Nanoquantification of the regulatory mechanism of molecular dynamics by development of super-resolution microscopy using single molecule imaging
| Project/Area Number |
25291032
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
Tokunaga Makio 東京工業大学, 生命理工学研究科, 教授 (00192659)
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| Co-Investigator(Renkei-kenkyūsha) |
SOGAWA KUMIKO (SAKATA KUMIKO) 東京工業大学, 生命理工学研究科, 准教授 (20291073)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Keywords | １分子イメージング / バイオイメージング / 細胞機能と構造 / 超解像顕微鏡 / 生体分子計測 / 生細胞分子動態 / 生物物理 / 細胞情報・動態 |
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| Outline of Final Research Achievements |
We have developed an integrated method for quantitative analysis of molecular dynamics and interactions, combining multiple methods of microscopy, multi-color single-molecule imaging, single molecule tracking and super-resolution microscopy. We have improved resolution in multi-color single-molecule microscopy by optimization of illumination and stabilization of optics. We also imporved accuracy in analysis method for superesolution single-molecule microscopy by optimizing alignment accuracy. The improved integrated microscopy provides a means to obtain new view of spatio-temporal and kinetic parameters of molecular dynamics and interactions in living cells. We carried out imaging analysis of molecules involved in signal transduction and translation.
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Report
(4 results)
Research Products
(46 results)
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[Journal Article] Regulation of RNA polymerase II activation by histone acetylation in single living cells2014
Author(s)
Stasevich TJ, Hayashi-Takanaka Y, Sato Y, Maehara K, Ohkawa Y, Sakata-Sogawa K, Tokunaga M, Nagase T, Nozaki N, McNally JG, Kimura H
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Journal Title
Nature
Volume: 516
Issue: 7530
Pages: 272-275
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] 細胞分化における核内アクチンの機能解析2016
Author(s)
山本 浩志, 山崎 祥他, 十川 久美子, 徳永 万喜洋, 原田 昌彦
Organizer
第33回染色体ワークショップ・第14回核ダイナミクス研究会
Place of Presentation
一の坊(宮城県松島町)
Year and Date
2016-01-12
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[Presentation] Roles of nuclear filamentous-actin in transcriptional regulation2014
Author(s)
Shota Yamazaki, Koji Yamamoto, Kumiko Sakata-Sogawa, Makio Tokunaga, Masahiko Harata
Organizer
2014 ASCB/IFCB Meeting (The American Society for Cell Biology / International Federation for Cell Biology)
Place of Presentation
Pennsylvania Convention Center, Philadelphia, USA
Year and Date
2014-12-06 – 2014-12-10
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