Functional analysis of the nuclear-encoded pentatricopeptide repeat proteins involved in regulation of plant organellar gene expression
Project/Area Number |
25291059
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Plant molecular biology/Plant physiology
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Research Institution | Nagoya University |
Principal Investigator |
Sugita Mamoru 名古屋大学, 遺伝子実験施設, 教授 (70154474)
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Co-Investigator(Renkei-kenkyūsha) |
NAKAMURA Takahiro 九州大学, 農学研究院, 准教授 (10464398)
ICHINOSE Mizuho 名古屋大学, トランスフォーマティブ生命分子研究所, 特任助教 (60755718)
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Research Collaborator |
SUGITA Chieko 名古屋大学, 遺伝子実験施設, 研究員 (30402457)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2015: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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Keywords | 葉緑体 / ミトコンドリア / 転写後制御 / PPRタンパク質 / RNA結合認識 / RNAスプライシング / RNA編集 / ヒメツリガネゴケ / 遺伝子発現 / グループⅡイントロン / RNA結合配列 / RNase P / DYWドメイン |
Outline of Final Research Achievements |
Plant mitochondria and chloroplasts are the important organelles that are essential for plant growth. Both organelles possess their own gene expression system and their gene expression is tightly regulated at the post transcriptional level. However, protein factors involved in post transcriptional regulation are unknown. In this study, we identified novel pentatricopeptide repeat RNA-binding proteins required for tRNA 5’-end processing, RNA editing, and RNA splicing, using a model plant, the moss Physcomitrella patens.
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Report
(4 results)
Research Products
(43 results)
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[Journal Article] Diversity of plant circadian clocks: insights from studies of Chlamydomonas reinhardtii and Physcomitrella patens.2016
Author(s)
2.Ryo, T., Matsuo, T., Yamashino, T., Ichinose, M., Sugita, M. and Aoki, S.
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Journal Title
Plant Signaling & Behavior
Volume: 11
Issue: 1
Pages: e1116661-e1116661
DOI
Related Report
Peer Reviewed
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[Journal Article] Molecular characterization of three PRORP proteins in the moss Physcomitrella patens: nuclear PRORP protein is not essential for moss viability.2014
Author(s)
Sugita, C., Komura, Y., Tanaka, K., Kometani, K., Satoh, H. and Sugita, M.
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Journal Title
PLoS ONE
Volume: 9
Issue: 10
Pages: e108962-e108962
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Functional analysis of P-type PPR proteins in the moss Physcomitrella patens2016
Author(s)
Goto, S., Kawaguchi, Y., Ichinose, M., Sugita, C., Sugita, M.
Organizer
IGER Annual meeting 2015
Place of Presentation
Toyada Auditorium, Nagoya University
Year and Date
2016-01-08
Related Report
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[Presentation] A PLS-type PPR protein is involved in RNA splicing of nad5 pre-mRNA in the moss Physcomitrella patens.2015
Author(s)
Nakajima, K., Kawaguchi, Y., Ichinose, M., Sugita, C., Sugita, M.
Organizer
9th International Conference of Plant Mitochondrial Biology
Place of Presentation
Wroclaw, Poland
Year and Date
2015-05-17
Related Report
Int'l Joint Research
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[Presentation] Mamoru Sugita, Mizuho Ichinose, Chieko Sugita, Korechika Tanaka, Yasuhiro Kawaguchi, and Seiya Goto, Functional analysis of the pentatricopeptide repeat protein family in Physcomitrella patens.2013
Author(s)
Mamoru Sugita, Mizuho Ichinose, Chieko Sugita, Korechika Tanaka, Yasuhiro Kawaguchi, and Seiya Goto
Organizer
16th International Conference MOSS 2013
Place of Presentation
Masaryk Center, Prague, Czech Republic
Related Report
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