Structural chemistry of O2-tolerant [NiFe]-hydrogenases

• Project/Area Number: 25291038
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biophysics
• Research Institution: University of Hyogo
• Principal Investigator: Higuchi Yoshiki 兵庫県立大学, 生命理学研究科, 教授 (90183574)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000); Fiscal Year 2015: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000); Fiscal Year 2014: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000); Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: X線結晶解析 / 酸素耐性酵素 / Ni酵素 / ヒドロゲナーゼ / 燃料電池 / 中性子結晶解析 / 反応機構 / プロトンチャネル / 中性子解析

Outline of Final Research Achievements

In this study, x-ray structure analysis of newly found O2-tolerant [NiFe]-hydrogenase and neutron structure analysis of standard enzyme have been carried out.
1. The x-ray structures of the newly found O2-tolerant [NiFe]-hydrogenase from Citrobacter sp. S-77 in the air-oxidized, K3Fe(CN)6-oxidized and H2-reduced forms have been successfully solved at the resolutions better than 2.0 A. The one of the iron-sulfur clusters (the proximal to the Ni-Fe active site) is not a [4Fe3S]-type, which was found in the usual O2-tolerant enzymes, but a [4Fe4S]-type typical as those in the standard enzymes. This [4Fe4S]-type cluster changed its structure when the enzyme is oxidized by K3Fe(CN)6, but the mechanism of the structural changed are different from those clusters in O2-tolerant hydrogenase.
2. Neutron diffraction data up to 2.0 A resolution have been successfully obtained from a large crystal (>1.5 mm3) grown in D2O.

Research Products

• [Int'l Joint Research] マックスプランク研究所(ドイツ)
• [Journal Article] Domain swapping oligomerization of thermostable c-type cytochrome in E. coli cells (2016)
  • Author(s): Yugo Hayashi, Masaru Yamanaka, Satoshi Nagao, Hirofumi Komori, Yoshiki Higuchi, Shun Hirota
  • Journal Title: Scientific reports Volume: 6 Issue: 1 Pages: 19334-19334
  • DOI: 10.1038/srep19334
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Synthesis and Reactivity of a Water-soluble NiRu Monohydride Complex with a Tethered Pyridine Moiety (2016)
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  • NAID: 130005124717
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• [Journal Article] Improved purification, crystallization and crystallographic study of Hyd-2 type [NiFe] hydrogenase from Citrobacter sp. S-77 (2016)
  • Author(s): Muhd Noor, N.D.; Nishikawa, Koji; Nishihara, Hirofumi; Yoon, Ki-Seok; Ogo, Seiji; Higuchi, Yoshiki
  • Journal Title: Acta Crystallogr. Sect. F Struct. Biol. Commun. Volume: F72 Issue: 1 Pages: 53-58
  • DOI: 10.1107/s2053230x15024152
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• [Journal Article] Bioelectrochemical analysis of thermodynamics of the catalytic cycle and kinetics of the oxidative inactivation of oxygen-tolerant [NiFe]-hydrogenase (2016)
  • Author(s): Keisei So, Rui Hamamoto, Ryosuke Takeuchi, Yuki Kitazumi, Osamu Shirai, Ryohei Endo, Hirofumi Nishihara, Yoshiki Higuchi, Kenji Kano
  • Journal Title: Journal of Electroanalytical Chemistry Volume: 766 Pages: 152-161
  • DOI: 10.1016/j.jelechem.2016.02.009
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] ヒドロゲナーゼによる水素分解・合成機構および酵素燃料電池への応用 (2016)
  • Author(s): 廣田俊，加納健司，樋口芳樹
  • Journal Title: BIO INDUSTRY Volume: 33(1) Pages: 43-48
  • Acknowledgement Compliant
• [Journal Article] Domain-swapped dimer of Pseudomonas aeruginosa cytochrome c551: Structural insights into domain swapping of cytochrome c family proteins (2015)
  • Author(s): Satoshi Nagao, Mariko Ueda, Hisao Osuka, Hirofumi Komori, Hironari Kamikubo, Mikio Kataoka, Yoshiki Higuchi, Shun Hirota
  • Journal Title: PLoS One Volume: 10 Issue: 4 Pages: e0123653-e0123653
  • DOI: 10.1371/journal.pone.0123653
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• [Journal Article] Structural basis for cargo binding and autoinhibition of Bicaudal-D1 by a parallel coiled-coil with homotypic registry. (2015)
  • Author(s): Terawaki S, Yoshikane A, Higuchi Y, Wakamatsu K.
