• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Stress and amino acid-responsive mechanisms of TORC1 regulation

Research Project

Project/Area Number 25291042
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Cell biology
Research InstitutionThe University of Tokyo

Principal Investigator

Maeda Tatsuya  東京大学, 分子細胞生物学研究所, 准教授 (90280627)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2015: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
KeywordsTOR / TORC1 / ラパマイシン / アミノ酸 / ストレス顆粒 / グルタミン / 細胞内情報伝達 / target of rapamycin / TOR complex 1 / アミノ酸栄養 / PI3キナーゼ / 栄養
Outline of Final Research Achievements

We have developed a TORC1 kinase assay, by using a membrane-preserved TORC1 preparation, that reproduces for the first time, to the best of our knowledge, the amino acid-responsive TORC1 activation in vitro. The amino acid-responsiveness of the assay system was shown to be independent of the well-known Gtr-dependent nutrient-responsive mechanism and to involve the Vps34 PI3 kinase and the Pib2 protein containing a PI(3)P-binding FYVE domain.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (22 results)

All 2016 2015 2014 2013 Other

All Journal Article (8 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 8 results,  Open Access: 4 results,  Acknowledgement Compliant: 2 results) Presentation (14 results) (of which Invited: 2 results)

  • [Journal Article] The transcription factor Bach2 is phosphorylated at multiple sites in murine B cells but a single site prevents its nuclear localization2016

    • Author(s)
      Ando R, Shima H, Tamahara T, Sato Y, Watanabe-Matsui M, Kato H, Sax N, Motohashi H, Taguchi K, Yamamoto M, Nio M, Maeda T, Ochiai K, Muto A, Igarashi K
    • Journal Title

      J Biol Chem

      Volume: 291 Issue: 4 Pages: 1826-1840

    • DOI

      10.1074/jbc.m115.661702

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)2016

    • Author(s)
      Klionsky DJ., Abdelmohsen K., Abe A., Abedin MJ.,Inomata M., et al.
    • Journal Title

      Autophagy

      Volume: 12(1) Issue: 1 Pages: 1-222

    • DOI

      10.1080/15548627.2015.1100356

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Conserved Mode of Interaction between Yeast Bro1 Family V Domains and YP(X)nL Motif-Containing Target Proteins2015

    • Author(s)
      Yoko Kimura, Mirai Tanigawa, Junko Kawawaki, Kenji Takagi, Tsunehiro Mizushima, Tatsuya Maeda, Keiji Tanaka
    • Journal Title

      Eukaryotic Cell

      Volume: 14 Issue: 10 Pages: 976-982

    • DOI

      10.1128/ec.00091-15

    • NAID

      120005671359

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Endolysosomal membrane trafficking complexes drive nutrient-dependent TORC1 signaling to control cell growth in Saccharomyces cerevisiae.2014

    • Author(s)
      Kingsbury JM
    • Journal Title

      Genetics

      Volume: 196 Issue: 4 Pages: 1077-1089

    • DOI

      10.1534/genetics.114.161646

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Selective Activation of mTORC1 Signaling Recapitulates Microcephaly, Tuberous Sclerosis, and Neurodegenerative Diseases2014

    • Author(s)
      Kassai H, Sugaya Y, Noda S, Nakao K, Maeda T, Kano M, Aiba A
    • Journal Title

      Cell Reports

      Volume: 7 Issue: 5 Pages: 1626

    • DOI

      10.1016/j.celrep.2014.04.048

    • URL

      https://pure.teikyo.jp/en/publications/d714d3c6-814c-403f-81f6-3d5a4a38414b

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Changes in cell morphology are coordinated with cell growth through the TORC1 pathway.2013

    • Author(s)
      Goranov AI
    • Journal Title

      Curr Biol.

      Volume: 23 Issue: 14 Pages: 1269-1279

    • DOI

      10.1016/j.cub.2013.05.035

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hyper-Activation of the Target of Rapamycin (Tor) Kinase 1 Decreases Intracellular Glutathione Content in <i>Saccharomyces cerevisiae</i> as Revealed by LC-MS/MS Analysis2013

    • Author(s)
      Oku M
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 77 Issue: 7 Pages: 1608-1611

    • DOI

      10.1271/bbb.130314

    • NAID

      10031190482

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of histidine-aspartate kinase HK1 and identification of histidine phosphotransfer proteins as potential partners in a Populus multistep phosphorelay2013

    • Author(s)
      Hericourt F, Chefdor F, Bertheau L, Tanigawa M, Maeda T, Guirimand G, Courdavault V, Larcher M, Depierreux C, Benedetti H, Morabito D, Brignolas F, Carpin S
    • Journal Title

      Physiol Plant.

