| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
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| Outline of Final Research Achievements |
Sperm and oocyte are highly specified cells to transfer genetic information to next generations, but they attain totipotency by reprograming soon after fertilization. The reprogramming process includes protamine-histone exchange, DNA demethylation, zygotic gene activation, and degradation of maternal RNAs and proteins, and so on. However, mechanisms for acquisition of totipotency are largely unclear despite their biological significance. In this study, we identified the genes specifically expressed in totipotent cells. Among these totipotent-cell-specific genes, we found that Klf17 (Kruppel-like factor 17) and Btg4 (B cell translocation 4) are maternal effect genes required for pre-implantation development. Furthermore, we revealed that Klf17 and Btg4 are involved in zygotic genome activation and clearance of maternal transcripts after fertilization, respectively.
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