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Study in the mechanism of differentiation and morphogenesis in plant cells by imaging quantitative analysis and simulation

Research Project

Project/Area Number 25291056
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionThe University of Tokyo

Principal Investigator

HASEZAWA Seiichiro  東京大学, 新領域創成科学研究科, 教授 (40172902)

Co-Investigator(Renkei-kenkyūsha) MATSUNAGA Sachihiro  東京理科大学, 理工学部, 教授 (40323448)
TAMAOKI Masanori  国立研究開発法人国立環境研究所, 生物・生態系環境研究センター, 主任研究員 (00311324)
KUTSUNA Natsumaro  東京大学, 大学院新領域創成科学研究科, 特任准教授 (70578559)
TAKAHASHI Shinya  筑波大学, 北アフリカ研究センター, 副主任研究員 (80370419)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Keywordsオルガネラ / 細胞骨格 / 細胞壁 / イメージング / 画像定量解析
Outline of Final Research Achievements

In this study, the intracellular structure-labeled transformed plants and imaging-quantitative analysis techniques have been developed. The cell morphogenesis and dynamics of the intracellular structures were analyzed by the image processing. The following four items were performed. (1) imaging-quantitative analysis of leaf epidermal cells, (2) analysis of artificial deformation and cell morphology in leaf epidermal cell wall, (3) analysis of the change in plant cell morphogenesis by high CO2 and UV-B treatments, (4) development of imaging-quantitative analysis techniques. Finally, a number of important knowledges, obtained for each item, have been published in many papers.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (28 results)

All 2016 2015 2014 2013 Other

All Journal Article (13 results) (of which Peer Reviewed: 13 results,  Open Access: 9 results,  Acknowledgement Compliant: 5 results) Presentation (15 results)

  • [Journal Article] Nuclear function of subclass I actin depolymerizing factor contributes to susceptibility in Arabidopsis to an adapted powdery mildew fungus.2016

    • Author(s)
      Inada N, Higaki T, Hasezawa S
    • Journal Title

      Plant Physiol

      Volume: 170 Issue: 3 Pages: 1420-1434

    • DOI

      10.1104/pp.15.01265

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Accumulation of fluorescent proteins derived from a trans-Golgi cisternal membrane marker and paramural bodies in interdigitated apoplastic space in Arabidopsis leaf epidermis2016

    • Author(s)
      Akita, K., Kobayashi, M., Sato, M., Kutsuna, N., Ueda, T., Toyooka, K., Nagata, N., Hasezawa, S., and Higaki, T.
    • Journal Title

      Protoplasma

      Volume: in press Issue: 1 Pages: 367-377

    • DOI

      10.1007/s00709-016-0955-1

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Simplification of vacuole structure during plant cell death triggered by culture filtrates of Erwinia carotovora.2015

    • Author(s)
      Hirakawa Y, Nomura T, Hasezawa S, Higaki T
    • Journal Title

      Journal of Integrated Plant Biology

      Volume: 57 Issue: 1 Pages: 127-135

    • DOI

      10.1111/jipb.12304

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Semi-automatic organelle detection on transmission electron microscopic images2015

    • Author(s)
      T. Higaki, N. Kutsuna, K. Akita, M. Sato, F. Sawaki, M. Kobayashi, N. Nagata, K. Toyooka, S. Hasezawa
    • Journal Title

      SCIENTIFIC REPORTS

      Volume: 5 Issue: 1 Pages: 7794-7794

    • DOI

      10.1038/srep07794

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Vacuole structure analysis during cell death subsequent to application of Erwinia carotovora culture filtrates to cell cultures of Nicotiana tabacum2015

    • Author(s)
      Hirakawa Y, Hasezawa S, Higaki T
    • Journal Title

      Bio Protoc

      Volume: 5

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Radiocesium distribution in bamboo shoots after the Fukushima nuclear accident.2014

    • Author(s)
      Higaki T, Higaki S, Hirota M, Hasezawa S
    • Journal Title

      PLOS ONE

      Volume: 9 Issue: 5 Pages: e97659-e97659

    • DOI

      10.1371/journal.pone.0097659

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dynamics and environmental responses of PATROL1 in Arabidopsis subsidiary cells.2014

    • Author(s)
      Higaki, T., Hashimoto-Sugimoto, M., Akita, K., Iba, K., and Hasezawa, S.
    • Journal Title

      Plant Cell Physiol.

      Volume: 55 Issue: 4 Pages: 773-780

    • DOI

      10.1093/pcp/pct151

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Wide-range high-resolution transmission electron microscopy reveals morphological and distributional changes of endomembrane compartments during log to stationary transition of growth phase in tobacco BY-2 cells.2014

    • Author(s)
      Toyooka K, Sato M, Kutsuna N, Higaki T, Sawaki F, Wakazaki M, Goto Y, Hasezawa S, Nagata N, Matsuoka K
    • Journal Title

      Plant Cell Physiol.