      Volume: 149 Issue: 2 Pages: 188-199

    • DOI

      10.1111/ppl.12024

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] Rag/Gtr複合体非依存的TORC1活性化機構の解析2016

    • Author(s)
      谷川美頼、前田達哉
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-03-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] 酵母Bro1 VドメインとYP(X)nLモチーフを持つターゲットタンパク質との相互作用の解析2015

    • Author(s)
      木村洋子、谷川美頼、川脇純子、高木賢治、水島恒裕、前田達哉、田中 啓二
    • Organizer
      第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] 清酒酵母におけるTORC1シグナリングとアルコール発酵の関連に関する研究2015

    • Author(s)
      渡辺大輔、周延、陳佳文、水野恵、荒木義雄、杉本幸子、万クン、中沢伸重、赤尾健、下飯仁、水田啓子、前田達哉、高木博史
    • Organizer
      第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Year and Date
      2015-12-02
    • Related Report
      2015 Annual Research Report
  • [Presentation] TORC1のin vitro活性評価法の開発2015

    • Author(s)
      谷川美頼、前田達哉
    • Organizer
      清酒酵母におけるTORC1シグナリングとアルコール発酵の関連に関する研究
    • Place of Presentation
      広島大学東広島キャンパス(広島県東広島市)
    • Year and Date
      2015-09-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 清酒酵母におけるTORC1シグナリングとアルコール発酵の関連に関する研究2015

    • Author(s)
      渡辺大輔、周延、陳佳文、水野恵、荒木義雄、杉本幸子、万クン、中沢伸重、赤尾健、下飯仁、水田啓子、前田達哉、高木博史
    • Organizer
      酵母遺伝学フォーラム第48回研究報告会
    • Place of Presentation
      広島大学東広島キャンパス(広島県東広島市)
    • Year and Date
      2015-08-31
    • Related Report
      2015 Annual Research Report
  • [Presentation] 酵母TORC1のin vitroでの活性評価法の開発2015

    • Author(s)
      谷川美頼
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山大学・津島キャンパス
    • Year and Date
      2015-03-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] The role of glutamine in the activation of TORC1 in yeast2014

    • Author(s)
      陳佳文
    • Organizer
      酵母遺伝学フォーラム第47会研究報告会
    • Place of Presentation
      東京大学・弥生キャンパス・弥生講堂(東京)
    • Year and Date
      2014-09-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] HOG経路によるcytokinesisの制御2014

    • Author(s)
      谷川美頼
    • Organizer
      酵母遺伝学フォーラム第47会研究報告会
    • Place of Presentation
      東京大学・弥生キャンパス・弥生講堂(東京)
    • Year and Date
      2014-09-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] 細胞質分裂におけるHOG経路の機能

    • Author(s)
      谷川美頼
    • Organizer
      酵母遺伝学フォーラム第46回研究報告会
    • Place of Presentation
      東北学院大学土樋キャンパス(宮城)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 出芽酵母のアルコール発酵調節におけるTORシグナリングの意義

    • Author(s)
      渡辺大輔
    • Organizer
      酵母遺伝学フォーラム第46回研究報告会
    • Place of Presentation
      東北学院大学土樋キャンパス(宮城)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Ther regulatory effect of glutamine on TORC1 activity in yeast

    • Author(s)
      陳佳文
    • Organizer
      酵母遺伝学フォーラム第46回研究報告会
    • Place of Presentation
      東北学院大学土樋キャンパス(宮城)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ロイシルtRNA合成酵素を介したTORC1活性制御の検証

    • Author(s)
      豊水理恵
    • Organizer
      酵母遺伝学フォーラム第46回研究報告会
    • Place of Presentation
      東北学院大学土樋キャンパス(宮城)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ストレスに応答したTOR複合体1(TORC1)制御機構

    • Author(s)
      前田達哉
    • Organizer
      第7回レドックス・ライフイノベーションシンポジウム
    • Place of Presentation
      東京大学大学院農学生命科学研究科(東京)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ストレスと栄養に応答したTORC1活性制御

    • Author(s)
      前田達哉
    • Organizer
      大阪大学蛋白質研究所セミナー「キナーゼ・シグナリング研究の進展」
    • Place of Presentation
      大阪大学蛋白質研究所(大阪)
    • Related Report
      2013 Annual Research Report
    • Invited

URL: 

Published: 2013-05-21   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi