• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Interactions between the state transitions and phototaxis in the green alga Chlamydomonas

Research Project

Project/Area Number 25291058
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionTokyo Institute of Technology

Principal Investigator

Wakabayashi Ken-ichi  東京工業大学, 科学技術創成研究院, 准教授 (80420248)

Co-Investigator(Kenkyū-buntansha) 広野 雅文  法政大学, 生命科学部, 教授 (10212177)
Research Collaborator Hisabori Toru  東京工業大学, 科学技術創成研究院, 教授
Sugiura Kazunori  東京工業大学, 科学技術創成研究院, 博士研究員
Ueki Noriko  東京工業大学, 科学技術創成研究院, 博士研究員
Ide Takahiro  東京工業大学, 資源化学研究所, 博士研究員
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Keywords走光性 / 光合成 / レドックス / 鞭毛 / 光受容 / クラミドモナス / ステート遷移 / レドックス制御 / チオレドキシン / 真核生物鞭毛・繊毛
Outline of Final Research Achievements

Phototaxis is a cellular behavior in that cells move toward or against from the light source (positive or negative phototaxis, respectively). For phototrophic microorganisms, switching between positive and negative phototaxis is crucial to inhabit under suitable light intensity for photosynthesis. A unicellular green alga Chlamydomonas reinhardtii is a model organism for photo-behavioral responses. Previously, we found that cellular redox (reduction-oxidation) signal regulates this switching. In this research, to elucidate the sign-switching mechanism, we identified causative genes of mutants that show different sign of phototaxis. In parallel, we estimated intra flagellar redox potentials under different light conditions.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (41 results)

All 2017 2016 2015 2014 2013 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 3 results,  Acknowledgement Compliant: 3 results) Presentation (32 results) (of which Int'l Joint Research: 7 results,  Invited: 10 results) Remarks (3 results)

  • [Journal Article] 緑藻クラミドモナスの鞭毛運動と光行動2017

    • Author(s)
      若林憲一、中島昌子、井手隆広、植木紀子
    • Journal Title

      日本プランクトン学会報

      Volume: 64 Pages: 61-66

    • NAID

      130007609952

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of the agg1 mutation responsible for negative phototaxis in a "wild-type" strain of Chlamydomonas reinhardtii2016

    • Author(s)
      Ide, T., Mochiji, S., Ueki, N., Yamaguchi, K., Shigenobu, S., Hirono, M., Wakabayashi, K.
    • Journal Title

      Biochemistry and Biophysics Reports

      Volume: 7 Pages: 379-385

    • DOI

      10.1016/j.bbrep.2016.07.016

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Eyespot-dependent determination of the phototactic sign in Chlamydomonas reinhardtii2016

    • Author(s)
      Ueki, N., Ide, T., Mochiji, S., Kobayashi, Y., Tokutsu, R., Ohnishi, N., Yamaguchi, K., Shigenobu, S., Tanaka, K., Minagawa, J., Hisabori, T., Hirono, M., Wakabayashi, K.
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 印刷中 Issue: 19 Pages: 5266-5304

    • DOI

      10.1073/pnas.1525538113

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Cooperative binding of the outer arm docking complex underlies the regular arrangement of outer arm dynein in the axoneme.2014

    • Author(s)
      Mikito Owa, Akane Furuta, Jiro Usukura, Fumio Arisaka, Stephen M. King, George B. Witman, Ritsu Kamiya, Ken-ichi Wakabayashi
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 111(26) Issue: 26 Pages: 9455-9460

    • DOI

      10.1073/pnas.1403101111

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Protein-protein interactions between intermediate chains and the docking complex of Chlamydomonas flagellar outer arm dynein.2013

    • Author(s)
      Ide T, Owa M, King SM, Kamiya R, Wakabayashi K.
    • Journal Title

      FEBS Letter

      Volume: 587(14) Issue: 14 Pages: 2143-2149

    • DOI

      10.1016/j.febslet.2013.05.058

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Three-Dimensional Tracking of <i>Chlamydomonas</i> in Dark Field Microscopy2013

    • Author(s)
      荒井祐介,若林憲一,吉川雅英,奥寛雅,石川正俊
    • Journal Title

      Journal of the Robotics Society of Japan

      Volume: 31 Issue: 10 Pages: 1028-1035

    • DOI

      10.7210/jrsj.31.1028

    • NAID

      130003396218

    • ISSN
      0289-1824, 1884-7145
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] 緑藻の光行動における鞭毛運動調節:クラミドモナスとボルボックス2017