  • Journal Title: Biochem Biophys Res Commun. Volume: 460 Issue: 2 Pages: 451-456
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  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] FT-IR Characterization of the Light-Induced Ni-L2 and Ni-L3 States of [NiFe] Hydrogenase from Desulfovibrio vulgaris Miyazaki F (2015)
  • Author(s): H. Tai, K. Nishikawa, S. Inoue, Y. Higuchi and S. Hirota
  • Journal Title: J. Phys. Chem. B Volume: 119 Issue: 43 Pages: 13668-13674
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• [Journal Article] Crystallization and Preliminary X-ray Diffraction Analysis of FAD-dependent Glucose Dehydrogenase (2015)
  • Author(s): H. Komori, K. Inaka, N. Furubayashi, M. Honda and Y. Higuchi
  • Journal Title: Acta Crystallogr. F Volume: 71 Issue: 8 Pages: 1017-1019
  • DOI: 10.1107/s2053230x15010742
  • Peer Reviewed
• [Journal Article] “Newton’s cradle” proton relay with amide-imidic acid tautomerization in inverting cellulase visualized by neutron crystallography (2015)
  • Author(s): Nakamura, A., Ishida, T., Kusaka, K., Yamada, T., Fushinobu, S., Tanaka, I., Kaneko, S., Ohta, K., Tanaka, H., Inaka, K., Higuchi, Y., Niimura, N., Samejima, M., and Igarashi, K.
  • Journal Title: Science Adv. Volume: 1 Issue: 7
  • DOI: 10.1126/sciadv.1500263
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• [Journal Article] Nanoscale charge transport in cytochrome c3/DNA network: Comparative studies between redox-active molecules (2015)
  • Author(s): H. Yamaguchi, D.-C. Che, Y. Hirano, M. Suzuki, Y. Higuchi and T. Matsumoto
  • Journal Title: Japanese Journal of Applied Physics Volume: 54 Issue: 9 Pages: 095201-095204
  • DOI: 10.7567/jjap.54.095201
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• [Journal Article] Structural Insights into the O2 Reduction Mechanism of Multicopper Oxidase (2015)
  • Author(s): H. Komori and Y. Higuchi
  • Journal Title: J. Biochem. (Tokyo) Volume: 158(4) Issue: 4 Pages: 293-298
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• [Journal Article] Oligomerization Enhancement and Two Domain Swapping Mode Detection for Thermostable Cytochrome c-552 via the Elongation of the Major Hinge Loop (2015)
  • Author(s): C. Ren, S. Nagao, M. Yamanaka, H. Komori, Y. Shomura, Y. Higuchi and S. Hirota
  • Journal Title: Mol. Biosyst. Volume: 11(12) Issue: 12 Pages: 218-221
  • DOI: 10.1039/c5mb00545k
  • NAID: 120006334941
  • Peer Reviewed
• [Journal Article] Domain-swapped Cytochrome cb562 Dimer and Its Nanocage Encapsulating a Zn-SO4 Cluster in the Internal Cavity (2015)
  • Author(s): Takaaki Miyamoto, Mai Kuribayashi, Satoshi Nagao, Yasuhito Shomura, Yoshiki Higuchi, Shun Hirota
  • Journal Title: Chemical Science Volume: 6 Issue: 12 Pages: 7336-7342
  • DOI: 10.1039/c5sc02428e
  • NAID: 120006334940
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Rational design of heterodimeric protein using domain swapping for myoglobin (2015)
  • Author(s): Ying-Wu Lin, Satoshi Nagao, Mohan Zhang, Yasuhito Shomura, Yoshiki Higuchi, Shun Hirota
  • Journal Title: Angewandte Chemie International Edition Volume: 54 Issue: 2 Pages: 511-515
  • DOI: 10.1002/anie.201409267
  • NAID: 120005716859
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Crystallization and preliminary X-ray analysis of the NAD+-reducing [NiFe] hydrogenase from Hydrogenophilus thermoluteolus TH-1. (2015)
  • Author(s): Taketa M, Nakagawa H, Habukawa M, Osuka H, Kihira K, Komori H, Shibata N, Ishii M, Igarashi Y, Nishihara H, Yoon KS, Ogo S, Shomura Y, Higuchi Y.
