• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Functional analysis of the nuclear-encoded pentatricopeptide repeat proteins involved in regulation of plant organellar gene expression

Research Project

Project/Area Number 25291059
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionNagoya University

Principal Investigator

Sugita Mamoru  名古屋大学, 遺伝子実験施設, 教授 (70154474)

Co-Investigator(Renkei-kenkyūsha) NAKAMURA Takahiro  九州大学, 農学研究院, 准教授 (10464398)
ICHINOSE Mizuho  名古屋大学, トランスフォーマティブ生命分子研究所, 特任助教 (60755718)
Research Collaborator SUGITA Chieko  名古屋大学, 遺伝子実験施設, 研究員 (30402457)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2015: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Keywords葉緑体 / ミトコンドリア / 転写後制御 / PPRタンパク質 / RNA結合認識 / RNAスプライシング / RNA編集 / ヒメツリガネゴケ / 遺伝子発現 / グループⅡイントロン / RNA結合配列 / RNase P / DYWドメイン
Outline of Final Research Achievements

Plant mitochondria and chloroplasts are the important organelles that are essential for plant growth. Both organelles possess their own gene expression system and their gene expression is tightly regulated at the post transcriptional level. However, protein factors involved in post transcriptional regulation are unknown. In this study, we identified novel pentatricopeptide repeat RNA-binding proteins required for tRNA 5’-end processing, RNA editing, and RNA splicing, using a model plant, the moss Physcomitrella patens.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (43 results)

All 2016 2015 2014 2013 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Acknowledgement Compliant: 4 results,  Open Access: 1 results) Presentation (25 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Book (4 results) Remarks (6 results)

  • [Journal Article] Diversity of plant circadian clocks: insights from studies of Chlamydomonas reinhardtii and Physcomitrella patens.2016

    • Author(s)
      2.Ryo, T., Matsuo, T., Yamashino, T., Ichinose, M., Sugita, M. and Aoki, S.
    • Journal Title

      Plant Signaling & Behavior

      Volume: 11 Issue: 1 Pages: e1116661-e1116661

    • DOI

      10.1080/15592324.2015.1116661

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plastid genome sequences of Gymnochlora stellata, Lotharella vacuolata, and Partenskyella glossopodia reveal remarkable structural conservation among chlorarachniophyte species.2016

    • Author(s)
      Suzuki S, Hirakawa Y, Kofuji R, Sugita M, Ishida K.
    • Journal Title

      Journal of Plant Research

      Volume: 印刷中 Issue: 4 Pages: 581-590

    • DOI

      10.1007/s10265-016-0804-5

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] P-class pentatricopeptide repeat protein PTSF1 is required for splicing of the plastid pre-tRNA(Ile) in Physcomitrella patens.2016

    • Author(s)
      Goto, S., Kawaguchi, Y., Sugita, C., Ichinose, M. and Sugita, M.
    • Journal Title

      The Plant Journal

      Volume: 87 Issue: 6 Pages: 493-503

    • DOI

      10.1111/tpj.13184

    • NAID

      120005893269

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Molecular characterization of three PRORP proteins in the moss Physcomitrella patens: nuclear PRORP protein is not essential for moss viability.2014

    • Author(s)
      Sugita, C., Komura, Y., Tanaka, K., Kometani, K., Satoh, H. and Sugita, M.
    • Journal Title

      PLoS ONE

      Volume: 9 Issue: 10 Pages: e108962-e108962

    • DOI

      10.1371/journal.pone.0108962

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification of a pentatricopeptide repeat RNA editing factor in Physcomitrella patens chloroplasts2014

    • Author(s)
      Mizuho Ichinose, Masato Uchida, Mamoru Sugita
    • Journal Title

      FEBS Letters

      Volume: 588 Issue: 21 Pages: 4060-4064

    • DOI

      10.1016/j.febslet.2014.09.031

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] DipM is required for peptidoglycan hydrolysis during chloroplast division.2014

    • Author(s)
      Miyagishima SY, Kabeya Y, Sugita C, Sugita M, Fujiwara T.
    • Journal Title

      BMC Plant Biol.

      Volume: 14 Issue: 1 Pages: 57-57

    • DOI

      10.1186/1471-2229-14-57

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Architecture of the PPR gene family in the moss Physcomitrella patens2013

    • Author(s)
      Mamoru Sugita, Mizuho Ichinose, Mizuki Ide, Chieko Sugita
    • Journal Title

      RNA Biology

      Volume: 10 Issue: 9 Pages: 1439-1445

    • DOI

      10.4161/rna.24772

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Two DYW subclass PPR proteins are involved in RNA editing of ccmFc and atp9 transcripts in the moss Physcomitrella patens : first complete set of PPR editing factors in plant mitochondria2013

    • Author(s)
      Mizuho Ichinose, Chieko Sugita, Yusuke Yagi, Takahiro Nakamura, Mamoru Sugita
    • Journal Title

      Plant Cell Physiology

      Volume: 54 Issue: 11 Pages: 1907-1916

    • DOI

      10.1093/pcp/pct132

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] クラミドモナスとゼニゴケのタンパク質性RNase Pの解析2016

