• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Elucidation of common regulatory mechanisms for nodule and meristem development

Research Project

Project/Area Number 25291066
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionNational Institute for Basic Biology

Principal Investigator

Kawaguchi Masayoshi  基礎生物学研究所, 共生システム研究部門, 教授 (30260508)

Co-Investigator(Renkei-kenkyūsha) Suzaki Takuya  基礎生物学研究所, 共生システム研究部門, 助教 (40575825)
Takeda Naoya  基礎生物学研究所, 共生システム研究部門, 助教 (60571081)
Research Collaborator Okamoto Satoru  基礎生物学研究所, 研究員
Soyano Takashi  基礎生物学研究所, 研究員
Fujita Hironori  基礎生物学研究所, 研究員
Sasaki Takema  総合研究大学院大学, 基礎生物学専攻, 大学院生
Yoro Emiko  総合研究大学院大学, 基礎生物学専攻, 大学院生
Takahara Masahiro  総合研究大学院大学, 基礎生物学専攻, 大学院生
Nishida Hanna  総合研究大学院大学, 基礎生物学専攻, 大学院生
Mori Tomoko  基礎生物学研究所, 技術職員
Tanaka Sachiko  基礎生物学研究所, 技術職員
Matsubayashi Yoshikatsu  名古屋大学, 生命理学研究科, 教授
Shinohara Hidefumi  名古屋大学, 生命理学研究科, 助教
Sakakibara Hitoshi  理化学研究所, 環境資源科学研究センター, グループリーダー
Hayashi Makoto  理化学研究所, 環境資源科学研究センター, グループリーダー
Kojima Mikiko  理化学研究所, 環境資源科学研究センター, 技師
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Keywords根粒形成 / 遠距離シグナリング / 糖修飾ペプチド / 根由来シグナル / シュート由来シグナル / 茎頂メリステム / サイトカイニン / ミヤコグサ / オートレギュレーション / CLEペプチド / 遠距離シグナル伝達 / 翻訳後修飾 / HAR1 / CLEペプチド
Outline of Final Research Achievements

Root nodules are controlled by the root-to-shoot-to-root negative feedback loop called autoregulation of nodulation (AON). In Lotus japonicus, AON is mediated by CLE-RS genes and the receptor kinase HAR1 that functions in the shoot. However, the mature structures of CLE-RS peptides and the molecular nature of CLE-RS/HAR1 signaling remain elusive. In this project we show that CLE-RS2 is a post-translationally arabinosylated glycopeptide derived from the CLE domain. Chemically synthesized CLE-RS glycopeptides cause significant suppression of nodulation and directly bind to HAR1 in an arabinose-chain-dependent manner. In addition, CLE-RS2 glycopeptide produced in the root is found in shoot xylem sap. We thus propose that CLE-RS glycopeptides are the long sought mobile signals responsible for the initial step of AON. In addition we show that, CLE-RS-HAR1 signaling activates the production of cytokinins in shoots, which have an SDI-like capacity to systemically suppress nodulation.

Report

(4 results)
  • 2016 Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (23 results)

All 2016 2015 2014 2013 Other

All Journal Article (15 results) (of which Peer Reviewed: 15 results,  Acknowledgement Compliant: 2 results,  Open Access: 2 results) Presentation (4 results) (of which Invited: 1 results) Book (4 results)

  • [Journal Article] Expression of the CLE-RS3 gene suppresses root nodulation in Lotus japonicus.2016

    • Author(s)
      Nishida H, Handa Y, Tanaka S, Suzaki T, Kawaguchi M.
    • Journal Title

      J Plant Res

      Volume: 129 Issue: 5 Pages: 909-19

    • DOI

      10.1007/s10265-016-0842-z

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Leguminous plants : inventors of root nodules to accommodate symbiotic bacteria (Review)2015

    • Author(s)
      *Takuya Suzaki, *Emiko Yoro, and Masayoshi Kawaguchi (*co-firstauthors)
    • Journal Title

      International Review of Cell and Molecular Biology

      Volume: 316 Pages: 111-158

    • DOI

      10.1016/bs.ircmb.2015.01.004

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RNA-seq Transcriptional Profiling of an Arbuscular Mycorrhiza Provides Insights into Regulated and Coordinated Gene Expression in Lotus japonicus and Rhizophagus irregularis2015

    • Author(s)
      Handa Y, Nishide H, Takeda N, Suzuki Y, Kawaguchi M, Saito K.
    • Journal Title

      Plant Cell Physiol

      Volume: 56 Issue: 8 Pages: 1490-511

    • DOI

      10.1093/pcp/pcv071

    • NAID

      120007101327

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Shoot HAR1 mediates nitrate inhibition of nodulation in Lotus japonicus2015

    • Author(s)
      Okamoto S, Kawaguchi M.
    • Journal Title

      Plant Signal Behav.

      Volume: 10 Issue: 5 Pages: e1000138-e1000138

    • DOI

      10.1080/15592324.2014.1000138

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Shoot-derived cytokinins systemically regulate root nodulation.2014

    • Author(s)
      Sasaki, T., Suzaki, T., Soyano, T., Kojima, M., Sakakibara, H. and Kawaguchi, M.
    • Journal Title

      Nat. Commun.