    • Author(s)
      植木紀子、若林憲一
    • Organizer
      第58回日本植物生理学会
    • Place of Presentation
      鹿児島・鹿児島大学群元キャンパス
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] クラミドモナスのγ-tubulin 優性突然変異 bld132017

    • Author(s)
      中澤友紀 、堀井麻央 、渡辺早紀 、大月萌子 、苗加彰 、若林憲一 、廣野雅文
    • Organizer
      生体運動研究合同班会議
    • Place of Presentation
      神戸・神戸国際会議場
    • Year and Date
      2017-01-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] クラミドモナスにおける走光性の正負と「利き鞭毛」の関係2017

    • Author(s)
      中島昌子 、植木紀子 、若林憲一
    • Organizer
      生体運動研究合同班会議
    • Place of Presentation
      神戸・神戸国際会議場
    • Year and Date
      2017-01-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] ボルボックスのカルシウム依存的繊毛打逆転と走光性2017

    • Author(s)
      植木紀子、若林憲一
    • Organizer
      生体運動研究合同班会議
    • Place of Presentation
      神戸・神戸国際会議場
    • Year and Date
      2017-01-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] Photo-behavior in green algae: how light modulates flagellar motility2017

    • Author(s)
      Masako Nakajima, Noriko Ueki, Ken-ichi Wakabayashi
    • Organizer
      光合成科学:エネルギーとバイオマス
    • Place of Presentation
      横浜・東工大すずかけホール
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Modulation of flagellar motility during phototactic turning in Chlamydomonas2016

    • Author(s)
      Masako Nakajima, Noriko Ueki, Ken-ichi Wakabayashi
    • Organizer
      The 28th CDB Meeting: Cilia and Centrosomes: Current Advances and Future Directions
    • Place of Presentation
      神戸・理研CDB
    • Year and Date
      2016-11-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The molecular toolbox for building axonemal microtubules2016

    • Author(s)
      Mikito Owa, Takayuki Uchihashi, Haruaki Yanagisawa, Takashi Yamano, Hiro Iguchi, Hideya Fukuzawa, Ken-ichi Wakabayashi, Toshio Ando, Masahide Kikkawa
    • Organizer
      The 28th CDB Meeting: Cilia and Centrosomes: Current Advances and Future Directions
    • Place of Presentation
      神戸・理研CDB
    • Year and Date
      2016-11-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 緑藻クラミドモナスの走光性発現メカニズム2016

    • Author(s)
      若林憲一
    • Organizer
      遺伝研研究会「水圏微生物のエネルギー利用戦略とその相互作用に関する研究会」
    • Place of Presentation
      三島・遺伝研
    • Year and Date
      2016-11-03
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Visualization and quantification of the redox potential in the Chlamydomonas flagella2016

    • Author(s)
      Yuta Nishimaki, Kazunori Sugiura, Toru Hisabori, Ken-ichi Wakabayashi
    • Organizer
      The 17th International Conference on the Cell and Molecular Biology of Chlamydomonas
    • Place of Presentation
      京都・京都国際会館
    • Year and Date
      2016-06-26
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Eyespot-dependent determination of the phototactic sign in Chlamydomonas reinhardtii2016

    • Author(s)
      Noriko Ueki, Takahiro Ide, Shota Mochiji, Yuki Kobayashi, Ryutaro Tokutsu, Norikazu Ohnishi, Katushi Yamaguchi, Shuji Shigenobu, Kan Tanaka, Jun Minagawa, Toru Hisabori, Masafumi Hirono, Ken-ichi Wakabayashi
    • Organizer
      The 17th International Conference on the Cell and Molecular Biology of Chlamydomonas
    • Place of Presentation
      京都・京都国際会館
    • Year and Date
      2016-06-26
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Isolation of a non-phototactic Chlamydomonas mutant that shows alternate flagellar beatings2016

    • Author(s)
      Akinori Koitabashi, Masako Nakajima, Noriko Ueki, Kogiku Shiba, Takashi Ymano, Hideya Fukuzawa, Inaba Kazuo, Toru Hisabori, Ken-ichi Wakabayashi
    • Organizer
      The 17th International Conference on the Cell and Molecular Biology of Chlamydomonas
    • Place of Presentation
      京都・京都国際会館
    • Year and Date
      2016-06-26
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification of the agg1 mutation responsible for negative phototaxis in a "wild-type" strain of Chlamydomonas reinhardtii2016

    • Author(s)
      Takahiro Ide, Shota Mochuji, Noriko Ueki, Katsushi Yamaguchi, Shuji Shigenobu, Masafumi Hirono, Ken-ichi Wakabayashi
    • Organizer
      The 17th International Conference on the Cell and Molecular Biology of Chlamydomonas
    • Place of Presentation
      京都・京都国際会館
    • Year and Date
      2016-06-26
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 緑藻クラミドモナスとボルボックスの走光性のしくみ2016

    • Author(s)
      若林憲一
    • Organizer
      第34回エアロアクアバイオメカニズム学会定例講演会
    • Place of Presentation
      東京・東京工業大学大岡山キャンパス
    • Year and Date
      2016-03-23
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] クラミドモナス走光性における細胞レンズ効果の影響と眼点の役割2016

    • Author(s)
      植木紀子、井手 隆広、小林勇気、田中 寛、久堀 徹、若林 憲一
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      盛岡・岩手大学上田キャンパス
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] 緑藻クラミドモナスの走光性メカニズム - 光受容と鞭毛運動の連関 -2016

    • Author(s)
      若林憲一
    • Organizer
      日本プランクトン学会春季シンポジウム「光環境を巡る植物プランクトンの生理生態学的最前線
    • Place of Presentation
      東京・東京大学本郷キャンパス
    • Year and Date
      2016-03-14
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Calcium-dependent photobehavior of the unicellular green alga Chlamydomonas reinhardtii2015

    • Author(s)
      Ken-ichi Wakabayashi
    • Organizer
      第38回日本分子生物学会年会・第88回日本生化学会大会合同大会
    • Place of Presentation
      神戸・ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] クラミドモナス走光性符号決定における眼点の役割2015

    • Author(s)
      植木紀子、持地翔太 、井手隆広 、山口勝司 、重信秀治 、得津隆太郎 、大 西紀和、皆川純、廣野雅文、若林憲一
    • Organizer
      日本植物生理学会第56回年会
    • Place of Presentation
      東京農業大学(東京都)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] クラミドモナス走光性符号決定における眼点の役割2015

    • Author(s)
      植木紀子, 持地翔太, 井手隆広, 廣野雅文, 若林憲一
    • Organizer
      生体運動研究合同班会議
    • Place of Presentation
      学習院大学(東京都)
    • Year and Date
      2015-01-07 – 2015-01-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] クラミドモナス走光性符号決定における眼点の役割2014

    • Author(s)
      若林憲一 、持地翔太 、植木紀子 、井手隆広 、山口勝司 、重信秀治 、得津隆太郎 、大 西紀和、皆川純、廣野雅文
    • Organizer
      第11回クラミドモナス研究会
    • Place of Presentation
      かるぽーと(高知県)
    • Year and Date
      2014-10-03 – 2014-10-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] 緑藻ボルボックス目における光反応行動調節機構2014

    • Author(s)
      植木紀子、箕浦高子、若林憲一
    • Organizer
      第11回クラミドモナス研究会
    • Place of Presentation
      かるぽーと(高知県)
    • Year and Date
      2014-10-03 – 2014-10-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] Sign-Control of Chlamydomonas Phototaxis by Redox Poise2014

    • Author(s)
      Ken-ichi Wakabayashi
    • Organizer
      16th International Congress on Photobiology
    • Place of Presentation
      コルドバ国立大学(アルゼンチン)
    • Year and Date
      2014-09-08 – 2014-09-12
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] クラミドモナス走光性符号異常ミュータントの解析2014

    • Author(s)
      持地翔太、井手隆広、山口勝司、重信秀治、神谷律、廣野雅文、若林憲一
    • Organizer
      生体運動研究合同班会議
    • Place of Presentation
      千葉(千葉大学)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Regulation of flagellar beating studied using the unicellular green alga Chlamydomonas reinhardtii2014