    • Author(s)
      杉田千恵子、松尾拓哉、西浜竜一、河内孝之、杉田護
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      岩手大学上田キャンパス
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] ヒメツリガネゴケ葉緑体のpre-tRNAのスプライシングに関与するPPRタンパク質2016

    • Author(s)
      後藤誠也、一瀬瑞穂、杉田千恵子、杉田護
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      岩手大学上田キャンパス
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] ヒメツリガネゴケPPRタンパク質のRNA塩基認識の解析2016

    • Author(s)
      松田拓也、一瀬瑞穂、杉田護
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      岩手大学上田キャンパス
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] Functional analysis of P-type PPR proteins in the moss Physcomitrella patens2016

    • Author(s)
      Goto, S., Kawaguchi, Y., Ichinose, M., Sugita, C., Sugita, M.
    • Organizer
      IGER Annual meeting 2015
    • Place of Presentation
      Toyada Auditorium, Nagoya University
    • Year and Date
      2016-01-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] ヒメツリガネゴケのRRMを持つ葉緑体RNA結合タンパク質の機能解析2015

    • Author(s)
      内山博道、一瀬瑞穂、杉田千恵子、杉田護
    • Organizer
      日本植物学会第79回大会
    • Place of Presentation
      朱鷺メッセ:新潟コンベンションセンター
    • Year and Date
      2015-09-06
    • Related Report
      2015 Annual Research Report
  • [Presentation] ヒメツリガネゴケの葉緑体tRNAのスプライシングに関与する新規PPRタンパク質の同定2015

    • Author(s)
      後藤誠也、一瀬瑞穂、杉田千恵子、杉田護
    • Organizer
      日本植物学会第79回大会
    • Place of Presentation
      朱鷺メッセ:新潟コンベンションセンター
    • Year and Date
      2015-09-06
    • Related Report
      2015 Annual Research Report
  • [Presentation] A PLS-type PPR protein is involved in RNA splicing of nad5 pre-mRNA in the moss Physcomitrella patens.2015

    • Author(s)
      Nakajima, K., Kawaguchi, Y., Ichinose, M., Sugita, C., Sugita, M.
    • Organizer
      9th International Conference of Plant Mitochondrial Biology
    • Place of Presentation
      Wroclaw, Poland
    • Year and Date
      2015-05-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 植物オルガネラRNA編集におけるDYWドメインの機能解析2015

    • Author(s)
      一瀬瑞穂、杉田 護
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学世田谷キャンパス
    • Year and Date
      2015-03-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] パーティクルガンを用いたトランジェントアッセイによるヒメツリガネゴケの生物時計遺伝子の発現解析2015

    • Author(s)
      龍 昌志、一瀬瑞穂、樋口海杜、杉田 護、青木摂之
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学世田谷キャンパス
    • Year and Date
      2015-03-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] ゼニゴケのタンパク質性RNase Pの解析2015

    • Author(s)
      杉田千恵子、西浜竜一、河内孝之、杉田護
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学世田谷キャンパス
    • Year and Date
      2015-03-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒメツリガネゴケのP-type PPRタンパク質の機能解析(2)2015

    • Author(s)
      後藤誠也、一瀬瑞穂、加藤歩美、杉田千恵子、杉田 護
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学世田谷キャンパス
    • Year and Date
      2015-03-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] 植物オルガネラのPPRタンパク質とRNA編集2015

    • Author(s)
      一瀬瑞穂、杉田 護
    • Organizer
      第17回オルガネラワークショプ「オルガネラ機能の最適化メカニズム」
    • Place of Presentation
      東京大学駒場キャンパス21KOMCEE WEST B1階レクチャーホール
    • Year and Date
      2015-03-15
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] RNA編集におけるPPRタンパク質のDYWドメインの役割2015

    • Author(s)
      一瀬瑞穂、杉田 護
    • Organizer
      第4回植物RNA研究者ネットワークシンポジウム
    • Place of Presentation
      京都大学理学部セミナーハウス
    • Year and Date
      2015-01-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] オルガネラRNA編集におけるDYWドメインの役割2014

    • Author(s)
      一瀬瑞穂、杉田 護
    • Organizer
      日本植物学会第78回大会
    • Place of Presentation
      明治大学生田キャンパス
    • Year and Date
      2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒメツリガネゴケのミトコンドリアのRNAスプライシングに働く新規のPLS-type PPRタンパク質の同定2014

    • Author(s)
      川口康弘、一瀬瑞穂、中島健策、杉田千恵子、杉田 護
    • Organizer
      日本植物学会第78回大会
    • Place of Presentation
      明治大学生田キャンパス
    • Year and Date
      2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒメツリガネゴケの遺伝子ターゲティングによるPPR遺伝子ファミリーの機能解析2014

    • Author(s)
      一瀬瑞穂、杉田 護
    • Organizer
      日本蘚苔類学会第43回大会(青森大会)
    • Place of Presentation
      青森県十和田市星野リゾート奥入瀬渓流ホテル
    • Year and Date
      2014-08-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒメツリガネゴケの3種類のタンパク質性RNase Pの解析2014

    • Author(s)
      杉田千恵子,田中惟睦,米谷一樹,香村吉洋,杉田 護
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学五福キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] ヒメツリガネゴケの葉緑体RNA 編集因子の同定2014

    • Author(s)
      一瀬瑞穂,内田雅人,杉田 護
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学五福キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] ヒメツリガネゴケの2 つのオルガネラ局在タンパク質性RNasePの解析2014

    • Author(s)
      田中惟睦,杉田千恵子,香村吉洋,杉田 護
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学五福キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] ヒメツリガネゴケのP-type PPRタンパク質の機能解析2014

    • Author(s)
      川口康弘,一瀬瑞穂,杉田千恵子,杉田 護
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学五福キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] 葉緑体局在PPRタンパク質がコケ原糸体細胞の分化に関与する2013

    • Author(s)
      後藤誠也、一瀬瑞穂、杉田千恵子、杉田 護
    • Organizer
      第4回日本光合成学会年会およびシンポジウム
    • Place of Presentation
      名古屋大学野依記念学術交流館
    • Related Report
      2013 Annual Research Report
  • [Presentation] Mamoru Sugita, Mizuho Ichinose, Chieko Sugita, Korechika Tanaka, Yasuhiro Kawaguchi, and Seiya Goto, Functional analysis of the pentatricopeptide repeat protein family in Physcomitrella patens.2013

    • Author(s)
      Mamoru Sugita, Mizuho Ichinose, Chieko Sugita, Korechika Tanaka, Yasuhiro Kawaguchi, and Seiya Goto
    • Organizer
      16th International Conference MOSS 2013
    • Place of Presentation
      Masaryk Center, Prague, Czech Republic
    • Related Report
      2013 Annual Research Report
  • [Presentation] 葉緑体局在PPRタンパク質がヒメツリガネゴケの原糸体細胞の分化に関与する2013

    • Author(s)
      後藤誠也、一瀬瑞穂、杉田千恵子、杉田 護
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      北海道大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] ヒメツリガネゴケのミトコンドリアccmFc mRNAの近接した2カ所のRNA編集部位に働くDYWサブクラスPPRタンパク質2013

    • Author(s)
      一瀬瑞穂、杉田千恵子、八木祐介、中村崇裕、杉田 護
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      北海道大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Pentatricopeptide repeat (PPR) proteins in Physcomitrella patens2013

    • Author(s)
      Mizuho Ichinose, Chieko Sugita, Korechika Tanaka, Yasuhiro Kawaguchi, Seiya Goto, Akimi Takahashi and Mamoru Sugita
    • Organizer
      CREST symposium, Regulation of photosynthesis and chloroplast function
    • Place of Presentation
      Kyoto University
    • Related Report
      2013 Annual Research Report
  • [Book] Web版光合成事典2015

    • Author(s)
      杉田 護、他115名
    • Publisher
      日本光合成学会
    • Related Report
      2014 Annual Research Report
  • [Book] 「光合成生物の進化と生命科学」(三村徹郎、川井浩史編)2014

    • Author(s)
      杉田 護、他11名
    • Total Pages
      194
    • Publisher
      培風館
    • Related Report
      2014 Annual Research Report
  • [Book] Chloroplast Biotechnology: Methods and Protocols (Pal Maliga, ed.), Methods in Molecular Biology, Vol. 132, Springer Protocols2014

    • Author(s)
      Sugita, M.
    • Total Pages
      542
    • Publisher
      Humana Press
    • Related Report
      2013 Annual Research Report
  • [Book] Plastid Development in Leaves during Growth and Senescence (eds. Basanti Biswal, Karin Krupinska and Udaya C. Biswal), Advances in Photosynthesis and Respiration 362013

    • Author(s)
      Kanamaru, K. and Sugita, M.
    • Total Pages
      685
    • Publisher
      Springer
    • Related Report
      2013 Annual Research Report
  • [Remarks] 名古屋大学大学院理学研究科生命理学専攻ホームページ、論文紹介

    • URL

      http://www.bio.nagoya-u.ac.jp/paper/index.html

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
  • [Remarks] 名古屋大学遺伝子実験施設・杉田研究室ホームページ、論文リスト

    • URL

      http://www.gene.nagoya-u.ac.jp/~sugita-g/ronbun.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 杉田研究室ホームページ論文リスト

    • URL

      http://www.gene.nagoya-u.ac.jp/~sugita-g/ronbun.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] Web版光合成事典

    • URL

      http://photosyn.jp/pwiki/index.php?FrontPage

    • Related Report
      2014 Annual Research Report
  • [Remarks] 杉田研究室ホームページ、論文リスト

    • URL

      http://www.gene.nagoya-u.ac.jp/~sugita-g/ronbun.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] 名古屋大学大学院理学研究科生命理学専攻ホームページ、論文紹介

    • URL

      http://www.bio.nagoya-u.ac.jp/paper/index.html

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2013-05-21   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi