| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2015: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
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| Outline of Final Research Achievements |
We have developed a TORC1 kinase assay, by using a membrane-preserved TORC1 preparation, that reproduces for the first time, to the best of our knowledge, the amino acid-responsive TORC1 activation in vitro. The amino acid-responsiveness of the assay system was shown to be independent of the well-known Gtr-dependent nutrient-responsive mechanism and to involve the Vps34 PI3 kinase and the Pib2 protein containing a PI(3)P-binding FYVE domain.
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