    • Author(s)
      Ken-ichi Wakabayashi
    • Organizer
      The 17th SANKEN international symposium joined with the 2nd international symposium of nano-macro materials, devices, and system research alliance project
    • Place of Presentation
      吹田(大阪大学)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 緑藻クラミドモナスの走光性符号調節2013

    • Author(s)
      若林憲一
    • Organizer
      第3回微細藻類研究会
    • Place of Presentation
      岡崎(岡崎カンファレンスセンター)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Analysis of Protein-Protein Interactions Required for the Assembly of Outer Arm Dyneins in Chlamydomonas Axonemes2013

    • Author(s)
      Takahiro IDE, Mikito Owa, Kaoru Yoshida, Manabu Yoshida, Stephen M. King, Ritsu Kamiya, and Ken-ichi Wakabayashi
    • Organizer
      FASEB 2013 Conference on the Biology of Cilia and Flagella
    • Place of Presentation
      Niagara Falls, NY, USA (Sheraton Niagara)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Cooperative binding of the outer dynein arm docking complex (ODA-DC) on the flagellar axoneme in Chlamydomonas2013

    • Author(s)
      Mikito Owa, Takahiro Ide, Stephen M. King, Ritsu Kamiya, and Ken-ichi Wakabayashi
    • Organizer
      FASEB 2013 Conference on the Biology of Cilia and Flagella
    • Place of Presentation
      Niagara Falls, NY, USA (Sheraton Niagara)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Redox Regulation of Cell Motility in the Green Alga Chlamydomonas reinhardtii.2013

    • Author(s)
      Ken-ichi Wakabayashi
    • Organizer
      The 25the CDB meeting “Cilia and Centrosomes: from Fertilization to Cancer”
    • Place of Presentation
      神戸(理研CDB)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Structural and Biochemical Properties of the Outer-Dynein-Arm Docking Complex (ODA-DC) in Chlamydomonas flagella2013

    • Author(s)
      Mikito Owa, Takahiro Ide, Stephen M. King, Ritsu Kamiya, and Ken-ichi Wakabayashi
    • Organizer
      The 25the CDB meeting “Cilia and Centrosomes: from Fertilization to Cancer”
    • Place of Presentation
      神戸(理研CDB)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Analysis of Protein-Protein Interactions Required for the Assembly of Outer Arm Dyneins in Chlamydomonas Axonemes2013

    • Author(s)
      Takahiro IDE, Mikito Owa, Kaoru Yoshida, Manabu Yoshida, Stephen M. King, Ritsu Kamiya, and Ken-ichi Wakabayashi
    • Organizer
      The 25the CDB meeting “Cilia and Centrosomes: from Fertilization to Cancer”
    • Place of Presentation
      神戸(理研CDB)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Structure and properties of the Outer Dynein Arm Docking Complex in Chlamydomonas2013

    • Author(s)
      Ken-ichi Wakabayashi
    • Organizer
      Dynein2013 International Workshop
    • Place of Presentation
      神戸(Orbis Hall)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Analysis of Protein-Protein Interactions Required for the Assembly of Outer Arm Dyneins in Chlamydomonas Axonemes2013

    • Author(s)
      Takahiro IDE, Mikito Owa, Kaoru Yoshida, Manabu Yoshida, Stephen M. King, Ritsu Kamiya, and Ken-ichi Wakabayashi
    • Organizer
      Dynein2013 International Workshop
    • Place of Presentation
      神戸(Orbis Hall)
    • Related Report
      2013 Annual Research Report
  • [Presentation] クラミドモナス走光性符号異常ミュータントの次世代シークエンスによる解析2013

    • Author(s)
      持地翔太、井手隆広、山口勝司、重信秀治、神谷律、廣野雅文、若林憲一
    • Organizer
      第10回クラミドモナス研究会
    • Place of Presentation
      岡崎(基礎生物学研究所)
    • Related Report
      2013 Annual Research Report
  • [Remarks] 久堀・若林研ウェブサイト

    • URL

      http://www.res.titech.ac.jp/~junkan/Hisabori_HomePage/index.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 久堀・若林研究室ウェブサイト

    • URL

      http://www.res.titech.ac.jp/~junkan/Hisabori_HomePage/index.html

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
  • [Remarks] 東工大資源研 久堀・若林研究室のウェブサイト

    • URL

      http://www.res.titech.ac.jp/~junkan/Hisabori_HomePage/index.html

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2013-05-21   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi