New Paradigm for Theory of Protein Hydration: Elucidation of Biological Self-Assembly Processes in a Unified Manner
Project/Area Number |
25291035
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Biophysics
|
Research Institution | Kyoto University |
Principal Investigator |
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2015: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
|
Keywords | 自己組織化 / 構造形成 / フォールディング / 分子認識 / ATP駆動タンパク質 / 水和 / エントロピー / 積分方程式論 / ATP駆動蛋白質 / 秩序構造形成 / 水 / 蛋白質 / 生体分子 / 統計熱力学 |
Outline of Final Research Achievements |
The structures formed by the self-assembly of biomolecules are collapsed by applying high pressures (e.g., pressure denaturation of a protein, dissociation of filamentous actin (F-actin) into monomers, and destruction of amyloid fibril). The power of the formation becomes weaker at low temperatures (e.g., cold denaturation of a protein and weakened binding of myosin to F-actin). We have shown the following: These experimental facts are governed by the biomolecule-water many-body correlation component of the effect of configurational entropy of water; protein folding, various types of denaturation, and cosolvent effects on protein stability can be elucidated in a unified manner within the same theoretical framework; the diverse molecular recognitions share the same mechanism; physics of the multidrug efflux by ABC transporter is explainable; and a novel concept can be conferred upon the mechanism of functional expression of ATP-driven proteins.
|
Report
(4 results)
Research Products
(81 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Expression, purification and crystallization of 5-HT2A receptor2015
Author(s)
N. Suzuki, A. Saito, K. Hitomi, Y. Takamuku, K. Mizutani, Y. Kajiwara, S. Yasuda, M. Kinoshita, and T. Murata
Organizer
GPCR workshop 2015
Place of Presentation
Big island, Hawaii (USA)
Year and Date
2015-12-01
Related Report
-
-
-
[Presentation] Towards structure determination of the human prostanoid receptor bound to the antibody2015
Author(s)
Y. Toyoda, K. Morimoto, R. Suno, Y. Sekiguchi, K. Yamashita, K. Hirata, S. Yasuda, Y. Kajiwara, T. Shimizu, Y. Urushibata, S. Yoshida, T. Kuribara, T. Hosoya, M. Kinoshita, Y. Sugimoto, N. Nomura, T. Murata, K. Takayama, M. Yamamoto, S. Narumiya, S. Iwata, and T. Kobayashi
Organizer
GPCR workshop 2015
Place of Presentation
Big island, Hawaii (USA)
Year and Date
2015-12-01
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-