Mechanisms controlling the multilevel polarity formation in the mouse oviduct

• Project/Area Number: 25291054
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: National Institute for Basic Biology
• Principal Investigator: FUJIMORI Toshihiko 基礎生物学研究所, 初期発生研究部門, 教授 (80301274)
• Co-Investigator(Kenkyū-buntansha): 小林 徹也 東京大学, 生産技術研究所, 准教授 (90513359)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2013: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
• Keywords: マウス / 卵管 / 細胞極性

Outline of Final Research Achievements

Ovulated oocytes are transported in oviducts by unidirectional motility of multi-cilia and the resultant secretory fluid flow from ovary to uterus. The mammalian oviduct is highly polarized, where the epithelium forms longitudinal folds along the ovary-uterus axis, epithelial cells are elongated along the axis, and the epithelial multicilia beat towards the uterus to transport the ovulated ova. These cellular orientations in the oviduct are reflecting planar cell polarity (PCP). We studied mechanisms of the multilevel polarity formation process in the mouse oviduct by focusing on the function of PCP　factors.

Research Products

• [Int'l Joint Research] Univ. of Louvain(ベルギー)
• [Int'l Joint Research] Harvard Univ.(米国)
• [Int'l Joint Research] Univ. of Louvain(ベルギー)
• [Int'l Joint Research] Harvard Univ.(米国)
• [Int'l Joint Research] ルーヴァンカトリック大学(ベルギー)
• [Journal Article] Distinct intracellular Ca2+ dynamics regulate apical constriction and differentially contribute to neural tube closure. (2017)
  • Author(s): Suzuki, M., Sato, M., Koyama, H., Hara, Y., Hayashi, K., Yasue, N., Imamura, H., Fujimori, T., Nagai, T., Campbell, RE., Ueno, N.
  • Journal Title: Development. Volume: 144 Issue: 7 Pages: 1307-1316
  • DOI: 10.1242/dev.141952
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A Wnt5 Activity Asymmetry and Intercellular Signaling via PCP Proteins Polarize Node Cells for Left-Right Symmetry Breaking. (2017)
  • Author(s): Minegishi, K., Hashimoto, M., Ajima, R., Takaoka, K., Shinohara, K., Ikawa, Y., Nishimura, H., McMahon, AP., Willert, K., Okada, Y., Sasaki, H., Shi, D., Fujimori, T., Ohtsuka, T., Igarashi, Y., Yamaguchi, TP., Shimono, A., Shiratori, H., Hamada, H.
  • Journal Title: Dev Cell. Volume: 40 Issue: 5 Pages: 439-452
  • DOI: 10.1016/j.devcel.2017.02.010
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Dynamics of planar cell polarity protein Vangl2 in the mouse oviduct epithelium. (2016)
  • Author(s): Shi, D., Usami, F., Komatsu, K., Oka, S., Abe, T., Uemura, T., and Fujimori, T.
  • Journal Title: Mech Dev. Volume: 141 Pages: 78-89
  • Peer Reviewed / Open Access
• [Journal Article] 卵の取り込みと受精卵の輸送 (2016)
  • Author(s): 石東博、小松紘司、藤森俊彦
  • Journal Title: 臨床婦人科産科 Volume: 9 Pages: 810-816
• [Journal Article] Celsr1 is required for the generation of polarity at multiple levels of the mouse oviduct. (2014)
  • Author(s): Shi D, Komatsu K, Hirao M, Toyooka Y, Koyama H, Tissir F, Goffinet AM, Uemura T, Fujimori T.
  • Journal Title: Development Volume: 141 Pages: 4558-4558
  • NAID: 120006360788
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Mechanical control of notochord morphogenesis by extra-embryonic tissues in mouse embryos (2014)
  • Author(s): Yu Imuta, Hiroshi Koyama, Dongbo Shi, Mototsugu Eiraku, Toshihiko Fujimori, Hiroshi Sasaki
  • Journal Title: Mechanisms of Development Volume: 132 Pages: 44-58
  • DOI: 10.1016/j.mod.2014.01.004
  • Peer Reviewed / Open Access
• [Journal Article] (Preview) Atypical Cadherin Negotiates a Turn (2013)
  • Author(s): Dongbo Shi, Toshihiko Fujimori, and Tadashi Uemura
  • Journal Title: Developmental Cell Volume: 26 Issue: 1 Pages: 1-3
  • DOI: 10.1016/j.devcel.2013.06.024
  • Peer Reviewed
• [Presentation] Cell polarity formation during the mouse oviduct development. (2017)
  • Author(s): Toshihiko Fujimori
  • Organizer: Joint Meeting of the German and Japanese Societies of Developmental Biologists
  • Place of Presentation: University of Kiel, (Kiel, Germany)
  • Year and Date: 2017-03-15
  • Int'l Joint Research / Invited
• [Presentation] Mouse oviduct multicilia formation regulated by PCP factors (2016)
  • Author(s): Toshihiko Fujimori
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-11-30
  • Int'l Joint Research / Invited
• [Presentation] PCP in the mouse oviduct. (2015)
  • Author(s): Toshihiko Fujimori
  • Organizer: Finnish-Japanese joint symposium on Morphogenesis and Signaling
  • Place of Presentation: Helsinki, Finland
  • Year and Date: 2015-03-03
  • Int'l Joint Research / Invited
• [Presentation] PCP Pathway and Cellular Geometric Pattern in Oviduct Morphogenesis. (2014)
  • Author(s): Toshihiko Fujimori
  • Organizer: The 62nd NIBB Conference FORCE IN DEVELOPMENT
  • Place of Presentation: 岡崎コンファレンスセンター（愛知県岡崎市）
  • Year and Date: 2014-11-17
  • Int'l Joint Research / Invited
• [Presentation] Behaviors of cells and gene expression in early mouse development (2013)
  • Author(s): Toshihiko Fujimori
  • Organizer: Academia Sinica Lecture
  • Place of Presentation: Institute of Cellular and Organismic Biology, Academia Sinica, Taiwan
  • Invited
• [Presentation] Behaviors of Cells and Gene Expression in Early Mouse Development (2013)
  • Author(s): Toshihiko Fujimori
  • Organizer: The 61st NIBB Conference Cellular Community in Mammalian Embryogenesis
  • Place of Presentation: 岡崎コンファレンスセンター(愛知県岡崎市）
• [Book] The Cadherin Superfamily (2016)
  • Author(s): Shi, D., Arata M., Usui, T., Fujimori, T., Uemura, T.
  • Total Pages: 428
  • Publisher: Springer
• [Remarks] 基礎生物学研究所・初期発生研究部門
  • URL: http://www.nibb.ac.jp/embryo/index.html
• [Remarks] 基礎生物学研究所・初期発生研究部門
  • URL: http://www.nibb.ac.jp/embryo/
• [Remarks] 基礎生物学研究所 初期発生研究部門
  • URL: http://www.nibb.ac.jp/embryo/