Analyses of the onset of embryonic blood circulation as multi-step processes
| Project/Area Number |
25291051
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Developmental biology
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2013-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2015: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
|
|---|
| Keywords | ADAMプロテアーゼ / 血管形成 / 血液循環 / 造血 / ゼブラフィッシュ / ライブイメージング / 再生 / インテグリン |
|---|
| Outline of Final Research Achievements |
We investigated roles of ADAM8 in blood development and in inflammatory reactions prior to regenerative myogenesis, by which injured myofibers are eliminated to make new one. No evident defects were observed in ADAM8 knockout (KO) mice. We showed that efficient elimination of injured myofibers during regeneration requires ADAM8. Upon cardiotoxin-induced skeletal muscle injury, neutrophils invade into myofibers through the basement membrane and cluster in wild type, but not in ADAM8 KO mice although neutrophils of the latter infiltrate into interstitial tissues similarly to those of wild type mice. Neutrophils lose their adhesiveness to blood vessels after infiltration, which involves an ectodomain shedding of PSGL-1 on their surface. Expression of PSGL-1 on the surface of neutrophils remain higher in ADAM8 KO than in wild type mice, suggesting that ADAM8 mediates the invasion of neutrophils by the removal of their adhesiveness to blood vessels after infiltration into injured muscle.
|
Report
(5 results)
Research Products
(52 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Roles of ADAM8 for skeletal muscle regeneration2016
Author(s)
Daigo Nishimura , Hiroshi Sakai , Takahiko Sato , Fuminori Sato , Satoshi Nishimura ,Noriko Toyama-Sorimachi , J.W. Bartsch , Atsuko Sehara-Fujisawa
Organizer
第24回マクロファージ分子細胞生物学会国際シンポジウム（MMCB2016）
Place of Presentation
東京都千代田区
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] What can we learn by exploring the interstitial space of skeletal muscle during development and regeneration?2015
Author(s)
Sato F, Nishimura D, Hori S, Arai H, Hiramuki Y, Sogabe M, Kuriki M, Choi M, Wang Z, Kawahara A, Kawakami K, Sehara-Fujisawa A
Organizer
48th Annual meeting of the Japanese Society of Developmental Biologists
Place of Presentation
つくば市
Related Report
-
-
-
-
-
[Presentation] Mechanisms of Skeletal Muscle Regeneration.2014
Author(s)
Atsuko Sehara-Fujisawa
Organizer
Symposium “Genomic and epigenomic insights into vertebrate regeneration, development and evolustion - development and evolution - Xenopus and fish as models”
Place of Presentation
Pontificia Universidad Catolica de Chile（チリ・カトリカ大学）
Year and Date
2014-11-05
Related Report
Invited
-
-
-
-
-
-
-
-
-
-
-
-
-
