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Analysis of time constant encoded in circadian clock proteins

Research Project

Project/Area Number 25291039
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Biophysics
Research InstitutionInstitute for Molecular Science

Principal Investigator

AKIYAMA Shuji  分子科学研究所, 協奏分子システム研究センター, 教授 (50391842)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Keywords概日時計 / 生物時計 / タンパク質 / タイミング
Outline of Final Research Achievements

This research project aims at analyzing pre-steady-state relaxations of the ATPase activity of a clock protein, KaiC, which is known to be a pacemaker of the circadian clock system in cyanobacteria. A fine correlation between the relaxation time of KaiC alone and the clock-system’s frequency supports the idea that the slow ATPase in KaiC yields the circadian response frequency of intermolecular interactions with other clock-related proteins.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (20 results)

All 2016 2015 2014 Other

All Int'l Joint Research (1 results) Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 3 results) Presentation (12 results) (of which Int'l Joint Research: 4 results,  Invited: 9 results) Remarks (1 results)

  • [Int'l Joint Research] PSL Research Univ., Chimie ParisTech(France)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Absolute slowness encoded in the circadian clock protein KaiC2016

    • Author(s)
      Abe J, Mukaiyama A, Akiyama S
    • Journal Title

      SPring-8 Research Frontiers 2015

      Volume: 印刷中

    • Related Report
      2015 Annual Research Report
    • Open Access
  • [Journal Article] 時間生物学と放射光科学の接点2016

    • Author(s)
      秋山 修志
    • Journal Title

      放射光

      Volume: 印刷中

    • NAID

      40020850505

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 時計タンパク質KaiCの「遅さ」が刻み込まれた原子構造2016

    • Author(s)
      阿部 淳,向山 厚,秋山 修志
    • Journal Title

      SPring-8/SACLA利用者情報

      Volume: 21 Pages: 2-4

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Atomic-scale origins of slowness in the cyanobacterial circadian clock2015

    • Author(s)
      J. Abe, T. B. Hiyama, A. Mukaiyama, S. Son, T. Mori, S. Saito, M. Osako, J. Wolanin, E. Yamashita, T. Kondo, S. Akiyama
    • Journal Title

      Science

      Volume: 349 Issue: 6245 Pages: 312-316

    • DOI

      10.1126/science.1261040

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] タンパク質の化学反応が細胞内の時を計る2015

    • Author(s)
      向山 厚,阿部 淳,孫 世永,秋山 修志
    • Journal Title

      実験医学

      Volume: 33 Pages: 3119-3122

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A protocol for preparing nucleotide-free KaiC monomer2015

    • Author(s)
      Mukaiyama, A., Osako, M., Hikima, T., Kondo, T., and Akiyama, S.
    • Journal Title

      BIOPHYSICS

      Volume: 11 Issue: 0 Pages: 79-84

    • DOI

      10.2142/biophysics.11.79

    • NAID

      130004940817

    • ISSN
      1349-2942
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] X-ray Solution Scattering as Research Tools for Bio-molecular Systems2016

    • Author(s)
      Akiyama S
    • Organizer
      Winter School of ASIA CORE Program
    • Place of Presentation
      Beijing, China
    • Year and Date
      2016-02-26
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Circadian periodicity encoded in KaiC ATPase2016

    • Author(s)
      Mukaiyama A, Abe J, and Akiyama S
    • Organizer
      Winter School of ASIA CORE Program
    • Place of Presentation
      Beijing, China
    • Year and Date
      2016-02-26
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Atomic-scale origins of slowness in the cyanobacterial circadian clock2016

    • Author(s)
      Akiyama S
    • Organizer
      8th Japan-Korea Seminars on Biomolecular Science
    • Place of Presentation
      岡崎コンファレンスセンター(愛知県岡崎市)
    • Year and Date
      2016-02-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 時計タンパク質 KaiC に書き込まれた生物時計の発振周期2015

    • Author(s)
      向山 厚, 阿部 淳, 檜山 卓也, 孫 世永, Wolanin Julie , 山下 栄樹, 近藤 孝男, 秋山 修志
    • Organizer
      第22回日本時間生物学会学術大会
    • Place of Presentation
      東京大学本郷キャンパス(東京都文京区)
    • Year and Date
      2015-11-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] 藍藻の時計タンパク質に内包された概日周期と遅さの根源2015

    • Author(s)
      秋山 修志
    • Organizer
      藍藻の分子生物学2015
    • Place of Presentation
      かずさアカデミアホール(千葉県木更津市)
    • Year and Date
      2015-11-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] KaiC as Circadian Pacemaker of Cyanobacterial Circadian Clock2015

    • Author(s)
      Akiyama S
    • Organizer
      European Biological Rhythms Society (EBRS)/World Congress of Chronobiology (WCC) meeting
    • Place of Presentation
      Manchester, UK
    • Year and Date
      2015-08-06
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 蛍光プローブの挿入による時計タンパク質KaiCの周期的構造変化の計測2015

    • Author(s)
      向山 厚,秋山 修志
    • Organizer
      第42回生体分子科学討論会
    • Place of Presentation
      高崎シティーギャラリー(群馬県高松市)
    • Year and Date
      2015-06-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] 概日時計システム研究におけるbioSANSへの期待と展望2015

    • Author(s)
      秋山修志
    • Organizer
      第3回Neutrons in Biology研究会
    • Place of Presentation
      日本原子力研究開発機構・原子力科学研究所 (茨城県那珂郡)
    • Year and Date
      2015-03-31
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 会議報告、ガイドラインに関する査読者とのやり取り2014

    • Author(s)
      秋山修志
    • Organizer
      分子システム研究における溶液散乱
    • Place of Presentation
      分子科学研究所(愛知県岡崎市)
    • Year and Date
      2014-12-20
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Watching slow but ordered dynamics of clock proteins2014

    • Author(s)
      Shuji Akiyama
    • Organizer
      第21回日本時間生物学会学術大会
    • Place of Presentation
      九州大学(福岡県福岡市)
    • Year and Date
      2014-11-08
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 概日時計システム研究におけるbioSANSへの期待と展望2014

    • Author(s)
      秋山修志
    • Organizer
      平成26年度第1回生物構造学研究会
    • Place of Presentation
      エッサム神田ホール401会議室 (東京都千代田区)
    • Year and Date
      2014-10-03
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] KaiC as Circadian Pacemaker of Cyanobacterial Circadian Clock2014

    • Author(s)
      Akiyama S
    • Organizer
      Society for Research on Biological Rhythms
    • Place of Presentation
      Montana,USA
    • Year and Date
      2014-06-17
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Remarks] AKIYAMA Lab.

    • URL

      http://bms.ims.ac.jp/AkiyamaG/index.html

    • Related Report
      2013 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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