Study in the mechanism of differentiation and morphogenesis in plant cells by imaging quantitative analysis and simulation
| Project/Area Number |
25291056
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUNAGA Sachihiro 東京理科大学, 理工学部, 教授 (40323448)
TAMAOKI Masanori 国立研究開発法人国立環境研究所, 生物・生態系環境研究センター, 主任研究員 (00311324)
KUTSUNA Natsumaro 東京大学, 大学院新領域創成科学研究科, 特任准教授 (70578559)
TAKAHASHI Shinya 筑波大学, 北アフリカ研究センター, 副主任研究員 (80370419)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Keywords | オルガネラ / 細胞骨格 / 細胞壁 / イメージング / 画像定量解析 |
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| Outline of Final Research Achievements |
In this study, the intracellular structure-labeled transformed plants and imaging-quantitative analysis techniques have been developed. The cell morphogenesis and dynamics of the intracellular structures were analyzed by the image processing. The following four items were performed. (1) imaging-quantitative analysis of leaf epidermal cells, (2) analysis of artificial deformation and cell morphology in leaf epidermal cell wall, (3) analysis of the change in plant cell morphogenesis by high CO2 and UV-B treatments, (4) development of imaging-quantitative analysis techniques. Finally, a number of important knowledges, obtained for each item, have been published in many papers.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Accumulation of fluorescent proteins derived from a trans-Golgi cisternal membrane marker and paramural bodies in interdigitated apoplastic space in Arabidopsis leaf epidermis2016
Author(s)
Akita, K., Kobayashi, M., Sato, M., Kutsuna, N., Ueda, T., Toyooka, K., Nagata, N., Hasezawa, S., and Higaki, T.
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Journal Title
Protoplasma
Volume: in press
Issue: 1
Pages: 367-377
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] An atypical tubulin kinase mediates stress-induced microtubule depolymer ization in Arabidopsis2013
Author(s)
Satoshi Fujita, Jaromir Pytela, Takashi Hotta, Takehide Kato, Takahiro Hamada, Rie Akamatsu, Yasumasa Ishida, Natsumaro Kutsuna, Seiichiro Hasezawa, Yuko Nomura, Hirofumi Nakagami, Takashi Hashimoto
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Journal Title
Curr. Biol
Volume: 23
Issue: 20
Pages: 1969-1978
DOI
Related Report
Peer Reviewed
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[Journal Article] A Munc13-like protein in Arabidopsis mediates H+-ATPase translocation that is essential for stomatal responses2013
Author(s)
Hashimoto-Sugimoto, M., Higaki, T., Yaeno, T., Nagami, A., Irie, M., Fujimi M., Miyamoto, M., Akita, K., Negi, J., Shirasu, K., Hasezawa, S. and Iba, K.
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Journal Title
Nature Commun.
Volume: 4
Issue: 1
Pages: 2215-2215
DOI
Related Report
Peer Reviewed / Open Access
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