| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2015: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
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| Outline of Final Research Achievements |
Solution X-ray and neutron scattering analyses have showed that both ends of double stranded DNA in H2A.B nucleosome are peeled off from the histone octamer and is exposed to the solvent. This facilitates the chromatin takes a looser conformation than H2A nucleosome. Also, solution X-ray scattering analysis indicates that conformation of higher-order nucleosome complex, H3-(CENP-A)-H3 tri-nucleosome is more opened than that of H3-H3-H3 tri-nucleosome. Furthermore, MD-SAXS analysis (solution X-ray scattering analysis combined with molecular dynamics calculation) using a coarse grained model as a tri-nucleosome has analyzed the dynamical structure of the chromatin in centromere and non-centromere regions at atomic resolution and elucidated the functional significance of the chromatin containing CENP-A nucleosome in the centromere.
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