| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2015: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2013: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
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| Outline of Final Research Achievements |
A mechanism regulating the sperm swimming has not clearly been understood. In sea urchin sperm, calcium plays an important role for this regulation. At pH6-7, sperm does not show swimming and is immotile. Calcium dynamics of influx and efflux through flagellar membrane is known to be necessary for normal swimming and an inhibition of this dynamics brings about the inhibition of swimming movement.By using this characteristics of sperm at pH7 and 8 we compared the differences such as raft formation and behavior of membrane fluorescent probes. We found that the function of calcium efflux protein in the flagellar membrane, PMCA, requires formation of a so-called lipid raft with cooperative existence of flagellasialin. Actin was detected in the raft fraction of the sperm membrane obtained at pH8, but the role of actin has not been elucidated. Furthermore, we observed that a fluorescent probe detecting rafts moves along the flagella at pH8, suggesting a certain dynamic factor of the raft.
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