      Volume: 55 Issue: 9 Pages: 1544-1555

    • DOI

      10.1093/pcp/pcu084

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Breaking of the plant stomatal one-cell-spacing rule by sugar solution immersion.2013

    • Author(s)
      Akita K, Hasezawa S, Higaki T
    • Journal Title

      PLoS ONE

      Volume: 8 Issue: 9 Pages: e72456-e72456

    • DOI

      10.1371/journal.pone.0072456

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mechanism of microtubule array expansion in the cytokinetic phragmoplast.2013

    • Author(s)
      Murata, T., et al.
    • Journal Title

      Nat Commun

      Volume: Jun 17;4 Issue: 1 Pages: 1967-1967

    • DOI

      10.1038/ncomms2967

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An atypical tubulin kinase mediates stress-induced microtubule depolymer ization in Arabidopsis2013

    • Author(s)
      Satoshi Fujita, Jaromir Pytela, Takashi Hotta, Takehide Kato, Takahiro Hamada, Rie Akamatsu, Yasumasa Ishida, Natsumaro Kutsuna, Seiichiro Hasezawa, Yuko Nomura, Hirofumi Nakagami, Takashi Hashimoto
    • Journal Title

      Curr. Biol

      Volume: 23 Issue: 20 Pages: 1969-1978

    • DOI

      10.1016/j.cub.2013.08.006

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Munc13-like protein in Arabidopsis mediates H+-ATPase translocation that is essential for stomatal responses2013

    • Author(s)
      Hashimoto-Sugimoto, M., Higaki, T., Yaeno, T., Nagami, A., Irie, M., Fujimi M., Miyamoto, M., Akita, K., Negi, J., Shirasu, K., Hasezawa, S. and Iba, K.
    • Journal Title

      Nature Commun.

      Volume: 4 Issue: 1 Pages: 2215-2215

    • DOI

      10.1038/ncomms3215

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] LIPS database with LIPService: a microscopic image database of intracellular structures in Arabidopsis guard cells.2013

    • Author(s)
      Higaki T, Kustuna N, Hasezawa S
    • Journal Title

      BMC Plant Biology

      Volume: 13 Issue: 1 Pages: 81-81

    • DOI

      10.1186/1471-2229-13-81

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] スクロース水浸処理により誘導される気孔クラスターは孔辺細胞に特徴的な細胞内構造を有していた2016

    • Author(s)
      秋田佳恵,桧垣匠,馳澤盛一郎
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      岩手大学上田キャンパス(岩手県盛岡市)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] "ホンモンジゴケにおけるCopper-transporting P-type ATPaseの銅耐性能への貢献2016

    • Author(s)
      野村俊尚,井藤賀操,櫻井哲也,馳澤盛一郎,榊田均
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      岩手大学上田キャンパス(岩手県盛岡市)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] 葉表皮細胞における細胞壁湾曲のモデル解析2016

    • Author(s)
      桧垣匠,朽名夏麿,秋田佳恵,今村寿子,吉村賢二,馳澤盛一郎,三浦岳
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      岩手大学上田キャンパス(岩手県盛岡市)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] 糸状菌病原体うどんこ病菌の感染確立における宿主シロイヌナズナのアクチン脱重合因子ADF4の機能解析2015

    • Author(s)
      稲田のりこ,桧垣匠,馳澤盛一郎
    • Organizer
      日本植物学会第79回大会
    • Place of Presentation
      朱鷺メッセ:新潟コンベンションセンター(新潟県新潟市)
    • Year and Date
      2015-09-06
    • Related Report
      2015 Annual Research Report
  • [Presentation] Erwinia carotovora培養濾過液により誘導される液胞単純化の解析2015

    • Author(s)
      平川由美,桧垣匠,野村俊尚,馳澤盛一郎
    • Organizer
      日本植物学会第79回大会
    • Place of Presentation
      朱鷺メッセ:新潟コンベンションセンター(新潟県新潟市)
    • Year and Date
      2015-09-06
    • Related Report
      2015 Annual Research Report
  • [Presentation] スクロース水浸処理によりクラスター化した孔辺細胞における細胞内構造の検討2015

    • Author(s)
      秋田佳恵,桧垣匠,馳澤盛一郎
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学 世田谷キャンパス
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] タバコBY-2細胞を用いたErwinia carotovora培養濾過液誘導性細胞死における液胞単純化の観察2015

    • Author(s)
      平川由美,桧垣匠,野村俊尚,馳澤盛一郎
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学 世田谷キャンパス
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 植物細胞におけるゴルジ体形成・維持機構の解析2015

    • Author(s)
      伊藤容子,植村知博,湖城恵,馳澤盛一郎,上田貴志,中野明彦
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学 世田谷キャンパス
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 画像処理技術を用いたゴルジ体由来輝点の数および面積の定量的評価2015

    • Author(s)
      湖城恵,伊藤容子,中野明彦,馳澤盛一郎
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学 世田谷キャンパス
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] スクロース水溶液水浸処理により形成される気孔クラスターの解析2014

    • Author(s)
      秋田佳恵,桧垣匠,馳澤盛一郎
    • Organizer
      第78回日本植物学会
    • Place of Presentation
      明治大学 生田キャンパス
    • Year and Date
      2014-09-12 – 2014-09-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] バッチモード能動学習による顕微鏡画像の自動分類

    • Author(s)
      朽名夏麿,馳澤盛一郎
    • Organizer
      日本植物生理学会第55回年会
    • Place of Presentation
      富山大学五福キャンパス(富山県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] UV-BストレスがタバコBY-2培養細胞の増殖と生存率に与える影響

    • Author(s)
      高橋真哉,朽名夏麿,竹内祐一,馳澤盛一郎
    • Organizer
      日本植物生理学会第55回年会
    • Place of Presentation
      富山大学五福キャンパス(富山県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細胞質分裂の進行機構の解明

    • Author(s)
      村田隆,佐野敏夫,笹部美和子,野中茂紀,東山哲也,馳澤盛一郎,長谷部光泰
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      :北海道大学札幌キャンパス(北海道)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 能動学習を用いた画像分類法による細胞周期推定

    • Author(s)
      朽名夏麿,馳澤盛一郎
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      :北海道大学札幌キャンパス(北海道)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ショ糖水溶液による水浸処理はシロイヌナズナの葉におけるone-cell spacing ruleを破綻させた

    • Author(s)
      秋田佳恵,桧垣匠,馳澤盛一郎
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      :北海道大学札幌キャンパス(北海道)
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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