  • Journal Title: Acta Crystallogr F Struct Biol Commun. Volume: 71 Issue: 1 Pages: 96-99
  • DOI: 10.1107/s2053230x14026521
  • Peer Reviewed
• [Journal Article] Light-Driven Hydrogen Production by Hydrogenases and a Ru- Complex inside a Nanoporous Glass Plate under Aerobic External Conditions (2014)
  • Author(s): Tomoyasu Noji, Masaharu Kondo, Tetsuro Jin, Tetsuo Yazawa, Hisao Osuka, Yoshiki Higuchi, Mamoru Nango, Shigeru Itoh, Takehisa Dewa
  • Journal Title: Journal of Physical Chemistry Letters Volume: 5 Issue: 14 Pages: 2403-2407
  • DOI: 10.1021/jz5008164
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Crystallographic characterization of the C-terminal coiled-coil region of mouse Bicaudal-D1 (BICD1) (2014)
  • Author(s): S. Terawaki, H. Ootsuka, Y. Higuchi, and K. Wakamatsu
  • Journal Title: Acta Cryst.F Volume: 70 Issue: 8 Pages: 1103-1106
  • DOI: 10.1107/s2053230x1401276x
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Formation of Domain-Swapped Oligomer of Cytochrome c from Its Molten Globule State Oligomer (2014)
  • Author(s): Megha Deshpande, Partha Parui, Hironari Kamikubo, Masaru Yamanaka, Satoshi Nagao, Hirofumi Komori, Mikio Kataoka, Yoshiki Higuchi, Shun Hirota
  • Journal Title: Biochemistry Volume: 53(28) Issue: 28 Pages: 4696-4703
  • DOI: 10.1021/bi500497s
  • Peer Reviewed
• [Journal Article] Gas-diffusion and Direct Electron Transfer-type Bioanode for Hydrogen Oxidation with Oxygen-tolerant [NiFe]-hydrogenase as an Electrocatalyst (2014)
  • Author(s): K. So, Y. Kitazumi, O. Shirai, K. Kurita, H. Nishihara, Y. Higuchi, K. Kano
  • Journal Title: Chemistry Lett. Volume: 43 Pages: 1575-1577
  • NAID: 130004427222
  • Peer Reviewed
• [Journal Article] Catalytic Cycle of Ctochrome-c3 Hydrogenase, a [NiFe]-Enzyme, Deduced from the Structures of the Enzyme and the Enzyme Mimic (2014)
  • Author(s): Yagi, Tatsuhiko; Ogo, Seiji; Higuchi, Yoshiki
  • Journal Title: Int. J. Hydrogen Energy Volume: 印刷中 Issue: 32 Pages: 18543-18550
  • DOI: 10.1016/j.ijhydene.2013.12.174
  • Peer Reviewed
• [Journal Article] Kinetic Analysis of Inactivation and Enzyme Reaction of Oxygen-tolerant [NiFe]-hydrogenase at Direct Electron Transfer-type Bioanode (2014)
  • Author(s): K. So, Y. Kitazumi, O. Shirai, K. Kurita, H. Nishihara, Y. Higuchi, and K. Kano
  • Journal Title: Bull. Chem. Soc. Jpn. Volume: 87 Issue: 11 Pages: 1177-1185
  • DOI: 10.1246/bcsj.20140223
  • NAID: 130004684803
  • Peer Reviewed
• [Journal Article] Control of Transition between Ni-C and Ni-SIa States in Catalytic Cycle of [NiFe] Hydrogenase by Redox State of its Proximal Fe-S Cluster (2014)
  • Author(s): H. Tai, K. Nishikawa, M. Suzuki, Y. Higuchi, and S. Hirota
  • Journal Title: Angew. Chem. Int. Ed. Volume: 53 Issue: 50 Pages: 13817-13820
  • DOI: 10.1002/anie.201408552
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Change in Structure and Ligand Binding Properties of Hyperstable Cytochrome c555 from Aquifex aeolicus by Domain Swapping (2014)
  • Author(s): M. Yamanaka, S. Nagao, H. Komori, Y. Higuchi and S. Hirota
  • Journal Title: Protein Science Volume: 24 Issue: 3 Pages: 366-375
  • DOI: 10.1002/pro.2627
  • NAID: 120005716876
  • Peer Reviewed
• [Journal Article] New insights into the catalytic active-site structure of multicopper oxidases. (2014)
  • Author(s): Komori H, Sugiyama R, Kataoka K, Miyazaki K, Higuchi Y, Sakurai T
  • Journal Title: Acta Crystallogr D Biol Crystallogr Volume: 70 Issue: 3 Pages: 772-779
  • DOI: 10.1107/s1399004713033051
  • NAID: 120005451980
  • Peer Reviewed
• [Journal Article] Inhibitory activity of Filipendula ulmaria on recombinant histidine decarboxykase. (2013)
  • Author(s): Nitta Yoko
  • Journal Title: Food Chemistry Volume: 138 Issue: 2-3 Pages: 1551-1556
  • DOI: 10.1016/j.foodchem.2012.10.074
  • Peer Reviewed
• [Journal Article] Photosensitivity of the Ni-A State of [NiFe] Hydrogenase from Desulfovibrio vulgaris Miyazaki F with Visible Light (2013)
  • Author(s): H. Osuka, Y. Shomura, H. Komori, N. Shibata, S. Nagao, Y. Higuchi, S. Hirota
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 430 Issue: 1 Pages: 284-288
  • DOI: 10.1016/j.bbrc.2012.10.136
  • NAID: 120005716864
  • Peer Reviewed
• [Journal Article] Studies on hydrogenase (2013)
  • Author(s): T. Yagi, Y. Higuchi
  • Journal Title: Proceedings of the Japan Academy, Series B Volume: 89 Issue: 1 Pages: 16-33
  • DOI: 10.2183/pjab.89.16
  • NAID: 130003365103
  • ISSN: 0386-2208, 1349-2896
  • Peer Reviewed
• [Journal Article] Cyrstal structure of the CueO mutants at Glu506, the key amino acid located in the proton transfer pathway for dioxygen reduction (2013)
  • Author(s): H. Komori, T. Kajikawa, K. Kataoka, Y. Higuchi & T. Sakurai
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 438 Issue: 4 Pages: 686-690
  • DOI: 10.1016/j.bbrc.2013.07.121
  • Peer Reviewed
• [Journal Article] Crystallization and X-ray diffraction analysis of nylon hydrolase (NylC) from Arthrobacter sp (2013)
  • Author(s): Nagai, K., Yasuhira, K., Tanaka, Y., Kato, D., Takeo, M., Higuchi, Y., Negoro, S., & Shibata N.
  • Journal Title: KI72 Acta Cryst. Volume: F69 Issue: 10 Pages: 1151-1154
  • DOI: 10.1107/s1744309113024263
  • Peer Reviewed
• [Journal Article] Structural Aspects of [NiFe]-hydrogenases (2013)
  • Author(s): Y. Shomura and Y. Higuchi
  • Journal Title: Rev. Inorg. Chem. Volume: 33 Issue: 4 Pages: 173-192
  • DOI: 10.1515/revic-2013-0005
  • Peer Reviewed
• [Journal Article] Formation of Oligomeric Cytochrome c during Folding by lntermolecular Hydrophobic Interaction between N-and C-Terminal α-Helices (2013)
  • Author(s): Partha Pratim Parui, Megha Subhash Deshpande, Satoshi Nagao, Hironari Kamikubo. Hirofumi Komori. Yoshiki Higuchi, Miklo Kataoka . Shun Hirota
  • Journal Title: Biochemistry Volume: 52巻 Issue: 48 Pages: 8732-8744
  • DOI: 10.1021/bi400986g
  • Peer Reviewed
• [Journal Article] 膜結合型ヒドロゲナーゼの酸素耐性機構の解明 (2013)
  • Author(s): 庄村康人，樋口芳樹
  • Journal Title: 生物物理 Volume: 53-2 Pages: 82-85
  • NAID: 10031151786
• [Journal Article] 立体構造を基盤としたナイロン加水分解酵素の開発と耐熱化機構 (2013)
  • Author(s): 根来誠司，永井圭介，大島祥平，飯田一希，加藤太一郎，武尾正弘，持地広造， 柴田直樹，樋口芳樹
  • Journal Title: 酵素工学ニュース Volume: 69 Pages: 12-18
• [Presentation] 中性子結晶解析法を用いて酵素における水素分解の反応機構解明にせまる (2016)
  • Author(s): 樋口芳樹
  • Organizer: 茨城大学大学院理工学研究科 ミニシンポジウム
  • Place of Presentation: 茨城大学水戸キャンパス ライブラリーホール（茨城県水戸市）
  • Year and Date: 2016-02-22
  • Invited
• [Presentation] ヒドロゲナーゼの総合化学 (2015)
  • Author(s): 樋口芳樹
  • Organizer: Cat-on-Cat symposium in Himeji 2015
  • Place of Presentation: 姫路・西はりま地場産業センター（兵庫県姫路市）
  • Year and Date: 2015-12-10
  • Invited
• [Presentation] ヒドロゲナーゼがもつ鉄硫黄クラスターの構造的多様性 (2015)
  • Author(s): 庄村康人，Noor Dina Binti Muhd Noor，西 幸志，樋口芳樹
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] Crystal structure analysis of the Fe-S protein maturation factor Cia1 (2015)
  • Author(s): 松本卓樹，樋口芳樹，庄村康人
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] ヒドロゲナーゼの修飾用電極の評価と反応活性の解析 (2015)
  • Author(s): 窪田慎太郎，Buithanh Tan， 山崎徹，矢澤哲夫，樋口芳樹
  • Organizer: 第61回ポーラログラフィーおよび電気化学分析化学討論会
  • Place of Presentation: イーグレ姫路（兵庫県姫路市）
  • Year and Date: 2015-11-24
• [Presentation] 水素酸化還元酵素・[NiFe]-ヒドロゲナーゼの総合的構造化学を目指して (2015)
  • Author(s): 樋口芳樹
  • Organizer: 東京大学放射光連携研究機構 10周年記念講演会
  • Place of Presentation: 東京大学本郷キャンパス小柴ホール（東京都文京区）
  • Year and Date: 2015-11-13
  • Invited
• [Presentation] EPR spectroscopic studies of highly O2-stable [NiFe]-hydrogenase from Citrobacter sp. S-77 (2015)
  • Author(s): Hiroaki Matsuura, Hulin Tai, Koji Nishikawa, Ki-Seok Yoon, Seiji Ogo, Shun Hirota, Yoshiki Higuchi
  • Organizer: University of Hyogo (UH)－EMTECH, Universiti Putra Malaysia (UPM) 2nd Joint Symposium
  • Place of Presentation: 兵庫県立先端科学技術支援センター(兵庫県上郡町)
  • Year and Date: 2015-10-22
  • Int'l Joint Research
• [Presentation] Structural chemistry of hydrogenase by diffraction method (2015)
  • Author(s): 樋口芳樹
  • Organizer: 錯体化学討論会
  • Place of Presentation: 奈良女子大学（奈良県奈良市）
  • Year and Date: 2015-09-21
  • Invited
• [Presentation] 電子伝導性多孔質ガラス電極の作製 (2015)
  • Author(s): ブイタンタン，橋野廉，嶺重温，窪田慎太郎，樋口芳樹，山崎徹，矢澤哲夫
  • Organizer: 公益社団法人日本セラミック協会第28回秋季シンポジウム
  • Place of Presentation: 富山大学五福キャンパス（富山県富山市）
  • Year and Date: 2015-09-16
• [Presentation] Preparation of the crystals of NAD+-reducing [NiFe] hydrogenase in the reduced form (2015)
  • Author(s): Hisashi Nakashima, Midori Taketa, Hanae Nakagawa, Mao Habukawa, Naoki Shibata, Yasuhito Shomura, Yoshiki Higuchi
  • Organizer: Student exchange program between College of Natural Sciences in Dong-A University and School of Science in University of Hyogo
  • Place of Presentation: 兵庫県立先端科学技術支援センター(兵庫県上郡町)
  • Year and Date: 2015-08-22
  • Int'l Joint Research
• [Presentation] Control of the Transition between Ni-C and Ni-SIa States in the Catalytic Cycle of [NiFe] Hydrogenase by the Redox State of the Proximal Fe-S Cluster (2015)
  • Author(s): Hulin Tai, Koji Nishikawa, Seiya Inoue, Masayuki Suzuki, Yoshiki Higuchi and Shun Hirota
  • Organizer: 17th International Conference on Biological Inorganic Chemistry
  • Place of Presentation: 北京，中国
  • Year and Date: 2015-07-20
  • Int'l Joint Research
• [Presentation] ヒドロゲナーゼの水素活性化反応機構とプロトン移動 (2015)
  • Author(s): 樋口芳樹
  • Organizer: 平成２７年度前期物性研究所短期研究会 機能物性融合科学シリーズ（３）「反応と輸送」
  • Place of Presentation: 東京大学物性研究所（千葉県柏市）
  • Year and Date: 2015-06-25
  • Invited
• [Presentation] 水素を新しいエネルギー源とする新領域の構築 (2015)
  • Author(s): 樋口芳樹
  • Organizer: 第16回 豊田理化学研究所フェロー研究報告会
  • Place of Presentation: 豊田理化学研究所オープンコミュニティ（愛知県豊田市）
  • Year and Date: 2015-06-22
  • Invited
• [Presentation] [NiFe]ヒドロゲナーゼの光照射で生じるNi-L状態の分光学的研究 (2015)
  • Author(s): 太虎林，西川幸志，井上誠也，樋口芳樹，廣田俊
  • Organizer: 第４２回生体分子科学討論会
  • Place of Presentation: 高崎シティーギャラリー （ 群馬県高崎市）
  • Year and Date: 2015-06-12
• [Presentation] ヒドロゲナーゼの酸化的不活性化－速度論的解析に基づく回避戦略－ (2015)
  • Author(s): 宋 慶盛，西原宏史，樋口芳樹，北隅優希，白井 理，加納健司
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山大学（岡山県・岡山市）
  • Year and Date: 2015-03-28
• [Presentation] 鉄硫黄クラスターの酸化還元状態による[NiFe]ヒドロゲナーゼの活性サイクル制御 (2015)
  • Author(s): 太 虎林，西川幸志，井上誠也，樋口芳樹，廣田 俊
  • Organizer: 日本化学会第95春季年会
  • Place of Presentation: 日本大学 理工学部船橋キャンパス（千葉県・船橋市）
  • Year and Date: 2015-03-27
• [Presentation] ヒドロゲナーゼの反応水素が見えるか？ (2015)
  • Author(s): 樋口芳樹
  • Organizer: 日本物理学会 第70回年次大会
  • Place of Presentation: 早稲田大学 早稲田キャンパス（東京都・新宿区）
  • Year and Date: 2015-03-21
  • Invited
• [Presentation] Structure, function and evolution of [NiFe]-Hydrogenases (2014)
  • Author(s): Yoshiki Higuchi
  • Organizer: The International Conference on Artificial Photosynthesis 2014
  • Place of Presentation: 淡路夢舞台国際会議場（兵庫県・淡路市）
  • Year and Date: 2014-11-27
  • Invited
• [Presentation] ヒドロゲナーゼの性質の電気化学的解析と修飾用電極の探索 (2014)
  • Author(s): 窪田慎太郎，東間清和，山崎徹，矢澤哲夫，安川智之，水谷文雄，樋口芳樹
  • Organizer: ポーラログラフィーおよび電気分析化学討論会
  • Place of Presentation: 京都工芸繊維大学 60周年記念館（京都府・京都市）
  • Year and Date: 2014-11-16
• [Presentation] Structural chemistry of oxygen tolerant [NiFe]-hydrogenase from Citrobacter S-77 (2014)
  • Author(s): Noor Dina Muhd Noor，Koji Nishikawa， Ki-Seok Yoon， Seiji Ogo，
  • Organizer: 日本結晶学会2014年度年会
  • Place of Presentation: 東京大学・弥生講堂（東京都・文京区）
  • Year and Date: 2014-11-01 – 2014-11-03
• [Presentation] 中性子結晶構造解析に向けた巨大単結晶の調製～相図を用いたアプローチ～ (2014)
  • Author(s): 井上誠也，西川幸志，玉田太郎，黒木良太，樋口芳樹
  • Organizer: 日本結晶学会2014年度年会
  • Place of Presentation: 東京大学・弥生講堂（東京都・文京区）
  • Year and Date: 2014-11-01 – 2014-11-03
• [Presentation] Towards structural determination of oxygen-stable [NiFe]-hydrogenase from Citrobacter S-77 (2014)
  • Author(s): Noor Dina Muhd Noor， Koji Nishikawa， Ki-Seok Yoon， Seiji Ogo， Yoshiki Higuchi
  • Organizer: 2nd International Picobiology Institute Symposium
  • Place of Presentation: 兵庫県立先端科学技術支援センター（兵庫県・上郡町）
  • Year and Date: 2014-10-09
• [Presentation] SAM 修飾電極を用いたシトクロム c3 単分子の電気伝導測定 (2014)
  • Author(s): 角田 早，蔡 徳七，鈴木 雅之，樋口 芳樹，松本 卓也
  • Organizer: 第8回分子科学討論会
  • Place of Presentation: 広島大学（広島県・広島市）
  • Year and Date: 2014-09-24
• [Presentation] [NiFe]ヒドロゲナーゼの活性化サイクルにおける新規中間体の特定 (2014)
  • Author(s): 太 虎林， 西川幸志， 樋口芳樹， 廣田 俊
  • Organizer: 第8回分子科学討論会
  • Place of Presentation: 広島大学（広島県・広島市）
  • Year and Date: 2014-09-23
• [Presentation] X-Ray Structural Studies of [NiFe]-Hydrogenases (2014)
  • Author(s): Yoshiki Higuchi
  • Organizer: International Conference of Catalytic Systems for Chemical Energy Conversion
  • Place of Presentation: Muelheim (Germany)
  • Year and Date: 2014-07-24
  • Invited
• [Presentation] 硫酸還元菌由来 [NiFe]ヒドロゲナーゼの光反応性の分光学的研究 (2014)
  • Author(s): 太 虎林，西川幸志，鈴木雅之，樋口 芳樹，廣田 俊
  • Organizer: 第14回日本蛋白質科学会年会
  • Place of Presentation: ワークピア横浜/横浜産貿易ホールマリネリア（神奈川県・横浜市）
  • Year and Date: 2014-06-26
• [Presentation] ヒドロゲナーゼの構造・機能・進化 (2014)
  • Author(s): 樋口芳樹
  • Organizer: 第4１回 生体分子科学討論会
  • Place of Presentation: 九州大学西新プラザ（福岡県・福岡市）
  • Year and Date: 2014-06-06
  • Invited
• [Presentation] Structural chemistry of oxygen tolerant [NiFe]-hydrogenase from Citrobacter S-77 (2014)
  • Author(s): Noor Dina Muhd Noor， Koji Nishikawa， Ki-Seok Yoon， Seiji Ogo， Yoshiki Higuchi
  • Organizer: EMTECH-University of Hyogo Joint Colloquium 2014
  • Place of Presentation: University of Putra (Malaysia)
  • Year and Date: 2014-05-26
• [Presentation] Crystallization of single crystals of Multi-Copper Oxidases for neutron diffraction experiment (2014)
  • Author(s): M. Akter, H. Komori, N. Shibata, T. Sakurai, K. Kataoka, Y. Higuchi
  • Organizer: 第1回ピコバイロジー研究所国際シンポジウム
  • Place of Presentation: 理研XFEL大会議室（兵庫）
• [Presentation] Crystallization of [NiFe]-hydrogenase maturation protein complex HypA-HypB (2014)
  • Author(s): Y. Okawa, Y. Higuchi, and Y. Shomura
  • Organizer: 第1回ピコバイロジー研究所国際シンポジウム
  • Place of Presentation: 理研XFEL大会議室（兵庫）
• [Presentation] Purification and Crystallization of NAD+-reducing [NiFe]-hydrogenase (2014)
  • Author(s): H. Nakagawa, M. Taketa, M. Habukawa, K. Kihira, N. Shibata, H. Nishihara, K.-S. Yoon, S. Ogo, Ma. Ishii, Y. Igarashi, Y. Shomura and Y. Higuchi
  • Organizer: 第1回ピコバイロジー研究所国際シンポジウム
  • Place of Presentation: 理研XFEL大会議室（兵庫）
• [Presentation] Crystallization of extracellular domains of protocadherin, a neural cell adhesion protein (2014)
  • Author(s): S. Yoshida, T. Hirabayashi, T. Yagi, Y. Shomura and Y. Higuchi
  • Organizer: 第1回ピコバイロジー研究所国際シンポジウム
  • Place of Presentation: 理研XFEL大会議室（兵庫）
• [Presentation] [NiFe]ヒドロゲナーゼのX線結晶構造解析 (2014)
  • Author(s): 庄村康人
  • Organizer: 第27回日本放射光学会年会放射光科学合同シンポジウム
  • Place of Presentation: 広島国際会議場（広島）
  • Invited
• [Presentation] 結晶構造解析による水素分解酵素の反応機構解明に向けた試み (2014)
  • Author(s): 庄村康人，西川幸志， 井上誠也，玉田太郎，樋口 芳樹
  • Organizer: 蛋白質研究所セミナー
  • Place of Presentation: 大阪大学蛋白質研究所（大阪）
  • Invited
• [Presentation] 逆行性輸送因子による積荷認識の構造的基盤 (2014)
  • Author(s): 寺脇慎一，大塚寛貴，樋口芳樹，若松馨
  • Organizer: 第29回PFシンポジウム
  • Place of Presentation: つくば国際会議場エポカル（茨城）
• [Presentation] H. thermoluteolus TH-1由来NAD+還元ヒドロゲナーゼの分光学研究 (2014)
  • Author(s): 太虎林，竹田翠，庄村康人，西川幸志，鈴木雅之，樋口芳樹，廣田俊
  • Organizer: 日本化学会第94春季年会
  • Place of Presentation: 名古屋大学東山キャンパス（愛知）
• [Presentation] 超好熱菌由来シトクロムcのドメインスワッピング (2014)
  • Author(s): 山中優，長尾聡，小森博文，樋口芳樹，廣田俊
  • Organizer: 日本化学会第94春季年会
  • Place of Presentation: 名古屋大学東山キャンパス（愛知）
• [Presentation] 大腸菌膜との相互作用によるシトクロムcの多量化 (2014)
  • Author(s): 林有吾，長尾聡，山中優，小森博文，樋口芳樹，廣田俊
  • Organizer: 日本化学会第94春季年会
  • Place of Presentation: 名古屋大学東山キャンパス（愛知）
• [Presentation] Reaction Mechanism of S-Carbamoylation of HypE by HypF in the Maturation of [NiFe]-hydrogenase (2013)
  • Author(s): Y. Higuchi and Y. Shomura
  • Organizer: 10th International Hydrogenase Conference
  • Place of Presentation: Szeged (Hungry)
  • Invited
• [Presentation] Domain Swapping of the Heme and N-Terminal α-Helix in Hydrogenobacter thermophilus Cytochrome-c552 Dimer (2013)
  • Author(s): Y. Hayashi, S. Nagao, H. Osuka, H. Komori, Y. Higuchi, and S. Hirota
  • Organizer: The 27th Annual Symposium of The Protein Society
  • Place of Presentation: Boston（USA）
• [Presentation] Structure of the native Axin-DIX domain crystallized without mercury compound. (2013)
  • Author(s): N. Shibata, T. Katsutani, S. Terawaki, H. Yamamoto, A. Kikuchi and Y.Higuchi
  • Organizer: 7th International Conference on Structural Genomics 2013
  • Place of Presentation: 京王プラザホテル札幌（北海道）
• [Presentation] αフルオロメチルヒスチジンによるヒスタミン合成酵素の阻害機構の解明 (2013)
  • Author(s): 小森博文，新田陽子，植野洋志，樋口芳樹
  • Organizer: 第86回日本生化学大会
  • Place of Presentation: パシフィコ横浜（神奈川）
• [Presentation] AFM によるシトクロムc3分子の電気伝導測定 (2013)
  • Author(s): 角田早，山口晴正，蔡徳七，平野義明，鈴木雅之，樋口芳樹，松本卓也
  • Organizer: 第 74 回応用物理学会
  • Place of Presentation: 同志社大学（京都）
• [Presentation] シトクロムc3ネットワークの電気特性 (2013)
  • Author(s): 山口晴正，角田早，蔡徳七，平野義明，鈴木雅之，樋口芳樹，松本卓也
  • Organizer: 第 74 回応用物理学会
  • Place of Presentation: 同志社大学（京都）
• [Presentation] シトクロムc3ネットワークの電気特性 (2013)
  • Author(s): 山口晴正，角田早，蔡徳七，平野義明，鈴木雅之，樋口芳樹，松本卓也
  • Organizer: 第7回分子科学討論会
  • Place of Presentation: 京都テルサ（京都）
• [Presentation] AFMによるシトクロムc3分子の電気伝導測定 (2013)
  • Author(s): 角田早，山口晴正，蔡徳七，平野義明，鈴木雅之，樋口芳樹，松本卓也
  • Organizer: 第7回分子科学討論会
  • Place of Presentation: 京都テルサ（京都）
• [Presentation] 水素-化学エネルギー変換・ヒドロゲナーゼの構造化学 (2013)
