| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2013: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
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| Outline of Final Research Achievements |
At present, it is widely believed that phytochrome regulates the transcription of light-responsive genes by modulating the activity of several transcription factors. However, we performed mRNA-seq analysis and revealed that phytochrome controls not only transcription, but also alternative splicing, to approximately the same degree at the genomic level in Arabidopsis. Moreover, we experimentally showed that phytochrome-induced alternative splicing changes of SPA3, the negative regulator of light signaling, physiologically contributed to promoting light responses of plants. From these results, we demonstrated that phytochrome directly and simultaneously regulates two different aspects of gene expression, namely transcription and alternative splicing to mediate light responses in plants.
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