Active growth repression of plants under environmental stresses

• Project/Area Number: 25291060
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Osaka University
• Principal Investigator: Kakimoto Tatsuo 大阪大学, 理学(系)研究科(研究院), 教授 (70214260)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2015: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2013: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
• Keywords: 植物 / 成長 / 環境 / ストレス / 情報伝達 / シロイヌナズナ / 環境応答 / 突然変異体 / アブシジン酸 / 浸透圧

Outline of Final Research Achievements

Plant growth is retarded under biotic or abiotic stresses. Low environmental water potential generally represses plant growth, especially the growth of shoots. The growth reduction is in part simply because plants cannot grow well, but also in part considered to be an active adaptive response of plants. We focused on stress responses of growth of leaf epidermal cells, and uncovered the molecular mechanisms of growth repression under high osmolarity. The leaf epidermal stem cells (MMCs) is maintained by the transcription factor SPEECHLESS (SPCH). Here we found that osmotic stress activates the MAPK cascade, which in turn phosphorylates SPCH and leads to decrease in SPCH protein level. Blocking the MAPK activity inhibited the decrease in MMC number and the growth reduction caused by osmotic stress. These results highlighted the integration of stress-induced signaling and internal developmental signaling.

Research Products

• [Int'l Joint Research] 精華大学(中国)
• [Int'l Joint Research] ケンブリッジ大学(英国)
• [Journal Article] 植物ホルモン研究の発展 (2016)
  • Author(s): 柿本辰男
  • Journal Title: 遺伝 Volume: 70 Pages: 348-349
• [Journal Article] Cytokinin is required for escape but not release from auxin mediated apical dominance. (2015)
  • Author(s): Muller D, Waldie T, Miyawaki K, To JP, Melnyk CW, Kieber JJ, Kakimoto T, Leyser O.
  • Journal Title: Plant J Volume: 82 Issue: 5 Pages: 874-886
  • DOI: 10.1111/tpj.12862
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Distinct Characteristics of Indole-3-Acetic Acid and Phenylacetic Acid, Two Common Auxins in Plants. (2015)
  • Author(s): Sugawara, S., Mashiguchi, K., Tanaka, K., Hishiyama, S., Sakai, T., Hanada, K., Kinoshita-Tsujimura, K., Yu, H., Dai, X., Takebayashi, Y., Takeda-Kamiya, N., Kakimoto, T., Kawaide, H., Natsume, M., Estelle, M., Zhao, Y., *Hayashi, K., Kamiya, Y.,Kasahara, H.
  • Journal Title: Plant Cell Physiol Volume: 56 Issue: 8 Pages: 1641-1654
  • DOI: 10.1093/pcp/pcv088
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] AINTEGUMENTA and the D-type cyclin CYCD3;1 regulate root secondary growth and respond to cytokinins. (2015)
  • Author(s): Randall RS, Miyashima S, Blomster T, Zhang J, Elo A, Karlberg A, Immanen J, Nieminen K, Lee JY, Kakimoto T, Blajecka K, Melnyk CW, Alcasabas A, Forzani C, Matsumoto-Kitano M, Mähönen AP, Bhalerao R, Dewitte W, Helariutta Y, Murray JA.
  • Journal Title: Biol Open. Volume: 4 Issue: 10 Pages: 1229-1236
  • DOI: 10.1242/bio.013128
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A parasitic nematode releases cytokinin that controls cell division and orchestrates feeding site formation in host plants. (2015)
  • Author(s): Siddique S, Radakovic ZS, De La Torre CM, Chronis D, Novak O, Ramireddy E, Holbein J, Matera C, Hutten M, Gutbrod P, Anjam MS, Rozanska E, Habash S, Elashry A, Sobczak M, Kakimoto T, Strnad M, Schmulling T, Mitchum MG, Grundler FM.
