop Dynein2013
    • Place of Presentation
      兵庫県、神戸オルビスホール
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Self-regulation of cytoplasmic dynein2013

    • Author(s)
      Ken'ya Furuta, Takayuki Torisawa, Akane Furuta, Muneyoshi Ichikawa, Kei Saito, Kauzuhiro Oiwa, Hiroaki Kojima, Yoko Toyoshima
    • Organizer
      American Society for Cell Biology 2013 Annual Meeting
    • Place of Presentation
      New Orleans, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Submolecular structure of dynactin sidearm: a new folding pattern of p1502013

    • Author(s)
      Kei Saito, Tomonori Hata, Takuya Kobayashi, Takashi Murayama, Yoko Toyoshima
    • Organizer
      American Society for Cell Biology 2013 Annual Meeting
    • Place of Presentation
      New Orleans, USA
    • Related Report
      2013 Annual Research Report

URL: 

Published: 2013-05-21   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi