Mechanism regulating the swimming behavior of sperm by calcium- A role of actin

• Project/Area Number: 25291072
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Animal physiology/Animal behavior
• Research Institution: The University of Tokyo
• Principal Investigator: Shingyoji Chikako 東京大学, 理学(系)研究科(研究院), 准教授 (80125997)
• Co-Investigator(Kenkyū-buntansha): 北島 健 名古屋大学, 生物機能開発利用研究センター, 教授 (80192558)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2015: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2013: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
• Keywords: 生体膜 / ラフト / カルシウム / 精子 / ウニ / 生体分子 / 生理学 / 細胞 / アクチン / 膜ラフト

Outline of Final Research Achievements

A mechanism regulating the sperm swimming has not clearly been understood. In sea urchin sperm, calcium plays an important role for this regulation. At pH6-7, sperm does not show swimming and is immotile. Calcium dynamics of influx and efflux through flagellar membrane is known to be necessary for normal swimming and an inhibition of this dynamics brings about the inhibition of swimming movement.By using this characteristics of sperm at pH7 and 8 we compared the differences such as raft formation and behavior of membrane fluorescent probes. We found that the function of calcium efflux protein in the flagellar membrane, PMCA, requires formation of a so-called lipid raft with cooperative existence of flagellasialin. Actin was detected in the raft fraction of the sperm membrane obtained at pH8, but the role of actin has not been elucidated. Furthermore, we observed that a fluorescent probe detecting rafts moves along the flagella at pH8, suggesting a certain dynamic factor of the raft.

Research Products

• [Journal Article] Effects of external strain on the regulation of microtubule sliding induced by outer arm dynein of sea urchin sperm flagella (2017)
  • Author(s): Yoke, H. and Shingyoji, C.
  • Journal Title: J. Exp. Biol. Volume: 220 Pages: 1122-1134
  • DOI: 10.1242/jeb.147942
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Diverse subcellular localizations of the insect CMP-sialic acid synthetases. (2017)
  • Author(s): Wu D, Fujita A, Hamaguchi K, Delannoy P, Sato C, Kitajima K.
  • Journal Title: Glycobiology Volume: 27 Pages: 329-341
  • DOI: 10.1093/glycob/cww128
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effects of intronic single nucleotide polymorphisms (iSNPs) of a polysialyltransferase, ST8SIA2 gene found in psychiatric disorders on its gene products (2016)
  • Author(s): Hane M, Kitajima K, Sato C.
  • Journal Title: Biochem Biophys Res Commun. Volume: 478 Issue: 3 Pages: 1123-1129
  • DOI: 10.1016/j.bbrc.2016.08.079
  • Peer Reviewed / Open Access
• [Journal Article] The AMOR arabinogalactan sugar chain induces pollen tube competency to respond to ovular guidance (2016)
  • Author(s): Mizukami A., Inatsugi R., Jiao J., Kotake T., Kuwata K., Ootani K, Okuda S., Sankaranarayanan S., Sato Y., Maruyama D., Iwai H., Garenaux E., Sato C., Kitajima K., Tsumuraya Y., Mori H., Yamaguchi J., Itami K., Sasaki N. and Higashiyama T.
  • Journal Title: Curr. Biol. Volume: 26 Issue: 8 Pages: 1091-1097
  • DOI: 10.1016/j.cub.2016.02.040
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Relationship between ST8SIA2, polysialic acid and its binding molecules, and psychiatric disorders. (2016)
  • Author(s): Sato C, Hane M, Kitajima K.
  • Journal Title: Biochim Biophys Acta. Volume: 1860 Issue: 8 Pages: 1739-1752
  • DOI: 10.1016/j.bbagen.2016.04.015
  • Peer Reviewed / Open Access
• [Journal Article] Dynein arms are strain-dependent direction-switching force generators. (2015)
  • Author(s): Shingyoji, C., Nakano, I., Inoue, Y. and Higuchi, H.