  • Journal Title: Proc Natl Acad Sci U S A Volume: 112 Issue: 41 Pages: 12669-12674
  • DOI: 10.1073/pnas.1503657112
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Auxin sensitivities of all Arabidopsis Aux/IAAs for degradation in the presence of every TIR1/AFB. (2015)
  • Author(s): Shimizu-Mitao Y, Kakimoto T.
  • Journal Title: Plant Cell Physiology Volume: 55 Issue: 8 Pages: 1450-1459
  • DOI: 10.1093/pcp/pcu077
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Pathways involved in vanadate-induced root hair formation in Arabidopsis. (2014)
  • Author(s): Lin CY, Huang LY, Chi WC, Huang TL, Kakimoto T, Tsai CR, Huang HJ.
  • Journal Title: Physiol Plant. Volume: 153 Issue: 1 Pages: 137-148
  • DOI: 10.1111/ppl.12229
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Arabidopsis reduces growth under osmotic stress by decreasing SPEECHLESS protein. (2014)
  • Author(s): Kumari A, Jewaria PK, Bergmann DC, Kakimoto T.
  • Journal Title: Plant Cell Physiology Volume: 55 Issue: 12 Pages: 2037-2046
  • DOI: 10.1093/pcp/pcu159
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] BEX1/ARF1A1C is Required for BFA—Sensitive Recycling of PIN Auxin Tran sporters and Auxin—Mediated Development in Arabidopsis. (2014)
  • Author(s): Tanaka H, Nodzylski T, Kitakura S, Feraru MI, Sasabe M, Ishikawa T, Kleine-Vehn J, Kakimoto T, Fr iml J.
  • Journal Title: Plant Cell Physiol. Volume: 55 Issue: 4 Pages: 737-749
  • DOI: 10.1093/pcp/pct196
  • Peer Reviewed
• [Journal Article] EPIDERMAL PATTERNING FACTOR LIKE5 peptide represses stomatal development by inhibiting meristemoid maintenance in Arabidopsis thaliana. (2013)
  • Author(s): Niwa T, Kondo T, Nishizawa M, Kajita R, Kakimoto T, Ishiguro S.
  • Journal Title: Biosci Biotechnol Biochem. Volume: 77 Pages: 1287-1295
  • NAID: 10031184696
  • Peer Reviewed
• [Journal Article] Cell polarity and patterning by PIN trafficking through early endosomal compartments in Arabidopsis thaliana. (2013)
  • Author(s): Tanaka H, Kitakura S, Rakusová H , Uemura T, Feraru MI, De Rycke R, Robert S, Kakimoto T, Friml J.
  • Journal Title: PLoS Genet. Volume: 9 Issue: 5 Pages: e1003540-e1003540
  • DOI: 10.1371/journal.pgen.1003540
  • Peer Reviewed
• [Journal Article] Differential Effects of Peptides Stomagen, EPF1, and EPF2 on Activation of the MAP Kinase MPK6 and the SPCH Protein Level. (2013)
  • Author(s): Jewaria PK, Hara T, Tanaka H, Ko ndo T, Betsuyaku S, Sawa S, Sakagami Y, Aimoto S, Kakimoto T.
  • Journal Title: Plant Cell Physiol Volume: 59 Issue: 8 Pages: 1253-1262
  • DOI: 10.1093/pcp/pct076
  • NAID: 10031195179
  • Peer Reviewed
• [Presentation] Distinct Characteristics of Indole-3-Acetic Acid and Phenylacetic Acid, Two Common Auxins in Plants. (2017)
  • Author(s): Satoko Sugawara, Kiyoshi Mashiguchi, Keita Tanaka, Shojiro Hishiyama, Tatsuya Sakai, Kousuke Hanada, Kaori Kinoshita-Tsujimura, Hong Yu, Xinhua Dai, Yumiko Takebayashi, Noriko Takeda-Kamiya, Tatsuo Kakimoto, Hiroshi Kawaide, Masahiro Natsume, Mark Estelle, Yunde Zhao, 他3名
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学
• [Presentation] 師部特異的な転写因子か維管束ハターンを制御する仕組み (2017)
  • Author(s): 財前美希,久米佐和,Ye Zhang,光田展隆,吉積毅,近藤陽一,高木優,松井南,柿本辰男
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学
• [Presentation] 師部形成に関与する転写因子の解析 (2016)
  • Author(s): Zaizen M, Kume S, Zhang Y, Mitsuda N, Yoshizumi T, Kondo Y, Takagi M, Matsui M, Kakimoto T.
