| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Outline of Final Research Achievements |
We have developed an integrated method for quantitative analysis of molecular dynamics and interactions, combining multiple methods of microscopy, multi-color single-molecule imaging, single molecule tracking and super-resolution microscopy. We have improved resolution in multi-color single-molecule microscopy by optimization of illumination and stabilization of optics. We also imporved accuracy in analysis method for superesolution single-molecule microscopy by optimizing alignment accuracy. The improved integrated microscopy provides a means to obtain new view of spatio-temporal and kinetic parameters of molecular dynamics and interactions in living cells. We carried out imaging analysis of molecules involved in signal transduction and translation.
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