  • Journal Title: Cytoskeleton Volume: 72 Issue: 8 Pages: 388-401
  • DOI: 10.1002/cm.21232
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Discovery, primary, and crystal structures and capacitation-related properties of a prostate-derived heparin-binding protein WGA16 from boar sperm (2015)
  • Author(s): Estelle Garenaux, Mayumi Kanagawa, Tomoyuki Tsuchiyama, Kazuki Hori, Takeru Kanazawa, Ami Goshima, Mitsuru Chiba, Hiroshi Yasue, Akemi Ikeda, Yoshiki Yamaguchi, Chihiro Sato, Ken Kitajima
  • Journal Title: Journal of Biological Chemistry Volume: 9 Issue: 9 Pages: 5484-5501
  • DOI: 10.1074/jbc.m114.635268
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Rapid trimming of cell surface polySia by exovesicular sialidase triggers release of preexisting surface neurotrophin. (2015)
  • Author(s): Sumida M, Hane M, Yabe U, Shimoda Y, Pearce OM, Kiso M, Miyagi T, Sawada M, Varki A, KITAJIMA K, Sato C.
  • Journal Title: J. Biol. Chem. Volume: 290 Issue: 21 Pages: 13202-13214
  • DOI: 10.1074/jbc.m115.638759
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Sulfatide-Hsp70 interaction promotes Hsp70 clustering and stabilizes binding to unfolded protein. (2015)
  • Author(s): Harada Y, Sato C, Kitajima K
  • Journal Title: Biomolecules Volume: 5 Issue: 2 Pages: 958-973
  • DOI: 10.3390/biom5020958
  • Peer Reviewed
• [Journal Article] Ganglioside contained in the neuronal tissue-enriched acidic protein of 22 kDa (NAP-22) fraction prepared from the detergent-resistant membrane microdomain of rat brain inhibits the phosphatase activity of calcineurin (2015)
  • Author(s): Kobayashi, Y. da Silva, R. Kumanogoh, H. Miyata, S. Sato, C. Kitajima, K. Nakamura, S. Morita, M. Hayashi, F. Maekawa, S.
  • Journal Title: J Neurosci Res Volume: 93 Issue: 9 Pages: 1462-1470
  • DOI: 10.1002/jnr.23599
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Protective effects of polysialic acid on proteolytic cleavage of FGF2 and proBDNF/BDNF. (2015)
  • Author(s): Hane M, Matsuoka S, Ono S, Miyata S, Kitajima K, and Sato C
  • Journal Title: Glycobiology Volume: Oct;25 Issue: 10 Pages: 1112-1124
  • DOI: 10.1093/glycob/cwv049
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Roles of plasma membrane modification in the regulation of intracellular calcium in sea urchin spermatozoa. (2017)
  • Author(s): Izumi Nakano, Yuichi Inoue, Takeru Kanazawa, Chihiro Sato, Ken Kitajima, Kenichi Suzuki, Akihiko Kusumi, Chikako Shingyoji
  • Organizer: The 5th International Symposium on Dynamical Ordering of Biomolecular Systems for Creation of Integrated Functions
  • Place of Presentation: The University of Tokyo Komaba Campus (Tokyo, Shibuya-ku)
  • Year and Date: 2017-01-21
  • Int'l Joint Research
• [Presentation] Effects of mechanical deformation on the sliding velocity of microtubules interacted with outer arm dyneins of sea urchin sperm flagella. (2017)
  • Author(s): Takashi Fujiwara and Chikako Shingyoji
  • Organizer: The 5th International Symposium on Dynamical Ordering of Biomolecular Systems for Creation of Integrated Functions,
  • Place of Presentation: The University of Tokyo Komaba Campus (Tokyo, Shibuya-ku)
  • Year and Date: 2017-01-21
  • Int'l Joint Research
• [Presentation] Light-induced Ca2+-dependent flagellar response of sperm in the presence of pyrenebutyrate. (2017)
  • Author(s): Jittrapon Jirakulsomchok, Izumi Nakano, Chikako Shingyoji
  • Organizer: The 5th International Symposium on Dynamical Ordering of Biomolecular Systems for Creation of Integrated Functions,
  • Place of Presentation: The University of Tokyo Komaba Campus (Tokyo, Shibuya-ku)
  • Year and Date: 2017-01-21
  • Int'l Joint Research
• [Presentation] Critical conditions in inducing flagellar responses by mechanical deformation in demembranated motionless sea urchin sperm at low ATP. (2017)
  • Author(s): Yasuhide Izawa and Chikako Shingyoji
  • Organizer: The 5th International Symposium on Dynamical Ordering of Biomolecular Systems for Creation of Integrated Functions,
  • Place of Presentation: The University of Tokyo Komaba Campus (Tokyo, Shibuya-ku)
  • Year and Date: 2017-01-21
  • Int'l Joint Research
• [Presentation] Mechanical activity of dynein and its dynamical regulation underlying oscillatory movement of sperm flagella. (2017)
  • Author(s): C. Shingyoji, H. Yoke and Y. Izawa
  • Organizer: The 5th International Symposium on Dynamical Ordering of Biomolecular Systems for Creation of Integrated Functions,
  • Place of Presentation: The University of Tokyo Komaba Campus (Tokyo, Shibuya-ku)
  • Year and Date: 2017-01-21
  • Int'l Joint Research
• [Presentation] Factors important for mechanical deformation induced cyclical bending in motionless sea urchin sperm flagella at low ATP concentrations. (2016)
  • Author(s): Y. Izawa and C. Shingyoji
  • Organizer: The 22nd International Congress of Zoology and The 87th Meeting of Zoological Society of Japan,
  • Place of Presentation: Okinawa Institute of Science and Technology Graduate University (Okinawa , Onna-son) and Okinawa Convention Center (Okinawa , Ginowan)
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] Effects of mechanical deformation on the velocity of microtubule gliding movement induced by isolated outer arm dynein of sea urchin sperm flagella. (2016)
  • Author(s): T. Fujiwara and C. Shingyoji
  • Organizer: The 22nd International Congress of Zoology and The 87th Meeting of Zoological Society of Japan,
  • Place of Presentation: Okinawa Institute of Science and Technology Graduate University (Okinawa , Onna-son) and Okinawa Convention Center (Okinawa , Ginowan)
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] Strain-sensitive regulation of mechanical activities of dyneins underlying oscillatory movement of sperm flagella. (2016)
  • Author(s): C. Shingyoji, H. Yoke, I. Nakano, Y. Inoue, H. Higuchi
  • Organizer: The 22nd International Congress of Zoology and The 87th Meeting of Zoological Society of Japan,
  • Place of Presentation: Okinawa Institute of Science and Technology Graduate University (Okinawa , Onna-son) and Okinawa Convention Center (Okinawa , Ginowan)
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] A Novel Method to Induce Light-controlled Calcium-dependent Flagellar Responses in the Presence of Pyrenebutyrate in Sea Urchin Sperm. (2016)
  • Author(s): J. Jirakulsomchok, I. Nakano, C. Shingyoji
  • Organizer: The 22nd International Congress of Zoology and The 87th Meeting of Zoological Society of Japan,
  • Place of Presentation: Okinawa Institute of Science and Technology Graduate University (Okinawa , Onna-son) and Okinawa Convention Center (Okinawa , Ginowan)
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] Dynamic roles of plasma membrane protein in the regulation of intracellular calcium in sea urchin spermatozoa. (2016)
  • Author(s): I. Nakano, Y. Inoue, T. Kanazawa, C. Sato, K. Kitajima, C. Shingyoji
  • Organizer: The 22nd International Congress of Zoology and The 87th Meeting of Zoological Society of Japan,
  • Place of Presentation: Okinawa Institute of Science and Technology Graduate University (Okinawa , Onna-son) and Okinawa Convention Center (Okinawa , Ginowan)
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] Elusive Localization of the Insect CMP-sialic Acid Synthetases. (2016)
  • Author(s): Ken Kitajima
  • Organizer: The Sialoglyco 2016 conference
  • Place of Presentation: Santa Barbara, USA
  • Year and Date: 2016-11-14
  • Int'l Joint Research / Invited
• [Presentation] Glycan-mediated Interactions on Pig Sperm Lipid Rafts during Fertilization (2016)
  • Author(s): Ken Kitajima
  • Organizer: APMC11 / MST33 / AAT39 Conference
  • Place of Presentation: Phuket, Thailand
  • Year and Date: 2016-05-23
  • Int'l Joint Research / Invited
• [Presentation] Present status and future prospects of deaminoneuraminic acid (KDN) biology at the 30th anniversary of discovery. (2016)
  • Author(s): Ken Kitajima
  • Organizer: Frontiers in Sialic Acid Research Conference: From Structural Diversity to Functional Glycobiology
  • Place of Presentation: Bad Lauterberg, Germany
  • Year and Date: 2016-04-23
  • Int'l Joint Research / Invited
• [Presentation] Why does sialic acid deficiency cause the embryonic lethality in medaka fish, Oryzias latipes? (2016)
  • Author(s): Ken Kitajima
  • Organizer: Glycoscience Japan- The Netherlands Joint Seminar 2016
  • Place of Presentation: Leiden, The Netherlands
  • Year and Date: 2016-04-19
  • Int'l Joint Research / Invited
• [Presentation] 低濃度ATP中で動いていないウニ精子鞭毛に機械的変形により誘導される鞭毛反応の解析 (2015)
  • Author(s): 伊澤寧秀，真行寺千佳子
  • Organizer: 日本動物学会第86回大会
  • Place of Presentation: 朱鷺メッセ（新潟県新潟市）
  • Year and Date: 2015-09-17
• [Presentation] ウニ精子鞭毛外腕ダイニンによる微小管滑り運動の制御に対する外力の効果 (2015)
  • Author(s): 餘家 博，真行寺千佳子
  • Organizer: 日本動物学会第86回大会
  • Place of Presentation: 朱鷺メッセ（新潟県新潟市）
  • Year and Date: 2015-09-17
• [Presentation] ウニ精子の運動制御における細胞膜脂質ラフトの役割 (2015)
  • Author(s): 中野 泉，井上裕一，鈴木健一，楠見明弘，真行寺千佳子
  • Organizer: 日本動物学会第86回大会
  • Place of Presentation: 朱鷺メッセ（新潟県新潟市）
  • Year and Date: 2015-09-17
• [Presentation] Mechanical activity of dynein and its dynamical ordering underlying oscillatory movement of sperm flagella (2015)
  • Author(s): Shingyoji, C.
  • Organizer: The 3rd International Symposium on Dynamical Ordering of Biomolecular Systems for Creation of Integrated Functions
  • Place of Presentation: 志摩市（合歓の郷）
  • Year and Date: 2015-01-10 – 2015-01-11
  • Invited
• [Presentation] ウニ精子鞭毛ダイニン分子の出す力の特性と外力の効果 (2014)
  • Author(s): 真行寺千佳子、中野 泉、井上裕一、樋口秀男
  • Organizer: 日本動物学会第85回大会
  • Place of Presentation: 仙台市（東北大学）
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] ウニ精子のカルシウム依存性鞭毛運動制御における脂質ラフト形成 (2014)
  • Author(s): 中野 泉、井上裕一、金澤 尊、北島 健、真行寺千佳子
  • Organizer: 日本動物学会第85回大会
  • Place of Presentation: 仙台市（東北大学）
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] ウニの体のミクロな世界 (2014)
  • Author(s): 渡辺友美、藤原貴史、真行寺千佳子
  • Organizer: 日本動物学会第85回大会
  • Place of Presentation: 仙台市（東北大学）
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] ウニ精子の機械刺激反応における細胞内カルシウムの役割 (2013)
  • Author(s): 中野泉、西出功、真行寺千佳子
  • Organizer: 日本動物学会第84回大会
  • Place of Presentation: 岡山市（岡山大学）
• [Presentation] Mechanical activity of dynein and its regulation underlying oscillatory movement of sperm flagella. (2013)
  • Author(s): Shingyoji, C.
  • Organizer: International Workshop Dynein 2013
  • Place of Presentation: Kobe (Orbis Hall)
  • Invited
• [Presentation] The role of intracellular calcium in the regulation of flagellar response to mechanical stimulation in sea urchin spermatozoa. (2013)
  • Author(s): Nakano, I., Nishide, I., Shingyoji, C.
  • Organizer: International Workshop Dynein 2013
  • Place of Presentation: Kobe (Orbis Hall)
• [Presentation] Effects of tail binding on the ATPase activity and motile activity in outer arm dyneins of sea urchin sperm flagella. (2013)
  • Author(s): Wada, M., Shingyoji, C.
  • Organizer: International Workshop Dynein 2013
  • Place of Presentation: Kobe (Orbis Hall)
• [Presentation] Effects of mechanical deformation on the initiation of beating in sea urchin sperm flagella. (2013)
  • Author(s): Izawa, Y., Shingyoji, C.
  • Organizer: International Workshop Dynein 2013
  • Place of Presentation: Kobe (Orbis Hall)
• [Book] Muscle Contraction and Cell Motility: Fundamentals and Developments (2016)
  • Author(s): C. Shingyoji
  • Total Pages: 447
  • Publisher: Pan Stanford Publishing
• [Book] Methods in Enzymology, vol. 524, Cilia Part A (edited by Wallace F. Marshall) (2013)
  • Author(s): Shingyoji, C.
  • Total Pages: 397
  • Publisher: Academic Press, UK
• [Remarks] 進化細胞生物学研究室 研究内容
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/hikaku/research/index.html