  • Organizer: 第５７回植物生理学会年会
  • Place of Presentation: 岩手大学
  • Year and Date: 2016-03-18
• [Presentation] 初期器官発生における低分子量 G タンパク質の機能解析 (2016)
  • Author(s): 哈斯 其木格、柿本辰男
  • Organizer: 第５７回植物生理学会年会
  • Place of Presentation: 岩手大学
  • Year and Date: 2016-03-18
• [Presentation] CLE(CLAVATA3/ESR-LIKE)9 controls cell proliferation in stomatal lineage. (2016)
  • Author(s): Qian P, Minobe A, Ishida T, Sawa S, Kakimoto T.
  • Organizer: 第５７回植物生理学会年会
  • Place of Presentation: 岩手大学
  • Year and Date: 2016-03-18
• [Presentation] Transcription factors that may regulate phloem companion-cell development (2016)
  • Author(s): Zaizen Miki,Kume Sawa,Zhang Ye,Mitsuda Nobutaka,Yoshizumi Takeshi,Kondo Yoichi,Ohme-Takagi Masaru,Matsui Minami,Kakimoto Tatsuo
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学
  • Year and Date: 2016-03-16
• [Presentation] Molecular mechanisms that determine pericycle cell identity. (2016)
  • Author(s): Ye Zhang, Nobutaka Mitsuda, Chuan-Ming Yeh, Takeshi Yoshizumi, Yoichi Kondo, Masaru Takagi, Minami Matsui, Tatsuo Kakimoto
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学
• [Presentation] 初期器官発生における低分子量Gタンパク質の機能解析 (2016)
  • Author(s): ハスチムグ、柿本辰男
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学
• [Presentation] シロイヌナズナの根の光屈性におけるオーキシン輸送、生合成、シグナル伝達の機能解析 (2016)
  • Author(s): 木村太郎、芳賀健、志水三田尾悌、竹林裕美子、林謙一郎、Yunde Zhao, 柿本辰男、笠原博幸、酒井達也
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学
• [Presentation] CLE (CLAVATA3/ESR-like) 9 controls cell proliferation in stomatal cell lineage (2016)
  • Author(s): 銭平平、美濃部彩子、石田喬志、澤進一郎
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学
• [Presentation] Regulation of plant epidermal development in response to biotic and abiotic stresses. (2015)
  • Author(s): Qian P, Kumari A, Kakimoto T.
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 新潟
  • Year and Date: 2015-09-07
• [Presentation] Molecular mechanisms that determine pericycle cell identity. (2015)
  • Author(s): Zhang Y, Mitsuda N, Yeh C-M, Yoshizumi T, Kondo Y, Takagi M, Matsui M, Kakimoto T.
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 新潟
  • Year and Date: 2015-09-06
• [Presentation] Molecular mechanisms that determine pericycle cell identity (2015)
  • Author(s): Ye Zhang, Nobutaka Mitsuda, Chuan-Ming Yeh, Takeshi Yoshizumi, Yoichi Kondo, Masaru Takagi, Minami Matsui, Tatsuo Kakimoto
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 新潟市
• [Presentation] Regulation of plant epidermal development in response to biotic and abiotic stresses (2015)
  • Author(s): Pingping Qian, Archana Kumari, Tatsuo Kakimoto
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 新潟市
• [Presentation] 篩部形成に関与する転写因子の解析 (2015)
  • Author(s): Miki Zaizen, Sawa Kume, Ye Zhang, Nobutaka Mitsuda, Takeshi Yoshizumi, Yoichi Kondo, Masaru Takagi, Minami Matsui, Tatsuo Kakimoto
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 新潟市
• [Presentation] シロイヌナズナの根の光屈性におけるオーキシン輸送、生合成、シグナル伝達の機能解析 (2015)
  • Author(s): 木村太郎、芳賀健、志水三田尾悌、竹林裕美子、林謙一郎、Yunde Zhao, 柿本辰男、笠原博幸、酒井達也
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 新潟市
• [Presentation] Active growth reduction of leaves under osmotic stress is executed by decreasing the SPEECHLESS protein in Arabidopsis. (2014)
  • Author(s): Kumari A, Pawan J, Bergmann D, Kakimoto T.
  • Organizer: 25th International Conference on Arabidopsis Research
  • Place of Presentation: バンクーバー、カナダ
  • Year and Date: 2014-07-30
  • Int'l Joint Research
• [Presentation] Active growth reduction of leaves under osmotic stress is executed by decreasing SPEECHLRDD protein inArabidopsis (2014)
  • Author(s): Kumari A., Pawan J., Bergman D., Kakimoto, T.
  • Organizer: 25th International Conference on arabiodopsis Research
  • Place of Presentation: バンクーバー（カナダ）
  • Year and Date: 2014-07-30
• [Presentation] Molecular Mechanisms that determine pericycle cell identity. (2014)
  • Author(s): Zhang Y, Mitsuda N, Yoshizumi T, Kondo Y, Takagi M, Matsui M, Kakimoto T.
  • Organizer: International Symposium on Auxin and Cytokinin in Plant Development.
  • Place of Presentation: Prague, Czech Republic
  • Year and Date: 2014-07-01
  • Int'l Joint Research
• [Presentation] Auxin sensitivities and functions of Aux/IAAs. (2014)
  • Author(s): Shimizu Y and Kakimoto T.
  • Organizer: International Symposium on Auxin and Cytokinin in Plant Development.
  • Place of Presentation: Prague, Czech Republic
  • Year and Date: 2014-07-01
  • Int'l Joint Research
• [Presentation] Molecular Mechenisms Determine Pericycle Cell Idenntity (2014)
  • Author(s): Zhang Y., Kakimoto T. et al
  • Organizer: Auxins and Cytokinins in Plant Development 2014
  • Place of Presentation: プラハ（チェコ）
  • Year and Date: 2014-07-01
• [Presentation] Auxin sensitivities and functions of Aux/IAAs (2014)
  • Author(s): Shimizu Y., Kakimoto T.
  • Organizer: Auxins and Cytokinins in Plant Development 2014
  • Place of Presentation: プラハ（チェコ）
  • Year and Date: 2014-07-01
• [Presentation] 環境に応答した発生プログラム調節 (2014)
  • Author(s): 柿本 辰男，Kumari Archana，Bergmann Dominique，Jewaria Pawan
  • Organizer: 第55回 日本植物生理学会年会
  • Place of Presentation: 富山大学（富山）
  • Year and Date: 2014-03-20
  • Invited
• [Presentation] Molecular Mechanisms that Determine Pericycle Cell Identity (2014)
  • Author(s): Ye Zhang, Nobutaka Mitsuda, Takeshi Yoshizumi, Yoichi Kondo, Masaru Takagi, Minami Matsui, Tatsuo Kakimoto
  • Organizer: 第55回 日本植物生理学会年会
  • Place of Presentation: 富山大学（富山）
  • Year and Date: 2014-03-18
• [Book] 新しい植物ホルモンの科学 第3版 (2017)
  • Author(s): 柿本辰男、他22名
  • Total Pages: 181
  • Publisher: 講談社
• [Book] 新しい植物ホルモンの科学 第３版 (2016)
  • Author(s): 柿本辰男、他22名
  • Total Pages: 183
  • Publisher: 講談社