  • Author(s): 中川英恵，竹田翠，庄村康人，樋口芳樹
  • Organizer: 兵庫県立大学シンポジウム
  • Place of Presentation: 神戸市産業振興センター（兵庫）
• [Presentation] 水素分解酵素の成熟化機構の解明 (2013)
  • Author(s): 大川雄嗣，樋口芳樹，庄村康人
  • Organizer: 兵庫県立大学シンポジウム
  • Place of Presentation: 神戸市産業振興センター（兵庫）
• [Presentation] 糖質加水分解酵素ファミリー45に属する担子菌由来エンドグルカナーゼの構造・機 能解析 (2013)
  • Author(s): 中村彰彦，石田卓也，伊中浩治，樋口芳樹，伏信進矢，五十嵐圭日子，鮫島正浩
  • Organizer: 日本応用糖質科学会平成25年度大会（第62回）
  • Place of Presentation: 鹿児島大学（鹿児島）
• [Presentation] 超好熱菌由来シトクロムcの多量化と二量体構造 (2013)
  • Author(s): 山中優，長尾聡，羽田正雄，小森博文，樋口芳樹，廣田俊
  • Organizer: 第7回バイオ関連シンポジウム（日本化学会）
  • Place of Presentation: 名古屋大学豊田講堂（愛知）
• [Presentation] ヒスタミン合成酵素の構造解析 (2013)
  • Author(s): 小森博文，新田陽子，植野洋志，樋口芳樹
  • Organizer: 平成25年度日本結晶学会
  • Place of Presentation: 熊本大学（熊本）
• [Presentation] Preparation of single crystals of multi-copper oxidases suitable for neutron diffraction (2013)
  • Author(s): M. Akter，H. Komori，N. Shibata，Y. Shomura，T. Sakurai，K. Kataoka，Y. Higuchi
  • Organizer: 平成25年度日本結晶学会
  • Place of Presentation: 熊本大学（熊本）
• [Presentation] [NiFe]-ヒドロゲナーゼ成熟化タンパク質HypAの結晶化 (2013)
  • Author(s): 大川雄嗣，樋口芳樹，庄村康人
  • Organizer: 平成25年度日本結晶学会
  • Place of Presentation: 熊本大学（熊本）
• [Presentation] 細胞接着分子プロトカドヘリン細胞外領域の結晶化 (2013)
  • Author(s): 吉田早希，平林敬浩，八木健，庄村康人，樋口 芳樹
  • Organizer: 平成25年度日本結晶学会
  • Place of Presentation: 熊本大学（熊本）
• [Presentation] NAD+還元型ヒドロゲナーゼの結晶学的研究 (2013)
  • Author(s): 中川英恵，竹田翠，土生川真央，木平清人，柴田直樹
  • Organizer: 平成25年度日本結晶学会年会
  • Place of Presentation: 熊本大学（熊本）
• [Presentation] 水銀化合物非存在下での野生型 Axin DIX-ドメイン (2013)
  • Author(s): 勝谷拓也，柴田直樹，寺脇慎一，山本英樹，菊池章，樋口芳樹
  • Organizer: 平成25年度日本結晶学会
  • Place of Presentation: 熊本大学（熊本）
• [Presentation] Formation of Domain-Swapped Oligomeric Cytochrome c from Its Molten Globule State (2013)
  • Author(s): M. S. Deshpande, P. P. Parui, M. Yamanaka, S. Nagao, H. Kamikubo, M. Kataoka, H. Komori, Y. Higuchi and S. Hirota
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 国立京都国際会館（京都）
• [Presentation] X-ray Structural Study of O2-tolerant [NiFe] hydrogenase (2013)
  • Author(s): Y. Higuchi, K.-S. Yoon, H. Nishihara and Y. Shomura
  • Organizer: International Conference on Bio/Mimetic Solar Energy Conversion (iSEC)2013
  • Place of Presentation: 大阪市立大学（大阪）
  • Invited
• [Presentation] Polymerization and Oligomerization of Cytochrome c and Myoglobin by Domain Swapping (2013)
  • Author(s): S. Hirota, S. Nagao, Y. Hayashi, Z. Wang, A. D. Nugraheni and Y. Higuchi
  • Organizer: 12th International Symposium on Applied Bioinorganic Chemistry
  • Place of Presentation: Guangzhou （China）
  • Invited
• [Book] 実験医学増刊 構造生命科学 (2014)
  • Author(s): 樋口芳樹
  • Total Pages: 230
  • Publisher: 羊土社
• [Book] 進化を続ける構造生物学，松島正明，伊中浩治編 (2014)
  • Author(s): 樋口芳樹
  • Total Pages: 216
  • Publisher: 化学同人
• [Book] CSJカレントレビュー15，次世代のバイオ水素エネルギー開発 (2014)
  • Author(s): 庄村康人，樋口芳樹
  • Total Pages: 174
  • Publisher: 化学同人
• [Book] 光合成のエネルギー利用と環境応用 (2014)
  • Author(s): 樋口芳樹
  • Total Pages: 308
  • Publisher: CMC出版