Stress and amino acid-responsive mechanisms of TORC1 regulation
Project/Area Number |
25291042
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Cell biology
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Research Institution | The University of Tokyo |
Principal Investigator |
Maeda Tatsuya 東京大学, 分子細胞生物学研究所, 准教授 (90280627)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2015: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
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Keywords | TOR / TORC1 / ラパマイシン / アミノ酸 / ストレス顆粒 / グルタミン / 細胞内情報伝達 / target of rapamycin / TOR complex 1 / アミノ酸栄養 / PI3キナーゼ / 栄養 |
Outline of Final Research Achievements |
We have developed a TORC1 kinase assay, by using a membrane-preserved TORC1 preparation, that reproduces for the first time, to the best of our knowledge, the amino acid-responsive TORC1 activation in vitro. The amino acid-responsiveness of the assay system was shown to be independent of the well-known Gtr-dependent nutrient-responsive mechanism and to involve the Vps34 PI3 kinase and the Pib2 protein containing a PI(3)P-binding FYVE domain.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] The transcription factor Bach2 is phosphorylated at multiple sites in murine B cells but a single site prevents its nuclear localization2016
Author(s)
Ando R, Shima H, Tamahara T, Sato Y, Watanabe-Matsui M, Kato H, Sax N, Motohashi H, Taguchi K, Yamamoto M, Nio M, Maeda T, Ochiai K, Muto A, Igarashi K
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Journal Title
J Biol Chem
Volume: 291
Issue: 4
Pages: 1826-1840
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Characterization of histidine-aspartate kinase HK1 and identification of histidine phosphotransfer proteins as potential partners in a Populus multistep phosphorelay2013
Author(s)
Hericourt F, Chefdor F, Bertheau L, Tanigawa M, Maeda T, Guirimand G, Courdavault V, Larcher M, Depierreux C, Benedetti H, Morabito D, Brignolas F, Carpin S
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Journal Title
Physiol Plant.
Volume: 149
Issue: 2
Pages: 188-199
DOI
Related Report
Peer Reviewed
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