      Volume: 5 Issue: 1 Pages: 4983-4983

    • DOI

      10.1038/ncomms5983

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] NODULE INCEPTION creates a long-distance negative feedback loop involved in homeostatic regulation of nodule organ production.2014

    • Author(s)
      Soyano, T., Hirakawa, H., Sato, S., *Hayashi, M., and Kawaguchi, M.
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 111 Issue: 40 Pages: 14607-14612

    • DOI

      10.1073/pnas.1412716111

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Endoreduplication-mediated initiation of symbiotic organ development in Lotus japonicus2014

    • Author(s)
      Takuya Suzaki, Momoyo Ito, Emiko Yoro, Shusei Sato, Hideki Hirakawa, Naoya Takeda, and Masayoshi Kawaguchi
    • Journal Title

      Development

      Volume: 141 Issue: 12 Pages: 2441-2445

    • DOI

      10.1242/dev.107946

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A positive regulator of nodule organogenesis, NODULE INCEPTION, acts as a negative regulator of rhizobial infection in Lotus japonicus.2014

    • Author(s)
      Yoro, E., Suzaki, T., Toyokura, K., Miyazawa, H., Fukaki, H., and Kawaguchi, M.
    • Journal Title

      Plant Physiol.

      Volume: 165 Issue: 2 Pages: 747-758

    • DOI

      10.1104/pp.113.233379

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evolutionary dynamics of nitrogen fixation in the legume-rhizobia symbiosis.2014

    • Author(s)
      Fujita, H., Aoki, S., and Kawaguchi, M.
    • Journal Title

      PLoS One

      Volume: 9 Issue: 4 Pages: e93670-e93670

    • DOI

      10.1371/journal.pone.0093670

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Root nodulation: a developmental program involving cell fate conversion triggered by symbiotic bacterial infection.2014

    • Author(s)
      Suzaki, T., and Kawaguchi, M.
    • Journal Title

      Curr. Opin. Plant Biol.

      Volume: 21 Pages: 16-22

    • DOI

      10.1016/j.pbi.2014.06.002

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Root-derived CLE glycopeptides control nodulation by direct binding to HAR1 receptor kinase.2013

    • Author(s)
      Okamoto, S., Shinohara, H., Mori, T., Matsubayashi, Y., and Kawaguchi, M.
    • Journal Title

      Nat. Commun.

      Volume: 4 Issue: 1 Pages: 2191-2191

    • DOI

      10.1038/ncomms3191

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Leaf growth of Lotus japonicus hypernodulation mutant har1-4.2013

    • Author(s)
      Tanabata S, Ohtake N, Sueyoshi K, Kawaguchi M and Ohyama T.
    • Journal Title

      Journal of Theoretical Biology

      Volume: 66 Pages: 21-24

    • NAID

      120006745531

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Grafting analysis indicates that malfunction of TRICOT in the root causes a nodulation-deficient phenotype in Lotus japonicus.2013

    • Author(s)
      Suzaki, T. and Kawaguchi, M.
    • Journal Title

      Plant Signaling & Behavior

      Volume: 3 Issue: 4 Pages: 518-27

    • DOI

      10.1093/pcp/pct008

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Too much love, a novel Kelch repeat-containing F-box protein, functions in the long-distance regulation of the legume-Rhizobium symbiosis2013

    • Author(s)
      Takahara, M., Magori, S., Soyano, T., Okamoto, S., Yoshida, C., Yano, K., Sato, S., Tabata, S., Yamaguchi, K., Shigenobu, S., et al
    • Journal Title

      Plant Cell Physiol

      Volume: 54 Issue: 4 Pages: 433-447

    • DOI

      10.1093/pcp/pct022

    • NAID

      10031163838

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Two distinct EIN2 genes cooperatively regulate ethylene signaling in Lotus japonicus.2013

    • Author(s)
      Miyata K, Kawaguchi M and Nakagawa T.
    • Journal Title

      Planct Cell Physiol.

      Volume: 54 Pages: 1469-77

    • NAID

      10031202069

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] 根粒形成を負に制御する新たなCLEペプチドの同定2016

    • Author(s)
      西田帆那、半田佳宏、田中幸子、寿崎拓哉、川口正代司
    • Organizer
      第57回日本植物生理学会
    • Place of Presentation
      岩手大学(青森県盛岡市)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] Arabinosylated CLE peptides mediate global control of nodulation2014

    • Author(s)
      川口 正代司
    • Organizer
      COLD SPRING HARBOR ASIA Coference
    • Place of Presentation
      Cold Spring Habor Asia (China))
    • Year and Date
      2014-04-21 – 2014-04-25
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] CLEペプチドを介した根粒形成の遠距離制御機構

    • Author(s)
      川口正代司
    • Organizer
      平成25年度植物感染生理談話会
    • Place of Presentation
      北陸粟津温泉法師(石川県小松市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] CLE peptide signaling in long-distance control of nodulation

    • Author(s)
      Masayoshi Kawaguchi
    • Organizer
      18th International Congress on Nitrogen Fixation
    • Place of Presentation
      フェニックス シーガイア リゾート(宮崎県宮崎市)
    • Related Report
      2013 Annual Research Report
  • [Book] 葉と根のコミュニケーションによる根粒形成の遠距離制御2016

    • Author(s)
      川口正代司
    • Total Pages
      8
    • Publisher
      化学と生物(日本農芸化学会)
    • Related Report
      2015 Annual Research Report
  • [Book] Advances in Biology and Ecology of Nitrogen Fixation2014

    • Author(s)
      Soyano, T., and Kawaguchi, M.
    • Total Pages
      21
    • Publisher
      InTech
    • Related Report
      2014 Annual Research Report
  • [Book] The Lotus japonicus Genome2014

    • Author(s)
      Kawaguchi, M.
    • Total Pages
      6
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report
  • [Book] The Lotus japonicus Genome2014

    • Author(s)
      Kawaguchi, M., and Sandal, N.
    • Total Pages
      10
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report

URL: 

Published: 2013-05-21   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi